TGR5/GPBAR1 Antibody - BSA Free

Western Blot: TGR5/GPBAR1 Antibody [NBP2-23669] - Analysis of spleen lysate. (1) Pre-immune sera. TGR5/GPBAR1 antibody tested on (2) Human 1:1000, (3) Mouse 1:5000, and (4) Rat 1:5000 spleen lysates.

Immunocytochemistry/Immunofluorescence: TGR5/GPBAR1 Antibody [NBP2-23669] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TGR5/GPBAR1 Antibody NBP2-23669 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: TGR5/GPBAR1 Antibody [NBP2-23669] - Tissue section of mouse colon using TGR5/GPBAR1 antibody at 1:200 dilution. The signal was detected using HRP-labelled secondary antibody and DAB staining which followed counterstaining with hematoxylin. This TGR5/GPBAR1 antibody generated a very specific a cytoplasmic staining primarily in the columner epithelial cells. A subset of cells from mucosa muscularis and the lamina propria also showed TGR5 positivity. Some cells in all histological layers depicted a nuclear staining also.

Immunocytochemistry/Immunofluorescence: TGR5/GPBAR1 Antibody [NBP2-23669] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TGR5/GPBAR1 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: TGR5/GPBAR1 Antibody [NBP2-23669] - LCA-induced oxidative stress responses were mediated by TGR5/GPBAR1 and by CAR receptors. TGR5/GPBAR1, CAR, VDR, and PXR bile acid receptors were silenced in MCF7 cells by transiently transfecting the cells with the corresponding siRNA or a negative control siRNA. After 48 h, protein expressions of NRF2 and iNOS were determined by western blotting (n = 3). Data are plotted as mean +/- SEM. * p < 0.05, control vs. LCA/siRNA treated. (CAR, constitutive androstane receptor; FXR, farnesoid X-activated receptor; LCA, lithocholic acid; LXR, liver X nuclear receptor; NRF2, nuclear factor, erythroid 2-like 2; TGR5/GPBAR1, G protein-coupled bile acid receptor 1/Takeda G-protein coupled receptor; VDR, vitamin D receptor). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/9/1255), licensed under a CC-BY license.

Western Blot: TGR5/GPBAR1 Antibody - BSA Free [NBP2-23669] - LCA-induced oxidative stress responses were mediated by TGR5 & by CAR receptors. (A) The 4T1 cells were treated with 0.3 μm LCA & NF449, CINPA1, DY268, or GSK2033 at a final concentration of 5 μm for 48 h, then NRF2 protein expression was detected using western blotting (representative figure, n = 2). (B,C) TGR5, CAR, VDR, & PXR bile acid receptors were silenced in MCF7 cells by transiently transfecting the cells with the corresponding siRNA or a negative control siRNA. After 48 h, protein expressions of (B,C) NRF2 & (C) iNOS were determined by western blotting (n = 3). Data are plotted as mean ± SEM. * p < 0.05, control vs. LCA/siRNA treated. (CAR, constitutive androstane receptor; FXR, farnesoid X-activated receptor; LCA, lithocholic acid; LXR, liver X nuclear receptor; NRF2, nuclear factor, erythroid 2-like 2; TGR5/GPBAR1, G protein-coupled bile acid receptor 1/Takeda G-protein coupled receptor; VDR, vitamin D receptor). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31461945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Pm, Rb
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TGR5/GPBAR1 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide from C-terminus of the human TGR5/GPBAR1 protein [UniProt Q8TDU6]
• Predicted Species: Primate (98%), Rabbit (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPBAR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Knockdown Validated
  • Western Blot 1 - 2 ug/ml

Reactivity Notes

Bovine (86%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TGR5/GPBAR1 Antibody - BSA Free

• BG37
• BG37hBG37
• G protein-coupled bile acid receptor 1
• GPBA
• GPBAR1
• GPCR19
• GPR131
• G-protein coupled bile acid receptor 1
• G-protein coupled bile acid receptor BG37
• G-protein coupled receptor GPCR19
• M-BAR
• M-BARhGPCR19
• membrane bile acid receptor
• Membrane-type receptor for bile acids
• MGC40597
• TGR5
• TGR5GPCR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGR5/GPBAR1 Antibody (NBP2-23669)(18)

Publications using NBP2-23669

• Zhong S, Liu F, Giniatullin R et al. Blockade of CCR5 suppresses paclitaxel-induced peripheral neuropathic pain caused by increased deoxycholic acid Cell reports 2023-11-28 [PMID: 37948181] (WB, IHC, Rat)
  
