TNF-alpha Antibody (52B83)

Immunohistochemistry-Paraffin: TNF-alpha Antibody (52B83) [NB600-1422] - TNF alpha in sheep tonsil FFPE. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: TNF-alpha Antibody (52B83) [NB600-1422] - TNF alpha in sheep tonsil FFPE. IHC-P image submitted by a verified customer review.

• Reactivity: Hu, Mu, Gp, Pm, Rb, Sh
• Applications: WB, ELISA, Flow, IHC
• Clone: 52B83
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

TNF-alpha Antibody (52B83) Summary

• Immunogen: The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha).
• Localization: Type 2 membrane protein or soluble form derived from the membrane by proteolytic cleavage.
• Specificity: This detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. Non-specific background staining is obesrved in connective tissues.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNF
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:10-1:100
• Application Notes: TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha (17.4 kDa) is not biologically active.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27390101). Sheep reactivity reported from a verified customer review. Rabbit reactivity reported in scientific literature (PMID: 28114570). Expected cross reactivity based on sequence identity: Guinea pig, Rhesus Monkey.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for TNF-alpha Antibody (52B83)

• APC1 protein
• Cachectin
• Cachetin
• DIF
• TNF
• TNF, monocyte-derived
• TNFA
• TNF-A
• TNFalpha
• TNF-alpha
• TNF-alphacachectin
• TNFATNF, macrophage-derived
• TNFG1F
• TNFSF1A
• TNFSF2
• TNFSF2TNF superfamily, member 2
• tumor necrosis factor (TNF superfamily, member 2)
• tumor necrosis factor alpha
• Tumor necrosis factor ligand superfamily member 2
• tumor necrosis factor
• tumor necrosis factor-alpha

Background

Tumor necrosis factor (TNF)-alpha is a pro-inflammatory cytokine belonging to the TNF superfamily that is secreted by monocytes/macrophages, T cell, and natural killer (NK), among others (1). TNF-alpha is synthesized as a 233 amino acid (aa) transmembrane protein (mTNF-alpha) with a theoretical molecular weight (MW) of 26 kDa (1,2) that forms a homotrimer. mTNF-alpha is cleaved by TNF-alpha converting enzyme (TACE) and released in its 157 aa, 17 kDa soluble form (sTNF-alpha) (1-5). Both mTNF-alpha and sTNF-alpha are capable of binding type 1 TNF receptors (TNFR1), whereas mTNF-alpha predominately binds to TNFR2 (1,2). TNF-alpha binding to its receptors causes receptor recruitment of adaptor proteins, formation of signaling complexes, and downstream signaling cascades (e.g. MAPK, NF-kappaB, and Caspase-8), leading to distinct cellular responses such as survival, proliferation, inflammation, necroptosis, and apoptosis (1-5).

TNF-alpha is critical for normal immune response; however, dysregulation of TNF-alpha production can result in various pathologies (2,4,5). Excessive production of pro-inflammatory cytokines including interleukin 1 (IL-1), IL-6, and TNF-alpha has been implicated in an array of autoimmune diseases like rheumatoid arthritis (RA), inflammatory bowel disease (IBD), and psoriasis (2,4,5). Anti-TNF monoclonal antibodies, including Infliximab, and soluble TNFR have been approved for the treatment of autoimmune and TNF-mediated diseases (5). Additionally, data suggests that TNF inhibitors can be beneficial for treating patients experiencing immune-related adverse events associated with immune checkpoint inhibitor cancer treatment (6).

References

1. Holbrook J, Lara-Reyna S, Jarosz-Griffiths H, McDermott M. Tumour necrosis factor signalling in health and disease. F1000Res. 2019;8:F1000 Faculty Rev-111. https://doi.org/10.12688/f1000research.17023.1

2. Jang DI, Lee AH, Shin HY, et al. The Role of Tumor Necrosis Factor Alpha (TNF-alpha) in Autoimmune Disease and Current TNF-alpha Inhibitors in Therapeutics. Int J Mol Sci. 2021;22(5):2719. https://doi.org/10.3390/ijms22052719

3. Horiuchi T, Mitoma H, Harashima S, Tsukamoto H, Shimoda T. Transmembrane TNF-alpha: structure, function and interaction with anti-TNF agents. Rheumatology (Oxford). 2010;49(7):1215-1228. https://doi.org/10.1093/rheumatology/keq031

4. Webster JD, Vucic D. The Balance of TNF Mediated Pathways Regulates Inflammatory Cell Death Signaling in Healthy and Diseased Tissues. Front Cell Dev Biol. 2020;8:365. https://doi.org/10.3389/fcell.2020.00365

