TDP-43/TARDBP Antibody (671834) [Unconjugated]

Western blot shows lysates of A431 human epithelial carcinoma cell line and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.4 µg/mL of Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TDP-43/TARDBP at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TDP-43/TARDBP at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

TDP-43/TARDBP was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TDP-43/TARDBP was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NR8383 rat alveolar macrophage cell line, loaded at 0.2 mg/mL. A specific band was detected for TDP-43/TARDBP at approximately 54 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) . This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Immunoprecipitation was performed on cell lysate of HAP1 human near-haploid cell line using 2.0 μg of Mouse Anti-Human TDP-43 Monoclonal Antibody (Catalog # MAB7778) pre-coupled to protein G or protein A beads. Immunoprecipitated TDP-43/TARDBP was detected with a Rabbit Anti-TDP-43 antibody. The Ponceau stained transfers of each blot are shown. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitated. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC, IP, ICC/IF
• Clone: 671834
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TDP-43/TARDBP Antibody (671834) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TDP-43/TARDBP
  Met1-Thr103
  Accession # Q13148
• Specificity: Detects human TDP-43/TARDBP in direct ELISAs. Detects human, mouse, and rat TDP-43/TARDBP in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TARDBP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-25 ug/mL
  • Immunohistochemistry 1-10 ug/mL
  • Immunoprecipitation 2 ug/1 mg cell lysate
  • Simple Western 5 ug/mL
  • Western Blot 0.4-1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TDP-43/TARDBP Antibody (671834) [Unconjugated]

• ALS10
• ALS10TAR DNA-binding protein-43
• TAR DNA binding protein
• TARDBP
• TDP43
• TDP-43
• TDP-43TAR DNA-binding protein 43

Background

TDP-43 (TAR DNA binding protein of 43 kDa; gene name TARDBP) is a 414 amino acid (aa) nuclear RNA/DNA binding phosphoprotein that regulates transcription and splicing. TDP-43 is aberrantly expressed in most forms of amylotrophic lateral sclerosis and frontotemporal lobar degeneration. In these cases it is absent from the nucleus and accumulates, along with a truncated 20-28 kDa form, in ubiquitin-containing inclusion bodies. TDP-43 can also influence CFTR exon 9 skipping in cystic fibrosis. Within aa 1-103, human and mouse TDP-43 share 100% aa sequence identity, and 99% aa identity with rat TDP-43.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TDP-43/TARDBP Antibody (MAB7778)(7)

Publications using MAB7778

• Worrall, D;Ayoubi, R;Fotouhi, M;Southern, K;McPherson, PS;Laflamme, C;NeuroSGC/YCharOS/EDDU collaborative group, ;ABIF Consortium, ; The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence F1000Research 2023-06-20 [PMID: 37359785] (Western Blot, Human)
• T Feng, L Luan, II Katz, M Ullah, VM Van Deerli, JQ Trojanowsk, EB Lee, F Hu TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss Acta neuropathologica communications, 2022-03-14;10(1):33. 3/14/2022 [PMID: 35287730]
• K Uehara, M Koyanagi-A, T Koide, T Itoh, T Aoi Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids Stem Cell Reports, 2022-03-03;0(0):. 2022-03-03 [PMID: 35245440] (Western Blot, Mouse)
• Devoy A, Price G, De Giorgio F Et al. Generation and analysis of innovative genomically humanized knockin SOD1, TARDBP (TDP-43), and FUS mouse models iScience 12/1/2021 [PMID: 34988393]
• Koji Matsukawa, Michail S. Kukharsky, Sei-Kyoung Park, Sangeun Park, Naruaki Watanabe, Takeshi Iwatsubo et al. Long non-coding RNA NEAT1_1 ameliorates TDP-43 toxicity in in vivo models of TDP-43 proteinopathy RNA Biology 2021-11-02 [PMID: 33427561]
• Feng T, Mai S, Roscoe JM et al. Loss of TMEM106B and PGRN leads to severe lysosomal abnormalities and neurodegeneration in mice EMBO Rep. 8/10/2020 [PMID: 32852886]
• Tatyana A. Shelkovnikova, Michail S. Kukharsky, Haiyan An, Pasquale Dimasi, Svetlana Alexeeva, Osman Shabir et al. Protective paraspeckle hyper-assembly downstream of TDP-43 loss of function in amyotrophic lateral sclerosis Molecular Neurodegeneration 2018-12-01 [PMID: 29859124] (Western Blot, Human)

Review for TDP-43/TARDBP Antibody (MAB7778) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human brain cortex-formalin fixed paraffin embedded.

reviewed by: Verified Customer IHC-P Human brain cortex-formalin fixed paraffin embedded 10/25/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Paraffin section of human brain
• Species: Human brain cortex-formalin fixed paraffin embedded
• Lot: CHGW011806A

Comments

• Comments: Antigen retrieval performed-Heat mediated at pH9
  Concentration: 1 in 100 overnight at 4C
  Secondary antibody: Alexafluor 594 anti-mouse antibody (Thermo) at 1 in 250
  Primary and secondary antibodies prepared in Antibody Diluent/ background reducing from Agilent.

Bioinformatics

• Gene Symbol: TARDBP
• Uniprot:
  • Q13148()