SOCS-1 Antibody (5T6Z10)

Immunohistochemistry: SOCS-1 Antibody (5T6Z10) [NBP3-35060] - Immunohistochemistry analysis of paraffin-embedded Human colon using SOCS-1 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: SOCS-1 Antibody (5T6Z10) [NBP3-35060] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using SOCS-1 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: SOCS-1 Antibody (5T6Z10) [NBP3-35060] - Immunofluorescence analysis of MCF7 cells using SOCS-1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: ELISA, ICC/IF, IHC
• Clone: 5T6Z10
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SOCS-1 Antibody (5T6Z10) Summary

• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SOCS-1 (NP_003736.1).
  
  Sequence:
  MVAHNQVAADNAVSTAAEPRRRPEPSSSSSSSPAAPARPRPCPAVPAPAPGDTHFRTFRSHADYRRITRASALLDACGFYWGPLSVHGAHERLRAEPVGT
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SOCS1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for SOCS-1 Antibody (5T6Z10)

• CIS1
• Cish1
• JAB
• JABTec-interacting protein 3
• JAK binding protein
• JAK-binding protein
• SOCS1
• SOCS-1
• SOCS-1CIS1
• SSI-1
• SSI-1CISH1
• SSI1cytokine-inducible SH2 protein 1
• STAT induced SH3 protein 1
• STAT-induced STAT inhibitor 1
• suppressor of cytokine signaling 1
• TIP3
• TIP-3
• TIP3Cish1

Background

The SOCS (Suppressor of cytokine signaling) family of proteins has at least 8 members; SOCS1 to SOCS7 and CIS (cytokine inducible SH2 containing protein). SOCS1 has a role in the negative regulation of cytokines that signal through the JAK/STAT3 pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOCS1