Somatostatin R1/SSTR1 Antibody

Western Blot: Somatostatin Receptor 1 Antibody [NB300-120] - Detection of SSR1 from rat pancreas using NB300-120 (1:1000).

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Somatostatin R1/SSTR1 Antibody Summary

• Immunogen: A synthetic peptide from the C-terminus of rat Somatostatin Receptor 1, conjugated to cysteine. [UniProt# P28646]
• Specificity: Specific to Somatostatin Receptor I (SSR1) and does not react with Somatostatin Receptor II (SSR2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SSTR1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000-1:5000
• Application Notes: This Somatostatin Receptor 1 antibody is useful for Immunoprecipitation and Western blot, where a band is seen at 60 kDa representing Somatostatin Receptor 1. This antibody is not suitable for use in Immunocytochemistry/Immunofluorescence.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Notes

Licensed to Novus Biologicals LLC under U.S. Patent No 5,998,154.

Alternate Names for Somatostatin R1/SSTR1 Antibody

• G-protein coupled receptor
• Somatostatin R1
• somatostatin receptor 1
• somatostatin receptor type 1
• SomatostatinR1
• SRIF-2
• SS1R
• SS-1-R
• SS1-R
• SSR1
• SSTR1

Background

Somatostatin is a widely expressed circulating peptide hormone that has been involved in different biological processes including hormone secretion inhibition, neuronal transmission modulation, regulation of cell growth etc., and somatostatin's effects are mediated by a family of GPCRs "somatostatin Receptor-1 to -5" (SSTR1-5) that are differentially expressed in tumours and normal tissues including CNS/PNS, endocrine system and digestive canal. Once these receptors are activated, somatostatin exerts well-characterized direct/indirect antiproliferative effects in a variety of epithelial and endocrine cells. Somatostatin directly antagonizes the mitogenic action of EGF in pancreatic tumor cells and antagonizes additional tyrosine kinases in other cell types. SSTR1 belongs to GPCR1 family and it is a somatostatin receptor with higher affinity for somatostatin-14 than -28. SSTR1 is coupled to phosphotyrosine phosphatase and Na+/H+ exchanger via pertussis toxin insensitive G proteins, and is widely expressed in normal tissues as well as tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SSTR1
• Entrez:
  • 6751(Human)
  • 20605(Mouse)
  • 25033(Rat)
• Uniprot:
  • P28646(Rat)