TNF-alpha Antibody [Unconjugated]

Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for TNF-a at approximately 14 and 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for TNF-a at approximately 14 and 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for TNF-a at approximately 14 and 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TNF-a was detected in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line treated with LPS using Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TNF‑ alpha was detected in immersion fixed activated mouse T Cells using 15 µg/mL Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑410‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

TNF‑ alpha was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Recombinant Mouse TNF-a (410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-a (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA). The ND50 is typically 1.5-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.

TNF‑ alpha was detected in immersion fixed paraffin-embedded sections of Human Spleen using Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

WNT-5A induces a proinflammatory transformation in mouse microglia. (A, B) iNOS, COX-2 and TNF alpha were detected by immunoblotting in lysates from mouse primary microglia after WNT-5A stimulation (ctrl, 300 ng/ml, 6 hours). (B’) shows TNF alpha levels in the supernatant of primary microglia under ctrl conditions and upon WNT-5A stimulation (ctrl, 300 ng/ml, 24 hours; n = 4). At least three experiments are summarized in the bar graphs. Data are normalized to ctrl. Error bars give s.e.m. (C) Microglial proliferation was assessed by an MTT assay monitoring mitochondrial activity, which is proportional to cell number [see Additional file 1: Figure S5]. Stimulation with WNT-5A (300 ng/ml, 24 hours) increased MTT, which was blocked by PTX (100 ng/ml, overnight) or the MEK1/2 (10 μM) inhibitor, SL327. (D). Experiments were done in triplicate and data from three independent experiments are shown. *, P AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu, Mu
• Applications: WB, IHC, B/N, CyTOF-ready, ELISA(Cap), Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TNF-alpha Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse TNF-alpha
  Leu80-Leu235
  Accession # P06804
• Specificity: Detects mouse TNF-alpha in ELISAs. Detects human and mouse TNF-alpha in Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat TNF-alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNF
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 0.1-0.4 ug/mL
  • Western Blot 2 ug/mL
• Application Notes: ELISA Capture:Mouse TNF-alpha Antibody (Catalog # AF-410-NA) ELISA Detection: Mouse TNF-alpha Biotinylated Antibody (Catalog # BAF410) Standard: Recombinant Mouse TNF-alpha aa 80-235 (Catalog # 410-MT)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF-alpha Antibody [Unconjugated]

• APC1 protein
• Cachectin
• Cachetin
• DIF
• TNF
• TNF, monocyte-derived
• TNFA
• TNF-A
• TNFalpha
• TNF-alpha
• TNF-alphacachectin
• TNFATNF, macrophage-derived
• TNFG1F
• TNFSF1A
• TNFSF2
• TNFSF2TNF superfamily, member 2
• tumor necrosis factor (TNF superfamily, member 2)
• tumor necrosis factor alpha
• Tumor necrosis factor ligand superfamily member 2
• tumor necrosis factor
• tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha, TNF- alpha , TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Mouse TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, mouse TNF-alpha shares 94% aa sequence identity with rat and 70%-77% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TNF-alpha Antibody (AF-410-NA)(119)

Publications using AF-410-NA

• Wunderle, V;Wilhelm, T;Boukeileh, S;Go beta en, J;Margreiter, MA;Sakurov, R;Capellmann, S;Schwoerer, M;Ahmed, N;Bronneberg, G;Arock, M;Martin, C;Schubert, T;Levi-Schaffer, F;Rossetti, G;Tirosh, B;Huber, M; KIRA6 is an Effective and Versatile Mast Cell Inhibitor of IgE-mediated Activation European journal of immunology 2024-12-15 [PMID: 39676406] (ELISA Capture, Mouse)
• Zhong, X;Wu, H;Zhang, W;Shi, Y;Gwack, Y;Xue, HH;Sun, Z; Distinct ROR?t-dependent Th17 immune responses are required for autoimmune pathogenesis and protection against bacterial infection Cell reports 2024-11-05 [PMID: 39504243] (Neutralization, Mouse)
• Liu, A;Hayashi, M;Ohsugi, Y;Katagiri, S;Akira, S;Iwata, T;Nakashima, T; The IL-33/ST2 axis is protective against acute inflammation during the course of periodontitis Nature communications 2024-03-28 [PMID: 38548743] (Immunohistochemistry, Mouse)
• Zhou, X;Hao, H;Chen, Y;Cao, W;Zhu, Z;Ni, Y;Liu, Z;Jia, F;Wang, Y;Ji, J;Peng Zhang, ; Covalently grafted human serum albumin coating mitigates the foreign body response against silicone implants in mice Bioactive materials 2024-04-01 [PMID: 38292409] (IHC, Mouse)
• Oppezzo, A;Monney, L;Kilian, H;Slimani, L;Maczkowiak-Chartois, F;Rosselli, F; Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNF? Cell & bioscience 2023-06-24 [PMID: 37355617] (Flow Cytometry, Mouse)
• Sandro Capellmann, Roland Sonntag, Herdit Schüler, Steffen K. Meurer, Lin Gan, Marlies Kauffmann et al. Transformation of primary murine peritoneal mast cells by constitutive KIT activation is accompanied by loss of Cdkn2a/Arf expression Frontiers in Immunology 3/30/2023 [PMID: 37063827]
• Sandro Capellmann, Roland Sonntag, Herdit Schüler, Steffen K. Meurer, Lin Gan, Marlies Kauffmann et al. Transformation of primary murine peritoneal mast cells by constitutive KIT activation is accompanied by loss of Cdkn2a/Arf expression Frontiers in Immunology 2023-03-30 [PMID: 37063827] (ELISA Capture, Mouse)
• Georgios Theocharidis, Sahar Rahmani, Sangmin Lee, Zhuqing Li, Antonio Lobao, Konstantinos Kounas et al. Murine macrophages or their secretome delivered in alginate dressings enhance impaired wound healing in diabetic mice Biomaterials 9/1/2022 [PMID: 35934520]
• Y Hwang, HC Kim, EJ Shin Effect of rottlerin on astrocyte phenotype polarization after trimethyltin insult in the dentate gyrus of mice Journal of Neuroinflammation, 2022-06-11;19(1):142. 2022-06-11 [PMID: 35690821] (Western Blot, Mouse)
• Adrien Picod, Suella Martino, Pascale Cervera, Gregory Manuceau, Marc Arca, Monica Wittner et al. Deregulated JAK3 mediates growth advantage and hemophagocytosis in extranodal nasal-type natural killer/T-cell lymphoma Haematologica 5/5/2022 [PMID: 35511669]
• B Bai, H Li, L Han, Y Mei, C Hu, Q Mei, J Xu, X Liu Molecular mechanism of the TGF?beta/Smad7 signaling pathway in ulcerative colitis Molecular Medicine Reports, 2022-02-09;25(4):. 2022-02-09 [PMID: 35137923] (In Vivo, Mouse)
