Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974] - SPHK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- SPHK1 antibody NBP1-22974 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1 antibody NBP1-22973, which recognizes an upstream epitope

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Sphingosine Kinase 1/SPHK1 Antibody - BSA Free Summary

• Immunogen: sphingosine kinase 1
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPHK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 5 -15 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: This antibody is useful for western blot and immunoprecipitation. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:28175299).
• Theoretical MW: 42.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28175299).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

• EC 2.7.1.91
• SK1
• sphingosine kinase 1
• SPHK1
• SPHKSK 1
• SPK 1
• SPK
• SPK1

Background

Sphingosine kinase 1 (SPHK1) catalyzes the phosphorylation of sphingosine to yield sphingosine-1-phosphate (SPP). SPP functions as a lipid signaling molecule that mediates both intra- and extra-cellular responses. SPHK1 activity may play a role in several cellular processes such as apoptisis, proliferation, inflammation, and migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sphingosine Kinase 1/SPHK1 Antibody (NBP1-22974)(1)

Publication using NBP1-22974

• Moruno-Manchon JF, Uzor NE, Blasco-Conesa MP et al. Inhibiting sphingosine kinase 2 mitigates mutant Huntingtin-induced neurodegeneration in neuron models of Huntington disease Hum Mol Genet. 2017-03-31 [PMID: 28175299] (ICC/IF, WB, Rat)

Bioinformatics

• Gene Symbol: SPHK1
• Entrez:
  • 8877(Human)
• Uniprot:
  • Q2M3C7(Human)