Human STAT1 Luciferase - (LUCPorter™) Stable Reporter Cell Line

Product Discontinued

Human STAT1 Luciferase - (LUCPorter™) Stable Reporter Cell Line Summary

• Description: The STAT1 / LUCPorter reporter cell line can be used for screening of agonists, antagonists or signaling inhibitors related with the STAT1-involved signaling pathways
  
  Contents : 3 ~ 4 x 10^6 cells
  Biosafety Level : 2
• Immunogen: The STAT1 / LUCPorterTM reporter cell line is a stably transfected HeLa cell line which expresses an optimized Renilla luciferase reporter gene (RenSP) under the transcriptional control of the interferon (IFN)-gamma activation sequence-based STAT1 response element, so that the cell line is designed to measure the transcriptional activity of STAT1.
• Target Species: Human
• Growth Properties: Adherent Morphology : Epithelial-like
• RCL Type: Luciferase - (LUCPorter™)
• Host: HeLa
• Gene: STAT1

Applications/Dilutions

• Dilutions:
  • Ligand Activation
• Readout System:
  • LightSwitch Luciferase Assay Reagent NBP2-32066

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 3 ug/ml puromycin.

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the TLR3 reporter cell line.
- Wash hands after handling the reporter cell line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Human STAT1 Luciferase - (LUCPorter™) Stable Reporter Cell Line

• CANDF7
• DKFZp686B04100
• ISGF-3
• ISGF-3,91kD
• signal transducer and activator of transcription 1, 91kDa
• signal transducer and activator of transcription-1
• STAT1
• STAT91

Background

Transcription factor, Signal Transducer and Activator of Transcription-1 is activated through phosphorylation at tyrosine 701 in response to various cytokines and growth factors such as IFN-alpha, IFN-gamma, IL-6, EGF and PDGF. The phosphorylated STAT1 forms homodimers or heterodimers with STAT3, and the dimers translocate to nucleus in which DNA binding/promoter induction occurs. Unlike STAT3, STAT1 has been known to function as a tumor suppressor, triggering anti-proliferative and pro-apoptotic responses while enhancing anti-tumor immunity1,2. On the other hand, a recent study has shown that STAT1 activity is elevated in human and mouse breast cancers, promoting breast cancer progression3. The STAT1 induction by IFN-gamma is shown in Figure. In the test, IFN-gamma induced the transcriptional activity of STAT1 in a dose response manner (Figure).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

FAQs for STAT1 Reporter Cell Line (NBP2-29634). (Showing 1 - 1 of 1 FAQ).

1. Could you please tell me if your Human STAT1 Luciferase - (LUCPorter™) Stable Reporter Cell Line also respond to IFN-beta?
   • About whether or not the interferon (IFN)-gamma activation sequence-based STAT1 response element (upstream of the luciferase gene) can respond to IFN-beta , I obtained two pieces of information. According to the datasheet, it says that: "Transcription factor, Signal Transducer and Activator of Transcription-1 is activated through phosphorylation at tyrosine 701 in response to various cytokines and growth factors such as IFN-alpha, IFN-gamma, IL-6, EGF and PDGF (Background".  And from Uniprot, it says that: "Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. ..." (Function). Therefore, if IFN-alpha, IFN-beta, and IFN-gamma all can activate the transcription factor ATAT1. However whether or not they are interacting with the same cis-acting elements in the STAT1 promoter and NBP2-29634 should respond to IFN-beta. Unfortunately, IFN-gamma is the only one that has been tested. I anticipate that there are citations on the interactions of the members of IFN with the STAT1, and the citation(s) can be for sure to know whether NBP2-29634 can respond to IFN-beta. FYI, due to the expiration of the contract, NBP2-29634 has been discontinue; apologize for any inconvenience. If having further questions/inquires, please feel free to contact us again.

Bioinformatics

• Gene Symbol: STAT1