TLR8 Antibody (44C143) - BSA Free

Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Intracellular expression of TLR8 in monocyte-derived macrophages (MDMs). MDMs which were incubated with PE-conjugated anti-TLR8 or isotype antibody control. Intracellular expression was determined by flow cytometry. Representative profiles were similar in four independent experiments (n=4 subjects). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0104039) licensed under a CC-BY license.

Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Expression of TLR8 in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry. HCMLs were stained using an intracellular staining protocol with TLR8 antibodies followed by relevant secondary fluorescent-conjugated antibodies. Filled histograms (purple) represent the isotype controls and the open histograms (red) indicate TLR8. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.

Western Blot: TLR8 Antibody (44C143) [NBP2-24917] - All the cell lines expressed mRNA for TLR8 (Figure 1) and displayed a strong expression of TLR8 protein (Figure 2). Lysates of human intestinal tissue and mouse splenocytes were used as positive controls. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: TLR8 Antibody (44C143) [NBP2-24917] - Formalin-fixed, paraffin-embedded human spleen stained with TLR8 antibody at 2 ug/ml.

Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - HEK cells expressing TLR8 were treated with ssRNA40-Alexa 546 followed by fixation, permeablization and staining for TLR8 using anti-TLR8 Alexa 488 conjugate or isotype control IgG1/Kappa Alexa 488. TLR8 detection is shown along the horizontal axis, and ssRNA40 Alexa 546 detection is shown along the vertical axis. Direct excitation (488 and 561 nm lasers) of both fluorophores (Alexa 488 and Alexa 546) allowed quantitation of cells staining positive for TLR8 (anti-TLR8-Alexa488) and cells that had taken up ssRNA40-Alexa 546. Single-stranded RNA40 binds TLR8 (flow cytometry FRET assay). A representative experiment is shown from three independent experiments with similar results. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0104039) licensed under a CC-BY license.

Western Blot: TLR8 Antibody (44C143) [NBP2-24917] - Analysis of TLR8 in human Ramos cell lysate. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Simple Western: TLR8 Antibody (44C143) [NBP2-24917] - Simple Western lane view shows a specific band for TLR8 in 0.5 mg/ml of Ramos (left) and Human Spleen (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody Image from the standard format of this antibody.

• Reactivity: Hu, Mu, Rb
• Applications: WB, Simple Western, DB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 44C143
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TLR8 Antibody (44C143) - BSA Free Summary

• Additional Information: Clone 44C143 was used by HLDA to establish CD designation.
• Immunogen: This antibody was developed against a KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLR8
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Dot Blot reported in scientific literature (PMID 27248820)
  • Flow (Cell Surface) reported in multiple pieces of scientific literature
  • Flow (Intracellular) reported in reported in multiple pieces of scientific literature (PMID 24836676)
  • Flow Cytometry 0.5-1 ug/10^6 cells
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Simple Western 20 ug/ml
  • Western Blot 1-3 ug/ml
• Application Notes: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. This antibody is CyTOF ready.
• Agonist:
  • Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand NBP2-26231

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS pH 7.4
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TLR8 Antibody (44C143) - BSA Free

• CD288 antigen
• CD288
• MGC119599
• MGC119600
• TLR8
• toll-like receptor 8

Background

Toll-like receptor 8 (TLR8) is a member of the TLR family of receptors that play a role in innate immune system activation and the recognition of pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs) (1,2). TLRs are type I membrane receptors that can be expressed on either the cell surface or internally, on endosomes (1,2). TLR8 is an endosomal receptor and is activated by pathogenic single stranded (ss) RNA (1-3). TLR8 is located on the X chromosome and is expressed mostly in monocytes/macrophages, neutrophils, and myeloid dendritic cells (1-3). Structurally, TLR8 consists of an extracellular domain, a cysteine-rich region, and transmembrane domain, and a Toll/Interleukin-1 receptor homology (TIR) domain (3,4). The extracellular domain contains a N-terminal leucine rich repeat (LRRNT) and a C-terminal LRR (LRRCT) which have 26 LRRs between them, each approximately 20-30 amino acids (aa), and a Z-loop between LRR14 and LRR15 (3). The primary isoform of the human TLR8 is synthesized as a protein 1041 aa in length with a theoretical molecular weight of ~120 kDa (4).

