SAM68 Antibody

Western Blot: SAM68 Antibody [NBP1-19151] - Whole cell lysate from HeLa cells. SAM68 was also immunoprecipitated by rabbit anti-SAM68 antibodies NBP1-19152 and NBP1-19153.

Western Blot: SAM68 Antibody [NBP1-19151] - Whole cell lysate from HeLa cells. SAM68 was also immunoprecipitated by rabbit anti-SAM68 antibodies NBP1-19152 and NBP1-19153.

Immunohistochemistry-Paraffin: SAM68 Antibody [NBP1-19151] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SAM68 used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB

• Reactivity: Hu, Bv, Ma
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SAM68 Antibody Summary

• Immunogen: The epitope this antibody maps to a region between residue 1 and 50 of human SAM68 (KH domain containing, RNA binding, signal transduction associated 1) NP_006550.1 (GeneID 10657).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KHDRBS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID: 22280896)
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID: 27180817). Bovine reactivity reported in scientific literature (PMID: 22280896).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SAM68 Antibody

• FLJ34027
• GAP-associated tyrosine phosphoprotein p62
• KH domain containing, RNA binding, signal transduction associated 1
• KH domain-containing, RNA-binding, signal transduction-associated protein 1
• KHDRBS1
• p21 Ras GTPase-activating protein-associated p62
• p62
• p68
• SAM68
• Sam68GAP-associated tyrosine phosphoprotein p62 (Sam68)
• Src-associated in mitosis 68 kDa protein

Background

Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export Function: Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SAM68 Antibody (NBP1-19151)(2)

Publications using NBP1-19151

• Mandal A, Mandal S, Park MH. Global quantitative proteomics reveal up-regulation of endoplasmic reticulum stress response proteins upon depletion of eIF5A in HeLa cells. Nature Publishing Group. 2016-05-16 [PMID: 27180817] (WB, Mammal)
• Lawrence P, Schafer EA, Rieder E. The nuclear protein Sam68 is cleaved by the FMDV 3C protease redistributing Sam68 to the cytoplasm during FMDV infection of host cells. Virology. 2012-03-30 [PMID: 22280896] (ICC/IF, WB, Bovine, Human)

Review for SAM68 Antibody (NBP1-19151) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/16/2015

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: KHDRBS1
• Entrez:
  • 10657(Human)
• Uniprot:
  • Q07666()