• Christopher J. Neufeldt, Berati Cerikan, Mirko Cortese, Jamie Frankish, Ji-Young Lee, Agnieszka Plociennikowska et al. SARS-CoV-2 infection induces a pro-inflammatory cytokine response through cGAS-STING and NF-kappa B Communications Biology 1/12/2022 [PMID: 35022513]
• G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin Single cell transcriptomic landscape of diabetic foot ulcers Nature Communications, 2022-01-10;13(1):181. 2022-01-10 [PMID: 35013299] (IHC, Human)
• Y Luo, F Yao, X Hu, Y Li, Y Chen, Z Li, Z Zhu, S Yu, D Tian, L Cheng, M Zheng, J Jing M1 macrophages impair tight junctions between endothelial cells after spinal cord injury Brain research bulletin, 2022-01-04;0(0):. 2022-01-04 [PMID: 34995751] (Neutralization, Mouse)
• Gayoung Kim, Kyeong-Hun Choi, Hangeun Kim, Dae-Kyun Chung Alleviation of LPS-Induced Inflammation and Septic Shock by Lactiplantibacillus plantarum K8 Lysates International Journal of Molecular Sciences 5/31/2021 [PMID: 34072918]
• Vishal Kothari, Jingjing Tang, Yi He, Farah Kramer, Jenny E. Kanter, Karin E. Bornfeldt ADAM17 Boosts Cholesterol Efflux and Downstream Effects of High-Density Lipoprotein on Inflammatory Pathways in Macrophages Arteriosclerosis, Thrombosis, and Vascular Biology 6/1/2021 [PMID: 33882688]
• MS AlQranei, LT Senbanjo, H Aljohani, T Hamza, MA Chellaiah Lipopolysaccharide- TLR-4 Axis regulates Osteoclastogenesis independent of RANKL/RANK signaling BMC immunology, 2021-03-25;22(1):23. 2021-03-25 [PMID: 33765924] (Neutralization, Mouse)
• RS Najjar, NS Akhavan, S Pourafshar, BH Arjmandi, RG Feresin Cornus officinalis var. koreana Kitam polyphenol extract decreases pro-inflammatory markers in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages by reducing Akt phosphorylation Journal of ethnopharmacology, 2020-12-24;0(0):113734. 2020-12-24 [PMID: 33359857] (Western Blot, Mouse)
• Bing-Yi Shao, Lan Wang, Yang Yu, Liang Chen, Ning Gan, Wen-Ming Huang Effects of CD4⁺ T&nbsp;lymphocytes from ovariectomized mice on bone marrow mesenchymal stem cell proliferation and osteogenic differentiation Experimental and Therapeutic Medicine 9/11/2020 [PMID: 32968441]
• Shenbin Liu, Zhi-Fu Wang, Yang-Shuai Su, Russell S. Ray, Xiang-Hong Jing, Yan-Qing Wang et al. Somatotopic Organization and Intensity Dependence in Driving Distinct NPY-Expressing Sympathetic Pathways by Electroacupuncture Neuron 11/1/2020 [PMID: 32791039]
• T Yoshida, NA Das, AJ Carpenter, R Izadpanah, SA Kumar, S Gautam, SB Bender, U Siebenlist, B Chandrasek Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-kappaB/iNOS/NO-dependent cardiomyocyte contractile depression and death Cell. Signal., 2020-06-15;73(0):109690. 2020-06-15 [PMID: 32553549] (Neutralization, Western Blot, Mouse)
• Z Li, C Zhang, JX Du, J Zhao, MT Shi, MW Jin, H Liu Adipocytes promote tumor progression and induce PD-L1 expression via TNF-&amp;alpha/IL-6 signaling Cancer Cell Int., 2020-05-20;20(0):179. 2020-05-20 [PMID: 32477009] (Neutralization, Human, Mouse)
• Y Takagaki, SM Lee, Z Dongqing, M Kitada, K Kanasaki, D Koya Endothelial autophagy deficiency induces IL6 - dependent endothelial mesenchymal transition and organ fibrosis Autophagy, 2020-01-22;0(0):1-10. 2020-01-22 [PMID: 31965901] (Neutralization, Human)
• X Liu, R Tonello, Y Ling, YJ Gao, T Berta Paclitaxel-activated astrocytes produce mechanical allodynia in mice by releasing tumor necrosis factor-alpha and stromal-derived cell factor 1 J Neuroinflammation, 2019-11-10;16(1):209. 2019-11-10 [PMID: 31707979] (In Vivo, Mouse)
