SUG1 Antibody

Western Blot: SUG1 Antibody [NB100-2572] - Detection of Human and Mouse SUG1 on HeLa whole cell lysate (5, 15, and 50 microgram), 293T (T; 50 microgram), and NIH3T3 (M; 50 microgram) using NB100-2572. SUG1 was also immunoprecipitated by rabbit SUG1 antibodies NB100-2573 and another SUG1 Ab.

Immunohistochemistry-Paraffin: SUG1 Antibody [NB100-2572] - Sample: FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbit anti- TRIP1(SUG1) used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SUG1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Thyroid Receptor Interactor 1 using the numbering given in entry NP_002796.4 (GeneID 5705).
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMC5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  HeLa Whole Cell Lysate
  SUG1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SUG1 Antibody

• MSUG1 protein
• p45
• p45/SUG26S protease regulatory subunit 8
• proteasome (prosome, macropain) 26S subunit, ATPase, 5
• proteasome 26S ATPase subunit 5
• Proteasome 26S subunit ATPase 5
• Proteasome subunit p45
• S8
• SUG-1
• SUG126S proteasome AAA-ATPase subunit RPT6
• Tat-binding protein homolog 10
• TBP10
• thyroid receptor interactor 1
• TRIP1Thyroid hormone receptor-interacting protein 1

Background

TRIP15 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP. TRIP15 is also involved in early stage of neuronal differentiation via its interaction with NIF3L1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SUG1 Antibody (NB100-2572)(1)

Publication using NB100-2572

• Koues OI, Dudley RK, Truax AD et al. Regulation of Acetylation at the Major Histocompatibility Complex Class II Proximal Promoter by the 19S Proteasomal ATPase Sug1. Mol Cell Biol;28(19):5837-5850. 2008-01-01 [PMID: 18662994]

Bioinformatics

• Gene Symbol: PSMC5
• Entrez:
  • 5705(Human)
  • 19184(Mouse)
• Uniprot:
  • NP_002796.4(Human)