5. Kalliolias GD, Ivashkiv LB. TNF biology, pathogenic mechanisms and emerging therapeutic strategies. Nat Rev Rheumatol. 2016; 12(1):49-62. https://doi.org/10.1038/nrrheum.2015.169

6. Chen AY, Wolchok JD, Bass AR. TNF in the era of immune checkpoint inhibitors: friend or foe?. Nat Rev Rheumatol. 2021;17(4):213-223. doi:10.1038/s41584-021-00584-4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TNF-alpha Antibody (NB600-1422)(12)

Publications using NB600-1422

• Shu Z, Ai Y, Liu X et al. Astragalus polysaccharide reduces the incidence of postoperative urethral fistula in the rabbit model of hypospadias Research Square 2023-01-27
• Zhou C, Lei F, Mittermaier M et al. Opposing Roles of Blood-Borne Monocytes and Tissue-Resident Macrophages in Limbal Stem Cell Damage after Ocular Injury Cells 2023-08-18 [PMID: 37626899] (Immunohistochemistry)
• Gadanec LK, Andersson U, Apostolopoulos V, Zulli A Glycyrrhizic Acid Inhibits High-Mobility Group Box-1 and Homocysteine-Induced Vascular Dysfunction Nutrients 2023-07-18 [PMID: 37513606]
• Singh J, Thachil T, Eapen M Et Al. Immunohistochemical investigation of cytokine expression levels as biomarkers in transrectal ultrasound-guided needle biopsy specimens of prostate adenocarcinoma Molecular and Clinical Oncology 2021-07-16 [PMID: 34405051] (IHC-P)
• Paschalis EI, Lei F, Zhou C et al. The Role of Microglia and Peripheral Monocytes in Retinal Damage Following Corneal Chemical Injury. Am J Pathol 2018-04-06 [PMID: 29630857] (IF/IHC)
  
  Details:
  This citation used the FITC version of this antibody.
• Cao Q, Zhang J, Gao L et al. Dickkopf-3 upregulation mediates the cardioprotective effects of curcumin on chronic heart failure. Mol Med Rep 2018-05-01 [PMID: 29568962] (IF/IHC, Rabbit)
• Zhou C, Robert MC, Kapoulea V et al. Sustained Subconjunctival Delivery of Infliximab Protects the Cornea and Retina Following Alkali Burn to the Eye. Invest. Ophthalmol. Vis. Sci. 2017-01-01 [PMID: 28114570] (IF/IHC, Rabbit)
  
  Details:
  This citation used the FITC version of this antibody.
• Li J, Das JR, Tang P et al. Transmembrane TNF-alpha Facilitates HIV-1 Infection of Podocytes Cultured from Children with HIV-Associated Nephropathy. J. Am. Soc. Nephrol. 2016-11-03 [PMID: 27811066]
• Azeez IA, Olopade F, Laperchia C et al. Regional Myelin and Axon Damage and Neuroinflammation in the Adult Mouse Brain After Long-Term Postnatal Vanadium Exposure J. Neuropathol. Exp. Neurol. 2016-07-06 [PMID: 27390101] (IF/IHC, Mouse)
• Kishore D, Gopal A, Gambhir I, Diwaker A Aging immunity, immunosenescence, or inflamm-aging: A comparative study of cytokines J Med Soc 2019-10-14 (ELISA, Human)
• Wang Z, Zibrila AI, Liu S et al. Acetylcholine Ameliorated TNF-alpha-induced Primary Trophoblast Malfunction via Muscarinic Receptors Biol. Reprod. 2020-09-09 [PMID: 32902620]
• Mardjiati S, Wulan M, Laswati H, Hadi U, et al. Physical Exercise in Clinical Stage IIhuman Immunodeficiency Virus Infection Patients’ Increasesskeletal Muscle MAss Through the Increasing of Myogenic Regulatory Factors Expression Indian Journal of Forensic Medicine & Toxicology 2021-09-08

Review for TNF-alpha Antibody (NB600-1422) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer IHC-P Other 08/11/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: sheep tonsil
• Species: Other

FAQs for TNF-alpha Antibody (NB600-1422). (Showing 1 - 4 of 4 FAQs).

1. I need to know about TNF-alpha antibody kits for mice.  ..show answer..
   • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.  ..show answer..
   • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?  ..show answer..
   • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?  ..show answer..
   • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Bioinformatics

• Gene Symbol: TNF
• Entrez:
  • 7124(Human)