• Q Zhou, X Wu, X Wang, Z Yu, T Pan, Z Li, X Chang, Z Jin, J Li, Z Zhu, B Liu, L Su The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-alpha/IL-33/ST2L signaling promotes gastric cancer metastasis Oncogene, 2019-10-28;0(0):. 2019-10-28 [PMID: 31659258] (Cell Culture, Human)
• Y Xu, Y Xu, Y Zhu, H Sun, C Juguilon, F Li, D Fan, L Yin, Y Zhang Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis Mol. Ther., 2019-09-12;0(0):. 2019-09-12 [PMID: 31604677] (Western Blot, Human, Mouse)
• P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells Aging (Albany NY), 2019-09-26;11(18):7402-7415. 2019-09-26 [PMID: 31557129] (Neutralization, Mouse)
• SM Kim, N Neuendorff, DJ Earnest Role of Proinflammatory Cytokines in Feedback Modulation of Circadian Clock Gene Rhythms by Saturated Fatty Acids Sci Rep, 2019-06-20;9(1):8909. 2019-06-20 [PMID: 31222133] (Neutralization, Mouse)
• X Zhang, D He, S Gao, Y Wei, L Wang Aspergillus�fumigatus enhances human NK cell activity by regulating M1 macrophage polarization Mol Med Rep, 2019-06-06;20(2):1241-1249. 2019-06-06 [PMID: 31173233] (Cell Culture, Human)
• OJ Sul, M Rajasekara, HJ Park, JH Suh, HS Choi MicroRNA-29b Enhances Osteoclast Survival by Targeting BCL-2-Modifying Factor after Lipopolysaccharide Stimulation Oxid Med Cell Longev, 2019-04-10;2019(0):6018180. 2019-04-10 [PMID: 31093317] (Western Blot, Mouse)
• J Pido-Lopez, B Tanudjojo, S Farag, MK Bondulich, R Andre, SJ Tabrizi, GP Bates Inhibition of tumour necrosis factor alpha in the R6/2 mouse model of Huntington&#039;s disease by etanercept treatment Sci Rep, 2019-05-10;9(1):7202. 2019-05-10 [PMID: 31076648] (Western Blot, Mouse)
• Laura Evgin, Amanda L. Huff, Timothy Kottke, Jill Thompson, Amy M. Molan, Christopher B. Driscoll et al. Suboptimal T-cell Therapy Drives a Tumor Cell Mutator Phenotype That Promotes Escape from First-Line Treatment Cancer Immunology Research 5/1/2019 [PMID: 30940643]
• Dorian A. Rosen, Scott M. Seki, Anthony Fernández-Castañeda, Rebecca M. Beiter, Jacob D. Eccles, Judith A. Woodfolk et al. Modulation of the Sigma-1 Receptor-IRE1 pathway is beneficial in preclinical models of inflammation and sepsis. Science Translational Medicine 2/6/2019 [PMID: 30728287]
• JA Wixey, KM Lee, SM Miller, K Goasdoue, PB Colditz, S Tracey Bjo, KK Chand Neuropathology in intrauterine growth restricted newborn piglets is associated with glial activation and proinflammatory status in the brain J Neuroinflammation, 2019-01-08;16(1):5. 2019-01-08 [PMID: 30621715] (IHC-P, Porcine)
• C Widmann, C Gandin, A Petit-Pait, M Lazdunski, C Heurteaux The Traditional Chinese Medicine MLC901 inhibits inflammation processes after focal cerebral ischemia Sci Rep, 2018-12-24;8(1):18062. 2018-12-24 [PMID: 30584250] (IHC, Mouse)
• W Sun, N Meednu, A Rosenberg, J Rangel-Mor, V Wang, J Glanzman, T Owen, X Zhou, H Zhang, BF Boyce, JH Anolik, L Xing B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation Nat Commun, 2018-12-03;9(1):5127. 12/3/2018 [PMID: 30510188]
• RF Righetti, TM Dos Santos, LDN Camargo, LRCRB Aristótele, S Fukuzaki, FCR de Souza, FPR Santana, MVR de Agrela, MM Cruz, MIC Alonso-Val, IS Genaro, BM Saraiva-Ro, EA Leick, MA Martins, CM Prado, IFLC Tibério Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice Front Pharmacol, 2018-10-04;9(0):1021. 2018-10-04 [PMID: 30337870] (IHC, Mouse)
