TMEM8C Antibody - BSA Free

Immunohistochemistry-Paraffin: TMEM8C Antibody [NBP2-34175] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: TMEM8C Antibody [NBP2-34175] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.

Immunohistochemistry-Paraffin: TMEM8C Antibody [NBP2-34175] - Staining of human placenta shows moderate positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: TMEM8C Antibody [NBP2-34175] - Staining of human testis shows moderate positivity in spermatogonia.

• Reactivity: Hu, Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TMEM8C Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: KWLQKMKEKKGLYPDKSVYTQQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYMK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  TMEM8C Recombinant Protein Antigen (NBP2-34175PEP)

Reactivity Notes

Use in Mouse reported in scientific publication (PMID: 32664530).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TMEM8C Antibody - BSA Free

• MYOMAKER
• TMEM226
• Transmembrane Protein 226
• Transmembrane Protein 8C

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TMEM8C Antibody (NBP2-34175)(9)

Publications using NBP2-34175

• Tanimoto A, Yamaguchi Y, Kadowaki T et al. Rab44 negatively regulates myoblast differentiation by controlling fusogenic protein transport and mTORC1 signaling Journal of cellular biochemistry 2023-08-11 [PMID: 37566644]
• Oyakawa S, Yamaguchi Y, Kadowaki T et al. Rab44 deficiency accelerates recovery from muscle damage by regulating mTORC1 signaling and transport of fusogenic regulators Journal of cellular physiology 2023-08-10 [PMID: 37565627]
• Maeno T, Arimatsu R, Ojima K et al. Netrin-4 synthesized in satellite cell-derived myoblasts stimulates autonomous fusion Experimental cell research 2023-09-01 [PMID: 37437770] (WB, Mouse)
• Chen X, Guo J, Zhou Y et al. Modified contralateral C7 transfer to restore ulnar nerve function without sacrificing median nerve recovery: an experimental study The Journal of hand surgery, European volume 2023-05-19 [PMID: 37203387]
• Chen J, She Y, Chen R et al. Nec-1 alleviated the deleterious effect of CoCl2 on C2C12 myoblast differentiation and fusion via the mTOR pathway Tissue and Cell 2022-08-01 [PMID: 36081319] (ICC/IF, Mouse)
  
  Details:
  Dilution used for ICC 1:20
• Lilya Lehka, Małgorzata Topolewska, Dominika Wojton, Olena Karatsai, Paloma Alvarez-Suarez, Paweł Pomorski et al. Formation of Aberrant Myotubes by Myoblasts Lacking Myosin VI Is Associated with Alterations in the Cytoskeleton Organization, Myoblast Adhesion and Fusion Cells 2020-07-11 [PMID: 32664530] (Western Blot, Human)
• Lehka L, Topolewska M, Wojton D et al. Formation of Aberrant Myotubes by Myoblasts Lacking Myosin VI Is Associated with Alterations in the Cytoskeleton Organization, Myoblast Adhesion and Fusion Cells Jul 11 2020 [PMID: 32664530] (WB, ICC/IF, Mouse)
• Kneppers A, Verdijk L, de Theije C, Corten M, Gielen E, van Loon L, Schols A, Langen R. A novel in vitro model for the assessment of postnatal myonuclear accretion Skelet Muscle 2018-02-14 [PMID: 29444710] (Western Blot, Human)
• Kneppers A, Verdijk L, de Theije C, Corten M. A novel in vitro model for the assessment of postnatal myonuclear accretion. Skelet Muscle. 2018 Feb 14 [PMID: 29444710] (WB, Human)

Bioinformatics

• Gene Symbol: MYMK
• Uniprot:
  • A6NI61(Human)