TLR8 is highly similar to TLR7 and both pathways are mediated by the adapter protein MyD88 to signal through IFN regulatory factor 7 (IRF7) and nuclear factor (NF)-kappaB (1-3,5). However, TLR7 recognizes guanosine and GU-rich ssRNA, while TLR8 recognizes uridine and AU-rich sequences (2,5). TLR7/TLR8 agonists, including derivatives of the immunostimulatory imiquimod, have been shown to be a promising cancer therapy capable of providing anticancer signals to antigen presenting cells (APCs), with many agonists being tested in both pre-clinical and clinical trials (6). Similarly, studies suggest that agonists for TLR8, in combination with other individual TLR agonists and antagonists, may also be useful for treating inflammatory allergic diseases, such as allergic rhinitis (7).

References

1. Sakaniwa, K., & Shimizu, T. (2020). Targeting the innate immune receptor TLR8 using small-molecule agents. Acta crystallographica. Section D, Structural biology, 76(Pt 7). https://doi.org/10.1107/S2059798320006518

2. Cervantes, J. L., Weinerman, B., Basole, C., & Salazar, J. C. (2012). TLR8: the forgotten relative revindicated. Cellular & molecular immunology. https://doi.org/10.1038/cmi.2012.38

3. Ohto, U., Tanji, H., & Shimizu, T. (2014). Structure and function of toll-like receptor 8. Microbes and infection. https://doi.org/10.1016/j.micinf.2014.01.007

4. Uniprot (Q9NR97)

5. Jannuzzi, G. P., de Almeida, J., Paulo, L., de Almeida, S. R., & Ferreira, K. S. (2020). Intracellular PRRs Activation in Targeting the Immune Response Against Fungal Infections. Frontiers in cellular and infection microbiology. https://doi.org/10.3389/fcimb.2020.591970

6. Frega, G., Wu, Q., Le Naour, J., Vacchelli, E., Galluzzi, L., Kroemer, G., & Kepp, O. (2020). Trial Watch: experimental TLR7/TLR8 agonists for oncological indications. Oncoimmunology. https://doi.org/10.1080/2162402X.2020.1796002

7. Golshiri-Isfahani, A., Amizadeh, M., & Arababadi, M. K. (2018). The roles of toll like receptor 3, 7 and 8 in allergic rhinitis pathogenesis. Allergologia et immunopathologia. https://doi.org/10.1016/j.aller.2017.09.026

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR8 Antibody (NBP2-24917)(50)

Publications using NBP2-24917

• Hiltunen N, Kemi N, Väyrynen JP et Al. Toll-like receptors 1-9 in small bowel neuroendocrine tumors-Clinical significance and prognosis PLoS One 2024-05-06 [PMID: 38709790]
• Campbell GR, Rawat P, Teodorof-Diedrich C, Spector SA IRAK1 inhibition blocks the HIV-1 RNA mediated pro-inflammatory cytokine response from microglia The Journal of general virology 2023-05-01 [PMID: 37256770]
• Grant R. Campbell, Pratima Rawat, Stephen A. Spector Pacritinib Inhibition of IRAK1 Blocks Aberrant TLR8 Signalling by SARS-CoV-2 and HIV-1-Derived RNA Journal of Innate Immunity 2023-01-01 [PMID: 35785771]
• de Barros De Luccia T, Ono E, Menon R et al. The effect of Gestational Diabetes Mellitus on the fetal compartment Journal of Reproductive Immunology 2021-03-01 [PMID: 33836321] (IHC-P, Human)
• Li Y, Chen X, Jin R et al. Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs Science advances 2021-02-05 [PMID: 33627421] (WB, Mouse)
• Menendez D, Snipe J, Marzec J et al. p53-responsive TLR8 SNP enhances human innate immune response to respiratory syncytial virus J. Clin. Invest. 2019-08-20 [PMID: 31430261] (FLOW)
• Evankovich J, Lear T, Baldwin C et al. Toll-Like Receptor 8 Stability is Regulated by Ring Finger 216 in response to circulating MicroRNAs Am. J. Respir. Cell Mol. Biol. 2019-08-06 [PMID: 31385713]
• Deng G, Ge J, Liu C et al. Impaired expression and function of TLR8 in chronic HBV infection and its association with treatment responses during peg-IFN-a-2a antiviral therapy. Clin Res Hepatol Gastroenterol. [PMID: 28236535] (IF/IHC)
  