• M Kremer, I Yalcin, Y Goumon, X Wurtz, L Nexon, D Daniel, S Megat, RA Ceredig, C Ernst, G Turecki, V Chavant, JF Theroux, A Lacaud, LE Joganah, V Lelievre, D Massotte, PE Lutz, R Gilsbach, E Salvat, M Barrot A dual noradrenergic mechanism for the relief of neuropathic allodynia by the antidepressant drugs duloxetine and amitriptyline J. Neurosci., 2018-09-24;0(0):. 2018-09-24 [PMID: 30249798] (Western Blot, Mouse)
• Xiaowen Shi, Yasuyuki Ohta, Jingwei Shang, Ryuta Morihara, Yumiko Nakano, Yusuke Fukui et al. Neuroprotective effects of SMTP-44D in mice stroke model in relation to neurovascular unit and trophic coupling Journal of Neuroscience Research 12/1/2018 [PMID: 30242877]
• Chunyan Zhu, Qionghong Xu, Zhiyun Mao, Na Lin The Chinese Medicine Wu-Tou Decoction Relieves Neuropathic Pain by Inhibiting Hippocampal Microglia Activation Scientific Reports 8/16/2018 [PMID: 30115941]
• CJ Lee, SJ Moon, JH Jeong, S Lee, MH Lee, SM Yoo, HS Lee, HC Kang, JY Lee, WS Lee, HJ Lee, EK Kim, JY Jhun, ML Cho, JK Min, YY Cho Kaempferol targeting on the fibroblast growth factor receptor 3-ribosomal S6 kinase 2 signaling axis prevents the development of rheumatoid arthritis Cell Death Dis, 2018-03-14;9(3):401. 2018-03-14 [PMID: 29540697] (ELISA Development (Capture), Mouse)
• Subo Yuan, Yuqiang Shi, Kaiwen Guo, Shao-Jun Tang Nucleoside Reverse Transcriptase Inhibitors (NRTIs) Induce Pathological Pain through Wnt5a-Mediated Neuroinflammation in Aging Mice Journal of Neuroimmune Pharmacology 6/1/2018 [PMID: 29429030]
• Subo Yuan, Yuqiang Shi, Kaiwen Guo, Shao-Jun Tang Nucleoside Reverse Transcriptase Inhibitors (NRTIs) Induce Pathological Pain through Wnt5a-Mediated Neuroinflammation in Aging Mice Journal of Neuroimmune Pharmacology 2018-06-01 [PMID: 29429030] (Western Blot, Mouse)
• K Zenke, M Muroi, KI Tanamoto AKT1 distinctively suppresses MyD88-depenedent and TRIF-dependent Toll-like receptor signaling in a kinase activity-independent manner Cell. Signal., 2017-12-11;43(0):32-39. 2017-12-11 [PMID: 29242168] (Western Blot, Mouse)
• Wendy E. Geslewitz, Caroline M. Percopo, Helene F. Rosenberg Eosinophil persistence in vivo and sustained viability ex vivo in response to respiratory challenge with fungal allergens Clinical & Experimental Allergy 1/1/2018 [PMID: 29068539]
• T Kottke, L Evgin, KG Shim, D Rommelfang, N Boisgeraul, S Zaidi, RM Diaz, J Thompson, E Ilett, M Coffey, P Selby, H Pandha, K Harrington, A Melcher, R Vile Subversion of NK-cell and TNF? Immune Surveillance Drives Tumor Recurrence Cancer Immunol Res, 2017-10-15;5(11):1029-1045. 2017-10-15 [PMID: 29038298] (Neutralization, Mouse)
• SH Lee, JY Kwon, SY Kim, K Jung, ML Cho Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis Sci Rep, 2017-08-31;7(1):10133. 2017-08-31 [PMID: 28860618] (ELISA Development (Capture), Mouse)
• Dae-Sun Kim, Himika Dastidar, Chunfen Zhang, Franz J. Zemp, Keith Lau, Matthias Ernst et al. Smac mimetics and oncolytic viruses synergize in driving anticancer T-cell responses through complementary mechanisms Nature Communications 8/24/2017 [PMID: 28839138]
• Y Oh, YS Kwon, BD Jung Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice Int J Med Sci, 2017-07-18;14(8):729-734. 2017-07-18 [PMID: 28824307] (ELISA Development (Capture), Mouse)
