SNX27 Antibody - BSA Free

Western Blot: SNX27 Antibody [NBP1-45283] - Analysis of SNX27 on mouse brain extracts.

Immunocytochemistry/Immunofluorescence: SNX27 Antibody [NBP1-45283] - Detection of SNX27 (Green) in Hela cells using NBP1-45283. Nuclei (Blue) are counterstained using Hoechst 33258.

Immunohistochemistry: SNX27 Antibody [NBP1-45283] - IHC analysis of SNX27 in mouse bladder using DAB with hematoxylin counterstain.

Western Blot: SNX27 Antibody [NBP1-45283] - Analysis of SXN27 antibody in A. 293T + tagged SNX27 protein and B. 293T empty vector.

Simple Western: SNX27 Antibody [NBP1-45283] - Simple Western lane view shows a specific band for SNX27 in 0.5 mg/ml of Human Cerebellum (left) and Mouse Cerebellum (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Bv, Ze
• Applications: WB, Simple Western, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SNX27 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide made to an internal portion of human SNX27 (within residues 450-500). [Swiss-Prot# Q96L92]
• Localization: Cytoplasm cytosol. Early endosome. Note: In T lymphocytes, recruited from the cytosol to sorting endosomes by phosphoinositide-3-kinase products.
• Predicted Species: Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SNX27
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation reported in scientific literature (PMID 32413996)
  • Simple Western 10 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: In Western blot, a band is seen at approx. 65 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Human Cerebellum and Mouse Cerebellum lysate 0.5 mg/mL, separated by Size, antibody dilution of 10 ug/mL. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  SMARCD3 Antibody (1G6) - Azide and BSA Free

• Control Peptide:

  SNX27 Peptide (NBP1-45283PEP)

Reactivity Notes

Predicted to react with rat, bovine and Zebrafish based on 100% sequence homology. Use in Rat reported in scientific literature (PMID:32413996).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SNX27 Antibody - BSA Free

• KIAA0488MGC126873
• methamphetamine-responsive transcript 1
• MGC126871
• MGC20471
• MRT1
• MY014
• sorting nexin family member 27
• sorting nexin-27

Background

SNX27 belongs to SNX family of proteins which are unified by the presence of a phox domain, a phospholipid-binding motif, and are involved in intracellular sorting as well as trafficking. SNX27 was initially discovered in neocortex of rat as a developmentally regulated psychostimulant-inducible novel gene, which was named mrt1 (methamphetamine-responsive transcript 1), having two splice forms mrt1a (SNX27a) and mrt1b (SNX27b) that vary only in their C-terminal ends. In SNX family, SNX27 is the only SNX member containing a PDZ domain and it interact with the PDZ-bm of a few membrane proteins through its PDZ domain to regulate their intracellular sorting. SNX27 modulates the early endosomal trafficking of 5-HT4(a)R [5-hydroxytryptamine type-4(a) receptor], Kir3 (G protein-gated potassium) channels, NR2C (N-methyl-D-aspartate receptor 2C) etc., and has also been suggested to facilitate efficient recycling of beta2AR/beta2-adrenoreceptor to the plasma membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SNX27 Antibody (NBP1-45283)(2)

Publications using NBP1-45283

• Choi HJ, Jang HJ, Park E et al. Sorting Nexin 27 Regulates the Lysosomal Degradation of Aquaporin-2 Protein in the Kidney Collecting Duct Cells 2020-05-13 [PMID: 32413996] (IP, Rat)
• Ish-Shalom E, Meirow Y, Sade-Feldman M et al. Impaired SNX9 Expression in Immune Cells during Chronic Inflammation: Prognostic and Diagnostic Implications. J. Immunol. 2016-01-01 [PMID: 26608909] (WB, Mouse)

Bioinformatics

• Gene Symbol: SNX27
• Entrez:
  • 81609(Human)
  • 76742(Mouse)
• Uniprot:
  • Q96L92(Human)
  • Q3UHD6(Mouse)