TGN38 Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Mouse embryonic fibroblasts, fixed with 4% PFA washed with PBS, permeabilized with 0.2% Triton X-100. Primary antibody at 1:100 in 3% BSA in PBS. Secondary antibodies incubation for 2 hours. Image from a verified customer review.

Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32.

Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32

Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to TGN diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.

Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to TGN diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TGN38 Antibody - Azide and BSA Free Summary

• Description: PLEASE NOTE: 0.1mg sample size is provided in reconstituted format and the 0.5mg size is provided lyophilized. (Please see reconstitution instuctions).
• Immunogen: A synthetic peptide from mouse TGN38 conjugated to blue carrier protein was used as the antigen.
• Marker: TGN Marker
• Specificity: TGN38 (or TGN46, TGN48, TGN51 in human).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGOLN2
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:250
  • Immunohistochemistry 10-50 ug/ml
  • Western Blot 10-50 ug/ml
• Application Notes: The 0.1 mg size ships reconstituted and the 0.5 mg size ships lyophilized. ICC/IF reactivity reported in (PMID: 27505890) and a verified customer review.

Reactivity Notes

Marmoset (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Contains 0.02% benzalkonium chloride.
• Preservative: No Preservative
• Purity: Ammonium sulfate precipitation
• Reconstitution Instructions: Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Alternate Names for TGN38 Antibody - Azide and BSA Free

• TGN38
• TGN46
• TGN46TGN38 homolog
• TGN48MGC14722
• TGN51
• TGN5148, 51kD isoforms)
• Tgoln1
• Tgoln2
• trans-golgi network protein 2
• Trans-Golgi network protein TGN51
• Ttgn1

Background

The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack. The TGN is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Some surface receptors recycle between the cell surface and the TGN, suggesting that the TGN is also important in endocytic pathways. TGN46 cycles between the trans-Golgi network and the cell surface returning via endosomes. It is thought to be involved in regulating membrane traffic to and from trans-Golgi network. Three splice isoforms are found: TGN46, TGN48 and TGN51. Isoform TGN46 is widely expressed, isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells whilst isoform TGN51 is more abundant in fetal lung and kidney. TGN46 is a human specific protein. The rodent homologue of TGN46 is known as TGN38.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGN38 Antibody (NBP1-03495)(15)

Publications using NBP1-03495

• Saller BS, Wöhrle S, Fischer L et Al. Acute suppression of mitochondrial ATP production prevents apoptosis and provides an essential signal for NLRP3 inflammasome activation Immunity 2024-11-15 [PMID: 39571574]
• Tao Yu, Dan Hou, Jiaqi Zhao, Xuan Lu, Wendy K. Greentree, Qian Zhao, Min Yang, Don-Gerard Conde, Maurine E. Linder, Hening Lin NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation Cell reports 2024-05-22 [PMID: 38583156]
• Liu Y, Zhai H, Alemayehu H et al. Cryo-electron tomography of NLRP3-activated ASC complexes reveals organelle co-localization Nature communications 2023-11-09 [PMID: 37945612] (ICC/IF, Mouse)
  
  Details:
  1:250 ICC/IF dilution
• Andreeva L, David L, Rawson S et al. NLRP3 cages revealed by full-length mouse NLRP3 structure control pathway activation Cell 2021-12-22 [PMID: 34861190] (ICC/IF, Mouse)
• Petkovic M, Oses-Prieto JA, Burlingame A, Jan L TMEM16K is an interorganelle regulator of endosomal sorting Nat Commun 2020-07-05 [PMID: 32620747]
• Watson AR, Dai H, Zheng Y et al. mTORC2 Deficiency Alters the Metabolic Profile of Conventional Dendritic Cells Front Immunol 2019-07-02 [PMID: 31338091] (ICC/IF, Mouse)
• Shi CS, Nabar NR, Huang NN, Kehrl JH SARS-Coronavirus Open Reading Frame-8b triggers intracellular stress pathways and activates NLRP3 inflammasomes Cell Death Discov 2019-06-05 [PMID: 31231549] (ICC/IF)
• Fink, A;Mikulicic, S;Blaum, F;Reddehase, MJ;Florin, L;Lemmermann, NAW; Function of the cargo sorting dileucine motif in a cytomegalovirus immune evasion protein Med. Microbiol. Immunol. 2019-04-19 [PMID: 31004199] (ICC/IF, Mouse)
• Ren C, Yuan Q, Braun M et al. Leukocyte Cytoskeleton Polarization Is Initiated by Plasma Membrane Curvature from Cell Attachment Dev. Cell 2019-03-23 [PMID: 30930167] (ICC/IF, Mouse)
• Ansari IH, Longacre MJ, Stoker SW et al. Characterization of Acyl-CoA synthetase isoforms in pancreatic beta cells: Gene silencing shows participation of ACSL3 and ACSL4 in insulin secretion. Arch. Biochem. Biophys. 2017-02-11 [PMID: 28193492] (WB, Human)
• Daisuke Sugahara, Yuka Kobayashi, Yoshihiro Akimoto, Hayato Kawakami Mouse intestinal niche cells express a distinct alpha 1,2-fucosylated glycan recognized by a lectin from Burkholderia cenocepacia. Glycobiology 2018-06-12 [PMID: 28177462]
• Sugahara D Kobayashi Y Akimoto Y, Kawakami H. Mouse intestinal niche cells express a distinct a1,2-fucosylated glycan recognized by a lectin from Burkholderia cenocepacia. Glycobiology. 2016-11-22 [PMID: 27979999] (Mouse)
• Fernandez-Fernandez MR, Ruiz-Garcia D, Martin-Solana E et al. 3D electron tomography of brain tissue unveils distinct Golgi structures that sequester cytoplasmic contents in neurons. J. Cell. Sci. 2016-08-05 [PMID: 27505890] (ICC/IF, Mouse)
• Ansari IH, Longacre MJ, Paulusma CC et al. Characterization of P4 ATPase Phospholipid Translocases (Flippases) in Human and Rat Pancreatic Beta Cells: Their Gene Silencing Inhibits Insulin Secretion. J. Biol. Chem. 2015-08-03 [PMID: 26240149] (WB, Human)
• Ting CH, Wen HL, Liu HC et al. The Spinal Muscular Atrophy Disease Protein SMN Is Linked to the Golgi Network PLoS One 2012-01-01 [PMID: 23284781] (WB, ICC/IF, Mouse)

Review for TGN38 Antibody (NBP1-03495) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer immune-fluorescence Mouse 08/05/2019

Summary

• Application: Other
• Sample Tested: MEF
• Species: Mouse
• Lot: Rb0705-060908-WS

Comments

• Comments: MEF cells were fixed by 4% PFA in PBS, for 15 min at room temperature. The cells were washed with PBS, and permeabilized with 0.2% Triton X100 in PBS, for 5 min at room temperature. The cells were washed with PBS, and then incubated with the indicated antibodies diluted as 1:100 in 3% BSA in PBS, at 4 degree overnight. On the following day, the cells were washed with PBS, and incubated with corresponding secondary antibodies, for 2 h at room temperature. Then the cells were washed with PBS, and mounted with moviol.

Bioinformatics

• Gene Symbol: TGOLN2
• Entrez:
  • 10618(Human)
  • 22135(Mouse)
  • 192152(Rat)
• Uniprot:
  • Q62314(Mouse)