TFEB Antibody (BLR070G)

Images

 
Western Blot: TFEB Antibody (BLR070G) [NBP2-77360] - Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1,and BW5147.3 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-TFEB recombinant ...read more
Western Blot: TFEB Antibody (BLR070G) [NBP2-77360] - Whole cell lysate (50 ug) from HeLa, Hep-G2, MCF-7, Jurkat, andK-562 cells prepared using NETN lysis buffer. Antibody:Rabbit anti-TFEB recombinant monoclonal ...read more
Independent Antibodies: TFEB Antibody (BLR070G) [NBP2-77360] - Detection of human TFEB by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-TFEB recombinant monoclonal [BLR070G] (NBP2-77360) and ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IP
Clone
BLR070G
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

TFEB Antibody (BLR070G) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Immunogen was a peptide representing a region between residue 426 and the C-terminus (residue 476) of human Transcription factor EB using the numbering given in entry NP_009093.1 (Gene ID 7942).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
TFEB
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunoprecipitation 20 ul/mg lysate
  • Western Blot 1:1000
Application Notes
Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.

Packaging, Storage & Formulations

Storage
Store at 2 - 8 C / 1 year from date of receipt
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for TFEB Antibody (BLR070G)

  • ALPHATFEB
  • BHLHE35
  • Class E basic helix-loop-helix protein 35
  • T-cell transcription factor EB
  • TCFEB
  • TCFEBbHLHe35
  • TFEB
  • transcription factor EB

Background

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Array NBP2-77360

Research Areas for TFEB Antibody (NBP2-77360)

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Blogs on TFEB.

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Bioinformatics

Gene Symbol TFEB