  Details:
  Citation using the PE form of this antibody.
• Helminen O, Huhta H, Kauppila JH et al. Localization of nucleic acid-sensing toll-like receptors in human and mouse pancreas. APMIS. 2016-12-28 [PMID: 28028829] (IF/IHC, Human)
• Helminen O, Huhta H, Lehenkari Petri P. Nucleic acid-sensing Toll-like receptors 3, 7 and 8 in esophageal epithelium, Barrett's esophagus, dysplasia and adenocarcinoma Oncoimmunology 2016-07-29 [PMID: 27467941]
• Huhta H, Helminen O, Kauppila JH et al. The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer J. Histochem. Cytochem. 2016-08-01 [PMID: 27370795] (IHC-P, Human)
• Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination. Oncotarget. 2016-07-05 [PMID: 27248820] (Cytometric Bead Assay Standard, Human)
• Jamin A, Dehoux L, Dossier C et al. Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome Clin Exp Immunol. [PMID: 26123900] (FLOW, Human)
• Chatillon JF Direct Toll-Like Receptor 8 signaling increases the functional avidity of human CD8+ T lymphocytes generated for adoptive T cell therapy strategies Immun Inflamm Dis. 2015-03-01 [PMID: 25866635] (FLOW, Human)
  
  Details:
  Citation using the PE format of this antibody.
• Zimmermann M, Aguilera FB, Castellucci M et al. Chromatin remodelling and autocrine TNFalpha are required for optimal interleukin-6 expression in activated human neutrophils. Nat Commun. [PMID: 25616107] (FLOW, Human)
  
  Details:
  Citation using the PE form of this antibody.
• Tengroth L, Arebro J, Kumlien Georen S et al. Deprived TLR9 Expression in Apparently Healthy Nasal Mucosa Might Trigger Polyp-Growth in Chronic Rhinosinusitis Patients. PLoS ONE. 2014-08-19 [PMID: 25133733] (FLOW, Human)
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
• Camacho-Sandoval R, Del Rio Estrada PM, Rivero-Arrieta A et al. Differential partial activation phenotype and production of TNF-a by conventional dendritic cells in response to LPS in HIV+ viremic subjects and HIV+ controllers Clin Exp Immunol [PMID: 25130456] (FLOW, Human)
  
  Details:
  Used the PE form of this antibody.
• Bernard MA, Han X, Inderbitzin S et al. HIV-Derived ssRNA Binds to TLR8 to Induce Inflammation-Driven Macrophage Foam Cell Formation. PLoS ONE 2014-08-05 [PMID: 25090652] (FLOW, Human)
  
  Details:
  Using the Alexa Fluor (R) 488 conjugated version of NBP2-24917, catalog number NBP2-24917AF488.
• Green TL, Santos MF, Ejaeidi AA et al. Toll-like receptor (TLR) expression of immune system cells from metastatic breast cancer patients with circulating tumor cells. Exp Mol Pathol. 2014-08-01 [PMID: 24836676] (Flow Cytometry Control, Human)
  
  Details:
  Citation using the Azide Free format of this antibody.
• Youn JC, Shim HS, Lee JS et al. Detailed pathologic evaluation on endomyocardial biopsy provides long-term prognostic information in patients with acute myocarditis. Cardiovasc. Pathol. 2014-01-17 [PMID: 24529879] (IHC-P, Human)
  
  Details:
  Endomyocardial biopies from patients with acute myocarditis, Tables 2,3.
• Wang CH, Eng HL, Lin KH et al. Functional Polymorphisms of TLR8 Are Associated With Hepatitis C Virus Infection Immunology 2013-11-08 [PMID: 24205871] (FLOW, Human)
  
  Details:
  This citation used the PE version of this antibody.
• Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013-04-08 [PMID: 23593278] (WB, Flow Cytometry Control)
  
