TetR Antibody - BSA Free

Western Blot: TetR Antibody [NB600-234] - Analysis of TetR using 23 kDa recombinant protein. Note the presence of a dimer.

• Reactivity: All-NA
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TetR Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal portion of Escherichia coli TetR protein (between residues 25-75) [UniProt P04483]
• Localization: TetR peptide conserved in all strains of gram negative bacteria
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100
• Application Notes: This TetR antibody is useful for Western Blot and ELISA.
• Control Peptide:

  TetR Peptide (NB600-234PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TetR Antibody - BSA Free

• pSC101 tetR
• Tet Repressor Protein
• Tet Repressor
• TetR
• tetracycline repressor protein

Background

Tetracycline repressor TetR (tetracycline repressor protein class B from transposon Tn10) belongs to the Tet/Cam family of bacterial regulators and it controls the expression of Tn10 encoded tetracycline resistance. When tetracycline is not present, TetR recognises its operator sequence, tetO, for repressing tetA expression (resistance gene) and its own expression. Upon entry into the cell, tetracycline binds a pocket within the protein core of TetR and induces a conformational change leading to reorientation of DNA binding heads, which in turn abolishes operator binding for triggering resistance gene expression. TetR repressed tetracycline resistance element is responsible for the maintenance of antibiotic resistance traits in strains of Escherichia coli which confers an advantage in selection to E. coli expressing tetracycline resistance. Moreover, TetR has attracted widespread interest because regulators belonging to this family control the expression of genes which are involved in development, fatty acid metabolism, drug resistance and many other cellular processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TetR Antibody (NB600-234)(2)

Publications using NB600-234

• Kobylarz MJ, Goodwin JM, Kang ZB et al. An iron-dependent metabolic vulnerability underlies VPS34-dependence in RKO cancer cells PLoS ONE 2020-08-24 [PMID: 32833964]
• Vercauteren K, Pasko RA, Gleyzer N et al. PGC-1-related coactivator: immediate early expression and characterization of a CREB/NRF-1 binding domain associated with cytochrome c promoter occupancy and respiratory growth. Mol Cell Biol. 2006-10-01 [PMID: 16908542] (WB)
  
  Details:
  WB (TR-expressing and wild-type control BALB/3T3 cells): Fig 8. Disease-specific gene repositioning in breast cancer.FISH, Table 1 and various figures throughout the publication.

Reviews for TetR Antibody (NB600-234) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Bacteria.

reviewed by: Khuynh Nguyen WB Bacteria 10/06/2023

Summary

• Application: Western Blot
• Sample Tested: Pellet
• Species: Bacteria
• Lot: A-4

reviewed by: Ashley Barry WB Human 12/01/2016

Summary

• Application: Western Blot
• Sample Tested: combinant cell lines
• Species: Human
• Lot: A-1

Bioinformatics

• Entrez:
  • 4924774(E. coli)
• Uniprot:
  • P04483(E. coli)