  Details:
  WB dilution 1:1000; IHC dilution 1:200
• Biagioli M, Marchianò S, Di Giorgio C et al. Activation of GPBAR1 attenuates vascular inflammation and atherosclerosis in a mouse model of NAFLD-related cardiovascular disease Biochemical Pharmacology 2023-11-01 [PMID: 37926268] (IHC-P, ICC/IF, Human, Mouse)
• Zou Y, Ghaderpour A, Munkhbileg B et al. Taurodeoxycholate ameliorates DSS-induced colitis in mice International immunopharmacology 2023-07-14 [PMID: 37454634] (Immunocytochemistry/ Immunofluorescence)
• Han B, Lv X, Liu G et al. Gut microbiota-related bile acid metabolism-FXR/TGR5 axis impacts the response to anti-?4?7-integrin therapy in humanized mice with colitis Gut microbes 2023-07-11 [PMID: 37431863] (WB)
• Di Giorgio C, Bellini R, Lupia A et al. Discovery of BAR502, as potent steroidal antagonist of leukemia inhibitory factor receptor for the treatment of pancreatic adenocarcinoma Frontiers in oncology 2023-03-14 [PMID: 36998446] (ICC/IF, Human)
• Tawulie D, Jin L, Shang X et al. Jiang-Tang-San-Huang pill alleviates type 2 diabetes mellitus through modulating the gut microbiota and bile acids metabolism Phytomedicine : international journal of phytotherapy and phytopharmacology 2023-02-26 [PMID: 36870307]
• Zhao W, Chen L, Tan W et al. Mannan Oligosaccharides Promoted Skeletal Muscle Hypertrophy through the Gut Microbiome and Microbial Metabolites in Mice Foods 2023-01-12 [PMID: 36673449] (Mouse, )
• Wang LJ, Zhao GP, Wang XF et al. Glycochenodeoxycholate Affects Iron Homeostasis via Up-Regulating Hepcidin Expression Nutrients 2022-08-02 [PMID: 35956351] (WB, Mouse, Human)
  
  Details:
  Dilution used 1:1000
• Xu J, Xie S, Chi S et al. Protective effects of taurocholic acid on excessive hepatic lipid accumulation via regulation of bile acid metabolism in grouper Food & function 2022-02-24 [PMID: 35199809]
• Xu J, Li X, Yao X et al. Protective Effects of Bile Acids Against Hepatic Lipid Accumulation in Hybrid Grouper Fed a High-Lipid Diet Frontiers in Nutrition 2022-01-25 [PMID: 35145986] (Mouse, )
• Sun S, Guo H, Liang N et al. GPBAR1 Promotes Proliferation and Is Related to Poor Prognosis of High-Grade Glioma via Inducing MAFB Expression Histol Histopathol 2021-12-14 [PMID: 34904217]
• Zhai Z, Niu KM, Liu H Et al. Policosanol alleviates hepatic lipid accumulation by regulating bile acids metabolism in C57BL6/mice through AMPK-FXR-TGR5 cross-talk Journal of food science 2021-11-03 [PMID: 34730235] (IHC-Fr, Mouse)
• Zhai Z, Niu KM, Liu Y et al. The Gut Microbiota-Bile Acids-TGR5 Axis Mediates Eucommia ulmoides Leaf Extract Alleviation of Injury to Colonic Epithelium Integrity Frontiers in Microbiology 2021-08-18 [PMID: 34489916] (Mouse, )
• Trah J, Arand J, Oh J et al. Lithocholic bile acid induces apoptosis in human nephroblastoma cells: a non-selective treatment option Sci Rep 2020-11-23 [PMID: 33230229] (WB)
• Beck L De invloed van zwangerschapscholestase en de behandeling met Ursochol op de verhouding van macrofagen subset M1 en M2 en de TGR5 receptor Thesis 2017-06-01 (IF/IHC, Human)
• Miko E, Vida A, KovAcs T et al. Lithocholic acid, a bacterial metabolite reduces breast cancer cell proliferation and aggressiveness. Biochim. Biophys. Acta 2018-04-12 [PMID: 29655782] (Mouse)
• Meng Y, Li W, Hu C et al. Ginsenoside F1 administration promotes UCP1-dependent fat browning and ameliorates obesity-associated insulin resistance Food Science and Human Wellness 2023-11-01
• Hashmi SFUH THE MECHANISTIC AND ETIOLOGICAL LINK BETWEEN BILE ACID DYSREGULATION AND PRETERM BIRTH Thesis 2022-01-01 (WB, Mouse)

Bioinformatics

• Gene Symbol: GPBAR1
• Uniprot:
  • Q8TDU6()