TEV Protease Antibody - BSA Free

Western Blot: TEV Protease Antibody [NBP1-97669] - No translocation was observed for TEV protease cargo alone. Western blots show the presence of the constructs at lower retention volumes during size exclusion chromatography both at pH 8 (Left) and pH 11 (Right), meaning they co-localize with the rest of the holotoxin even after prepore-to-pore transition and are not translocated. Note: Lower retention volume towards left of blot and higher retention volume toward right. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31748551/) licensed under a CC-BY license.

Western Blot: TEV Protease Antibody [NBP1-97669] - E.coli lysate containing recombinant TEV protease, NBP1-97669 used at 1:1000 dilution.

• Reactivity: V-Vi
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TEV Protease Antibody - BSA Free Summary

• Description: This product was protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This protein-A purified TEV Protease Antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant MBP-and-poly-His-tagged auto inactivation-resistant mutant TEV Protease. (Uniprot: P04517)
• Specificity: Assay by western blot showed no reactivity to MBP.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TEVgp1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1500-1:27000
  • Western Blot 1:500
• Application Notes: This protein A purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kDa in size corresponding to TEV by western blotting in the appropriate cell lysate or extract. Anti-TEV protease may be useful to detect residual TEV protease in preparations of recombinant proteins in which that protease may interfere with downstream manipulations.

Reactivity Notes

Reactive with Tobacco Etch Virus

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for TEV Protease Antibody - BSA Free

• NIa
• P1 Protease
• TEV Protease
• Tobacco Etch Virus Protease

Background

TEV protease, encoded by the Tobacco Etch Virus (TEV), is a 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the virus (TEV). It is widely used for cleaving fusion proteins because of its sequence specificity. It recognizes a linear epitope of the general form E-Xaa-Xaa-Y-Xaa-Q-(G/S). Cleavage occurs between Q and G or Q and S. The structure of TEV protease is similar to that of of the serine protease family. Like serine proteases, TEV protease utilizes a catalytic triad of residues to hydrolyze peptide bonds. The distinguishing feature of TEV protease, however, is that instead of the serine nucleophile in the triad Ser-Asp-His, there is a cysteine, which may explain the resistance of TEV protease to protease inhibitors which are commonly used. The strain used is the autoinactivation-resistant mutant S219V. The catalytic activity of the S219V mutant is approximately 2 fold greater than that of the wild-type protease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TEV Protease Antibody (NBP1-97669)(2)

Publications using NBP1-97669

• Si L, Shen Q, Li J et al. Generation of a live attenuated influenza A vaccine by proteolysis targeting Nature biotechnology 2022-07-04 [PMID: 35788567] (WB, Virus - HPV)
  
  Details:
  1:1,000 dilution for western blotting, 1:200 dilution for immunofluorescence staining
• Roderer D, Schubert E, Sitsel O et al. Towards the application of Tc toxins as a universal protein translocation system Nat Commun 2019-11-22 [PMID: 31748551] (WB)

FAQs for TEV Protease Antibody (NBP1-97669). (Showing 1 - 1 of 1 FAQs).

1. Our customer who purchased NBP1-97669 would like to ask if the positive control for WB can be provided? In addition, there seems no suggested positive control listed. Would you please also help suggest?
   • Purified enhanced TEV should be fine commercially available from companies such as Sigma, Genway or Invitrogen. The end-user can also test against lysates transfected with any TEV designated autoinactivation-resistant mutant S219V

Bioinformatics

• Gene Symbol: TEVgp1
• Uniprot:
  • P04517(Virus)