  Details:
  Antibodies cited [multiple myeloma (MM) cell lines, human intestine tissue lysate, PBMC, mouse splenocytes] for WB (Figs 2, 3, 4A, 4B, 5, Table 1) and Flow (Intracellular): Figs 4C, 6-8, Table 1: 1. TLR1 (IMG-5012) 2. TLR2 (IMG-416A)3. TLR3 (IMG-315A)4 TL
• Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013-01-01 [PMID: 23183897]
  
  Details:
  Antibodies cited in Fig 4B for flow cytometric analysis of TLR expression in human monocyte-derived dendritic cells (MDDC), CD14+ monocytes, myeloid DC, and plasmacytoid DC:1. TLR2-FITC, clone T2.1 (IMG-416C): Flow (cell surface)2. TLR4-PE, clone HTA124 (
• Berger M, Hsieh CY, Bakele M et al. Neutrophils express distinct RNA receptors in a non-canonical way. J Biol Chem. 2012-06-01 [PMID: 22532562] (Flow Cytometry Control, Flow-CS)
  
  Details:
  TLR8-PE (IMG-321D): Flow (intracellular) + Flow (cell surface), Fig 1A (human neutrophils) & Fig 1B (HL60).2. TLR8 (IMG-321A): IF confocal microscopy, Fig 1C (human neutrophils).3. FITC-conjugated goat anti-rabbit secondary Ab (Cat no 20302), Flow (intracellular) + Flow (cell surface), various Figs.4. FITC-conjugated donkey anti-rat secondary Ab (Cat no 20204), Flow (intracellular) + Flow (cell surface), various Figs.
• Pang Maoyin, Bala Shashi, Kodys Karen et al. Inhibition of TLR8- and TLR4-induced Type I IFN induction by alcohol is different from its effects on inflammatory cytokine production in monocytes BMC Immunol 2011-01-01 [PMID: 21962237] (FLOW, Human)
  
  Details:
  This citation used the FITC version of this antibody.
• Dasgupta G, Chentoufi AA, You S et al. Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses. Invest Ophthalmol Vis Sci. 2011-05-17 [PMID: 21273544]
  
  Details:
  Products cited: Flow (intracellular), Fig 1A ( CD4+CD25+ regulatory T cells purified from rabbit conjunctiva) & Fig 2A (CD11b+ cells isolated from rabbit conjunctiva): TLR2/CD282 PE (IMG-416D), TLR3/CD283 (IMG-315D), TLR4/CD284 (IMG-417D), TLR8/CD288 (IMG
• Schwab N, Zozulya AL, Kieseier BC et al. An imbalance of two functionally and phenotypically different subsets of plasmacytoid dendritic cells characterizes the dysfunctional immune regulation in multiple sclerosis. J Immunol. 2010-05-01 [PMID: 20357264] (Flow - IC, Human)
  
  Details:
  TLR8 (IMG-321A): Flow (intracellular), human pDC's, Fig 1.
• Camacho-Sandoval R, Del Rio Estrada PM, Rivero-Arrieta A et al. Differential partial activation phenotype and production of TNF-a by conventional dendritic cells in response to LPS in HIV+ viremic subjects and HIV+ controllers Clin Exp Immunol (FLOW, Human)
  
  Details:
  This publication used the PE conjugated form of this antibody (Cat# NBP2-24817).
• Chamberlain ND, Kim SJ, Vila OM et al. Ligation of TLR7 by rheumatoid arthritis synovial fluid single strand RNA induces transcription of TNF-alpha in monocytes. Ann Rheum Dis. 2013-03-01 [PMID: 22730373]
• Dasari P, Zola H, Nicholson IC. Expression of Toll-like receptors by neonatal leukocytes. Pediatr Allergy Immunol. 2011-03-01 [PMID: 21054549]
  
  Details:
  IMGENEX Products Cited: CD3 (IMG-6240A), TLR8-PE (IMG-321D). 1. Flow (intracellular and extracellular): Human monocytes and T lymphocytes derived from adult blood and cord blood, Fig 1A. 2. IF/ICC: Human Monocytes and lymphocytes from adult blood and cord
• van den Berk LC, Jansen BJ, Siebers-Vermeulen KG et al. Toll-like receptor triggering in cord blood mesenchymal stem cells. J Cell Mol Med. 2009-09-01 [PMID: 20196781] (Human)
  