• N Matsushita, MT Hassanein, M Martinez-C, R Lazaro, SW French, W Xie, K Lai, M Karin, H Tsukamoto Gender difference in NASH susceptibility: Roles of hepatocyte Ikk? and Sult1e1 PLoS ONE, 2017-08-10;12(8):e0181052. 2017-08-10 [PMID: 28797077] (IHC-P, Mouse)
• Ya-Jun Ren, Lei Zhang, Tao Bai, Hong-Lu Yu, Ying Li, Wei Qian et al. Transfer of CD11c+ lamina propria mononuclear phagocytes from post-infectious irritable bowel syndrome causes mucosal barrier dysfunction and visceral hypersensitivity in recipient mice International Journal of Molecular Medicine 6/1/2017 [PMID: 28440501]
• KJ Chung, A Chatzigeor, M Economopou, R Garcia-Mar, VI Alexaki, I Mitroulis, M Nati, J Gebler, T Ziemssen, SE Goelz, J Phieler, JH Lim, KP Karalis, T Papayannop, M Blüher, G Hajishenga, T Chavakis A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity Nat. Immunol., 2017-04-17;0(0):. 2017-04-17 [PMID: 28414311] (Neutralization, Mouse)
• T Tsunekawa, R Banno, A Mizoguchi, M Sugiyama, T Tominaga, T Onoue, D Hagiwara, Y Ito, S Iwama, M Goto, H Suga, Y Sugimura, H Arima Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in Microglia EBioMedicine, 2017-01-09;0(0):. 2017-01-09 [PMID: 28094236] (IHC-Fr, Mouse)
• C Dempsey Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-? and cognitive function in APP/PS1 mice Brain Behav. Immun, 2016-12-18;0(0):. 2016-12-18 [PMID: 28003153] (ELISA Development (Capture), Mouse)
• M C Muñoz Chronic blockade of the AT2 receptor with PD123319 impairs insulin signaling in C57BL/6 mice Peptides, 2016-12-12;0(0):. 2016-12-12 [PMID: 27979738] (Western Blot, Mouse)
• Yoonsook Kim Oryza sativa (Rice) Hull Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages by Suppressing Extracellular Signal-regulated Kinase, c-Jun N-terminal Kinase, and Nuclear Factor-?B Activation Pharmacogn Mag, 2016-10-01;12(48):295-301. 2016-10-01 [PMID: 27867272] (ELISA Development (Capture), Mouse)
• Seth T. Eisenman, Simon J. Gibbons, Pieter-Jan Verhulst, Gianluca Cipriani, Dieter Saur, Gianrico Farrugia Tumor necrosis factor alpha derived from classically activated “M1” macrophages reduces interstitial cell of Cajal numbers Neurogastroenterology & Motility 4/1/2017 [PMID: 27781339]
• Tzu-Ying Chuang, Yong Guo, Scott M. Seki, Abagail M. Rosen, David M. Johanson, James W. Mandell et al. LRP1 expression in microglia is protective during CNS autoimmunity Acta Neuropathologica Communications 12/1/2016 [PMID: 27400748]
• Xu X, Meng Q, Erben U et al. Myeloid-derived suppressor cells promote B-cell production of IgA in a TNFR2-dependent manner. Cell. Mol. Immunol. 5/2/2016 [PMID: 27133471]
• SY Lim, AE Yuzhalin, AN Gordon-Wee, RJ Muschel Tumor-infiltrating monocytes/macrophages promote tumor invasion and migration by upregulating S100A8 and S100A9 expression in cancer cells Oncogene, 2016-04-18;0(0):. 2016-04-18 [PMID: 27086923] (Neutralization, Mouse)
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FAQs for TNF-alpha Antibody (AF-410-NA). (Showing 1 - 4 of 4 FAQs).

1. I need to know about TNF-alpha antibody kits for mice.  ..show answer..
   • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.  ..show answer..
   • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?  ..show answer..
   • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?  ..show answer..
   • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Bioinformatics

• Gene Symbol: TNF
• Uniprot:
  • P06804()