SSEA-3 Antibody (MC-631) - BSA Free

Immunocytochemistry/Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832] - PSC characterisation. Human PSC-1 characterized for typical undifferentiated colony morphology in expression of pluripotency markers Nanog (Catalog # AF1997), OCT-3/4 (Catalog # AF1759), SSEA-3 (NB100-1832), SSEA-4 (Catalog # MAB1435) after immunofluorescence staining. Corresponding nuclei stains with DAPI shown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-33899-6), licensed under a CC-BY license.

Flow Cytometry: SSEA-3 Antibody (MC-631) [NB100-1832] - A surface stain was performed on Jurkat cells with SSEA-3 Antibody (MC-631) NB100-1832AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunocytochemistry/Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832] - Analysis of human embryonic stem cells stained with SSEA-3 antibody detected with Alexa Fluor 488 anti-rat IgM secondary antibody.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: MC-631
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SSEA-3 Antibody (MC-631) - BSA Free Summary

• Immunogen: 4-8 cell stage mouse embryos.
• Marker: Embryonic Stem Cell Marker
• Specificity: Recognizes a carbohydrate epitope of SSEA-3
• Isotype: IgM
• Clonality: Monoclonal
• Host: Rat
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 10-20 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: This SSEA3 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen and paraffin sections, Immunoprecipitation and Western Blot. This antibody is CyTOF ready.

Reactivity Notes

This antibody recognizes the SSEA-3 that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: IgM purified

Alternate Names for SSEA-3 Antibody (MC-631) - BSA Free

• SSEA3
• SSEA-3
• Stage specific embryonic antigen 3
• Stage-specific embryonic antigen 3

Background

SSEA-3 (stage-specific embryonic antigen-3) is a member of SSEAs which are present on eggs as well as preimplantation embryos at specific stages of early development. SSEAs are glycan epitopes which resides on glycoproteins/glycolipids of mammalian cells, and the epitope of several of these antigens consists solely of sugar residues. SSEA-3 antigen was identified following immunization of rats with 4-8 cell mouse embryos and was present on glycolipids of human teratocarcinoma cells. During protein glycosylation, beta-1,3-galactosyltransferase 5 catalyzes the transfer of Gal to the terminal GalNAc unit of the globoside GB4, thereby synthesizing glycolipid GB5 which is SSEA-3. In its functions. SSEA-3 has been proposed to involve in the process of cell differentiation. SSEA3 expression is mostly restricted to the cytoplasm or apical surface of epithelial cells and it has been reported to express in various normal tissues with high expression levels in kidney. SSEA3 has been reported to be expressed in the ascites fluids from patients of hepatoma or pancreatic cancer, breast cancer including breast cancer stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SSEA-3 Antibody (NB100-1832)(8)

Publications using NB100-1832

• Filidou E, Kandilogiannakis L, Tarapatzi G et al. A Simplified and Effective Approach for the Isolation of Small Pluripotent Stem Cells Derived from Human Peripheral Blood Biomedicines 2023-03-05 [PMID: 36979766] (Immunocytochemistry/ Immunofluorescence, Human)
• Kaur S, Abu-Shahba AG, Paananen RO et al. Small non-coding RNA landscape of extracellular vesicles from human stem cells Sci Rep 2018-10-21 [PMID: 30341351] (IF/IHC, Human)
• Skottman H. Derivation and characterization of three new human embryonic stem cell lines in Finland In Vitro Cell Dev Biol Anim 2010-02-24 [PMID: 20177999]
• Hakala H, Rajala K, Ojala M et al. Comparison of biomaterials and extracellular matrices as a culture platform for multiple, independently derived human embryonic stem cell lines Tissue Eng Part A 2009-01-10 [PMID: 19132919] (ICC/IF, Human)
• Matin, MM et al. Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells. Stem Cells;22(5):659-68. 2004-01-01 [PMID: 15342930]
• Henderson, JK et al. Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells;20(4):329-37. 2002-01-01 [PMID: 12110702] (FLOW, Human)
• Przyborski SA. Isolation of human embryonal carcinoma stem cells by immunomagnetic sorting. Stem Cells;19(6):500-4. 2001-01-01 [PMID: 11713341] (ICC/IF, Human)
• Kannagi R, Cochran NA, Ishigami F et al. Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J;2(12):2355-61. 1983-01-01 [PMID: 6141938] (WB, Human)

Review for SSEA-3 Antibody (NB100-1832) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Gail M. Seigel IHC-P Human 08/07/2010

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: HeLa cells
• Species: Human
• Lot: Lot A

FAQs for SSEA-3 Antibody (NB100-1832). (Showing 1 - 1 of 1 FAQs).

1. Could you please let us know the accession number of the antigen protein for SSEA3 Antibody (Cat. No. NB100-1832)?
   • SSEA3 is a globoseries carbohydrate antigen found on glycolipids as well as glycopeptides on cellular surface of embryonic stem cells. Because it is not a protein in reality, it is not supposed to have an accession number. NB100-1832 was generated in rat host using murine 4-8 cell stage embryos as immunogen and this antibody specifically detects the SSEA3 antigen in various immunoassays.