  Details:
  flow (cell surface) cytometry: TLR5 (IMG-663A), TLR6 (IMG-304), TLR8 (IMG-321). Human mesenchymal stem cells, Fig 1C.
• Biasin M, Piacentini L, Lo Caputo S et al. TLR activation pathways in HIV-1-exposed seronegative individuals. J Immunol. 2010-03-01 [PMID: 20124101] (Flow-CS)
  
  Details:
  TLR8 FITC (IMG-321C): Flow cell surface (CD4+ and CD14+ isolated from PBMC); Fig 3, Fig 4, Table 1.
• Panda A, Qian F, Mohanty S et al. Age-associated decrease in TLR function in primary human dendritic cells predicts influenza vaccine response. J Immunol. 2010-03-01 [PMID: 20100933]
• Clancy RM, Alvarez D, Komissarova E et al. Ro60-associated single-stranded RNA links inflammation with fetal cardiac fibrosis via ligation of TLRs: a novel pathway to autoimmune-associated heart block. J Immunol. 2010-02-15 [PMID: 20089705]
  
  Details:
  Antibodies cited: 1. TLR-7 (IMG-665A): Primary human macrophages derived from PBMCs, Flow (Intracellular): Fig 1A 2. TLR-8 FITC (IMG-321C): Primary human macrophages derived from PBMCs Flow (Intracellular): Fig 1A 3. TLR-7 (IMG-581A): Fetal cardiac fibrob
• Wu J, Meng Z, Jiang M et al. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology. [PMID: 19922426]
  
  Details:
  Citation using the PE/Cy5 form of this antibody.
• Pietschmann K, Beetz S, Welte S et al. Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes. Scand J Immunol. 2009-09-01 [PMID: 19703014]
• Vatakis Dimitrios N, Nixon Christopher C, Bristol Gregory, Zack Jerome A. Differentially stimulated CD4+ T cells display altered human immunodeficiency virus infection kinetics: implications for the efficacy of antiviral agents J Virol [PMID: 19129455] (FLOW)
  
  Details:
  Used the FITC form of this antibody.
• Sacre SM, Lo A, Gregory B et al. Inhibitors of TLR8 reduce TNF production from human rheumatoid synovial membrane cultures. J Immunol. 2008-12-01 [PMID: 19017992]
• Clark RA, Huang SJ, Murphy GF et al. Human squamous cell carcinomas evade the immune response by down-regulation of vascular E-selectin and recruitment of regulatory T cells. J Exp Med. 2008-09-29 [PMID: 18794336] (ICC/IF, Human)
  
  Details:
  Antibodies cited: 1. TLR8 (IMG-321A): IF/ICC (human squamous cell carcinoma tumor cells), Fig. 3A. 2. TLR7 (IMG-581A): IF/ICC (human squamous cell carcimona tumor cells), Fig. 3A.
• Cohen PA, Koski GK, Czerniecki BJ et al. STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cells. Blood. 2008-09-01 [PMID: 18577706]
  
  Details:
  Flow (intracellular), mouse bone marrow cells, Fig. 1E: 1. TLR3 FITC (IMG-315C) 2. TLR4 FITC (IMG-417C) 3. TLR7 (IMG-665A) 4. TLR8 FITC (IMG-321C) 5. TLR9 FITC (IMG-305C).
• Zuiderwijk-Sick EA, van der Putten C, Bsibsi M et al. Differentiation of primary adult microglia alters their response to TLR8-mediated activation but not their capacity as APC. Glia. 2007-11-15 [PMID: 17823968] (Human)
  
  Details:
  TLR8/CD288 FITC (IMG-321C). Flow (cell surface): Human macrophage colony stimulation factor (M-CSF) and granulocyte macrophage colony stimulating factor (GM-CSF) differentiated microglia, Fig 4C.
• Kaji M, Tanaka J, Sugita J et al. Ciprofloxacin inhibits lipopolysaccharide-induced toll-like receptor-4 and 8 expression on human monocytes derived from adult and cord blood. Ann Hematol. 2008-03-01 [PMID: 17724596] (Human)
  
  Details:
  TLR8/CD288 (IMG-321A). Human monocytes derived from adult and cord blood, data not shown see paper for more details.
• Murciano C, Villamon E, Yanez A et al. In vitro response to Candida albicans in cultures of whole human blood from young and aged donors. FEMS Immunol Med Microbiol. 2007-11-01 [PMID: 17714490]
  
