TMIGD2 Antibody (953743) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with human TMIGD2/CD28H and eGFP was stained with and either (A) Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB83162) or (B) Mouse IgG2AIsotype Control (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

In a functional flow cytometry test,Recombinant Human B7-H7 Fc Chimera (Catalog #156-B7,500 ng/mL) binds to HEK293 human embryonic kidney cell linetransfected with recombinant human TMIGD2/CD28H (black dotted line). Binding iscompletely blocked (orange histogram) by 50 μg/mL of Mouse Anti-Human TMIGD2/CD28HMonoclonal Antibody (Catalog # MAB83162). Mouse IgG2B Isotype Control(Catalog #MAB003)at 50 μg/mL was used as a control (blue line). Cells were stained with MouseAnti-Human IgG1 Fc APC‑conjugated Monoclonal Antibody (Catalog #FAB110A).

Human PBMC were stained with either (A) Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB83162) or (B) Mouse IgG2A Isotype Control (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and Mouse Anti-Human CD3 PE-conjugated Monoclonal Antibody (FAB100P). View our protocol for Staining Membrane-associated Proteins.

Human PBMC were stained with either (A) Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB83162) or (B) Mouse IgG2A Isotype Control (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and Mouse Anti-Human CD16 PE-conjugated Monoclonal Antibody (FAB2546P). View our protocol for Staining Membrane-associated Proteins.

Human PBMC were stained with either (A) Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB83162) or (B) Mouse IgG2A Isotype Control (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and Mouse Anti-Human CD56 PE-conjugated Monoclonal Antibody (FAB2408P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 953743
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TMIGD2 Antibody (953743) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK-293-derived human TMIGD2/CD28H
  Met1-Gly150
  Accession # Q96BF3
• Specificity: Detects human TMIGD2/CD28H in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction 50 ug/mL
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TMIGD2 Antibody (953743) [Unconjugated]

• CD28H
• IGPR1
• IGPR-1
• MGC23244
• TMIGD2
• transmembrane and immunoglobulin domain containing 2
• transmembrane and immunoglobulin domain-containing protein 2

Background

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).

1. Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
2. Parry, R.V. et al. (2003) J.Immunol. 171:166.
3. Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
4. Huang, C. et al. (2003) Nature 424:219.
5. Kaneko, T. et al. (2008) Front. Biosci. 13:4938.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q96BF3()