  Details:
  The following anitbodies were used in flow (cell surface) cytometry: TLR2/CD282 FITC (IMG-416C), TLR4 FITC (IMG-417C), TLR6/CD286 FITC (IMG-304C). Human whole blood from both young and elderly volunteers, Fig 1. Note: The fluorescence mean intensity was m
• Wong CK, Cheung PF, Ip WK et al. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007-07-01 [PMID: 17332440] (WB, Human)
  
  Details:
  Human blood eosinophils and neutrophils from buffy coat: For WB, Fig. 1A: TLR1 (IMG-5012), TLR5 (IMG-664), TLR6 (IMG-304A), TLR7 (IMG-540), TLR8 (IMG-321A), TLR9 (IMG-305A). For Flow (Intracellular) and Flow (Surface), Fig. 1B: TLR1 (IMG-5021), TLR2 (IMG-416C), TLR3 (IMG-315C), TLR4 (IMG-417C), TLR5 (IMG-663C), TLR6 (IMG-304C), TLR7 (IMG-665A), TLR8 (IMG-321C), TLR9 (IMG-305C).
• Zola H, Swart B, Banham A et al. CD molecules 2006--human cell differentiation molecules J Immunol Methods 2007-01-30 [PMID: 17174972]
  
  Details:
  This citation used the PerCP version of this antibody.
• Xu S, Koldovsky U, Xu M et al High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production. Surgery. 2006-08-01 [PMID: 16904966]
  
  Details:
  Citation using the Azide Free version of this antibody.
• Levy O, Suter EE, Miller RL, Wessels MR. Unique efficacy of Toll-like receptor 8 agonists in activating human neonatal antigen-presenting cells. Blood. 2006-08-15 [PMID: 16638933] (FLOW)
  
  Details:
  TLR8-PE, (IMG-321D): Flow cytometry, Figure 5
• Lee J, Wu CC, Lee KJ et al. Activation of anti-hepatitis C virus responses via Toll-like receptor 7. Proc Natl Acad Sci U S A. 2006-02-07 [PMID: 16446426]
• Hart OM, Athie-Morales V, O'Connor GM, Gardiner CM. TLR7/8-mediated activation of human NK cells results in accessory cell-dependent IFN-gamma production. J Immunol. 2005-08-01 [PMID: 16034103] (WB, Human)
  
  Details:
  Antibodies cited: 1. TLR7 [(IMG-581A) WB, Fig. 1: TLR7 transfected cells and NKL human leukemia cell line. The specificity of the TLR7 antibody has been validated by WB using overexpressed TLR7 in 293T cells in Fig. 1.]. 2. TLR8 [(IMG-321A) WB, Fig. 1: TL
• Cognasse F, Hamzeh H, Chavarin P et al. Evidence of Toll-like receptor molecules on human platelets. Immunol Cell Biol. 2005-04-01 [PMID: 15748217]
  
  Details:
  TLR2-PE (IMG-416D), TLR4-PE (IMG-417D), TLR6 (IMG-304A), TLR8-PE (IMG-321D), TLR9-PE (IMG-305D). Applications: Intracellular Flow Cytomety and Cell Surface Flow Cytometry: Figs 1 and 2. A comparison of staining results, intracellular versus cell surface flow cytometry is shown. Cell type: Human platelets.

FAQs for TLR8 Antibody (NBP2-24917). (Showing 1 - 2 of 2 FAQs).

1. Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712
   • These antibodies are all purified from tissue culture supernatant.
2. Do you have any reference paperd to show this antibody (clone 44C143) reacts with mouse tissues?
   • Yes we do indeed have several papers cited with use in Mouse. I am not certain about your application so I am going to note the links to all of the papers that used the antibody in mouse.http://www.ncbi.nlm.nih.gov/pubmed/16446426https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3365979/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518890/I also have performed a blast comparison between the human immunogen sequence and the mouse and found it has 70 % homology. we would recommend the homology to be higher than 85% for predicting whether an antibody will work however there must be enough homology in the actual epitope.

Bioinformatics

• Gene Symbol: TLR8