Mouse TNF-alpha ELISA Kit (Colorimetric)

ELISA: Mouse TNF-alpha ELISA Kit (Colorimetric) [NBP2-31085] - Standard Curve

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Cell Culture Supernatants, Plasma (EDTA, citrate), buffered solution, Serum
• Standard Curve Range: 31.3 - 2000 pg/mL
• Sensitivity: 3.7 pg/ml

Mouse TNF-alpha ELISA Kit (Colorimetric) Summary

• Description: The mouse TNF-alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse TNF-alpha.
• Specificity: The assay detects both natural and recombinant mouse TNF alpha. The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into mouse IL-10 positive serum. There was no crossreactivity detected.
• Standard Curve Range: 31.3 - 2000 pg/mL
• Sensitivity: 3.7 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 5.7%
• Intra-Assay: CV% < 6.5%
• Spike Recovery: Please See Protocol for Specific Spike Recovery Details
• Sample Volume: 50 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TNF

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse TNF-alpha ELISA Kit (Colorimetric)

• APC1 protein
• Cachectin
• Cachetin
• DIF
• TNF
• TNF, monocyte-derived
• TNFA
• TNF-A
• TNFalpha
• TNF-alpha
• TNF-alphacachectin
• TNFATNF, macrophage-derived
• TNFG1F
• TNFSF1A
• TNFSF2
• TNFSF2TNF superfamily, member 2
• tumor necrosis factor (TNF superfamily, member 2)
• tumor necrosis factor alpha
• Tumor necrosis factor ligand superfamily member 2
• tumor necrosis factor
• tumor necrosis factor-alpha

Background

Tumor necrosis factor (TNF)-alpha is a pro-inflammatory cytokine belonging to the TNF superfamily that is secreted by monocytes/macrophages, T cell, and natural killer (NK), among others (1). TNF-alpha is synthesized as a 233 amino acid (aa) transmembrane protein (mTNF-alpha) with a theoretical molecular weight (MW) of 26 kDa (1,2) that forms a homotrimer. mTNF-alpha is cleaved by TNF-alpha converting enzyme (TACE) and released in its 157 aa, 17 kDa soluble form (sTNF-alpha) (1-5). Both mTNF-alpha and sTNF-alpha are capable of binding type 1 TNF receptors (TNFR1), whereas mTNF-alpha predominately binds to TNFR2 (1,2). TNF-alpha binding to its receptors causes receptor recruitment of adaptor proteins, formation of signaling complexes, and downstream signaling cascades (e.g. MAPK, NF-kappaB, and Caspase-8), leading to distinct cellular responses such as survival, proliferation, inflammation, necroptosis, and apoptosis (1-5).

TNF-alpha is critical for normal immune response; however, dysregulation of TNF-alpha production can result in various pathologies (2,4,5). Excessive production of pro-inflammatory cytokines including interleukin 1 (IL-1), IL-6, and TNF-alpha has been implicated in an array of autoimmune diseases like rheumatoid arthritis (RA), inflammatory bowel disease (IBD), and psoriasis (2,4,5). Anti-TNF monoclonal antibodies, including Infliximab, and soluble TNFR have been approved for the treatment of autoimmune and TNF-mediated diseases (5). Additionally, data suggests that TNF inhibitors can be beneficial for treating patients experiencing immune-related adverse events associated with immune checkpoint inhibitor cancer treatment (6).

References

1. Holbrook J, Lara-Reyna S, Jarosz-Griffiths H, McDermott M. Tumour necrosis factor signalling in health and disease. F1000Res. 2019;8:F1000 Faculty Rev-111. https://doi.org/10.12688/f1000research.17023.1

2. Jang DI, Lee AH, Shin HY, et al. The Role of Tumor Necrosis Factor Alpha (TNF-alpha) in Autoimmune Disease and Current TNF-alpha Inhibitors in Therapeutics. Int J Mol Sci. 2021;22(5):2719. https://doi.org/10.3390/ijms22052719

3. Horiuchi T, Mitoma H, Harashima S, Tsukamoto H, Shimoda T. Transmembrane TNF-alpha: structure, function and interaction with anti-TNF agents. Rheumatology (Oxford). 2010;49(7):1215-1228. https://doi.org/10.1093/rheumatology/keq031

4. Webster JD, Vucic D. The Balance of TNF Mediated Pathways Regulates Inflammatory Cell Death Signaling in Healthy and Diseased Tissues. Front Cell Dev Biol. 2020;8:365. https://doi.org/10.3389/fcell.2020.00365

5. Kalliolias GD, Ivashkiv LB. TNF biology, pathogenic mechanisms and emerging therapeutic strategies. Nat Rev Rheumatol. 2016; 12(1):49-62. https://doi.org/10.1038/nrrheum.2015.169

6. Chen AY, Wolchok JD, Bass AR. TNF in the era of immune checkpoint inhibitors: friend or foe?. Nat Rev Rheumatol. 2021;17(4):213-223. doi:10.1038/s41584-021-00584-4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for TNF-alpha ELISA Kit (NBP2-31085)(3)

Publications using NBP2-31085

• Can Wei, Meixin Shi, Shiyun Dong, Zhitao Li, Bingbing Zhao, Dan Liu, Guopeng Li, Jie Cen, Ligen Yu, Xiao Liang, Lili Shi SIRT5-related lysine demalonylation of GSTP1 contributes to cardiomyocyte pyroptosis suppression in diabetic cardiomyopathy International Journal of Biological Sciences 2024-01-01 [PMID: 38169591]
• Luan G, Pan F, Bu L Et Al. Butorphanol Promotes Macrophage Phenotypic Transition to Inhibit Inflammatory Lung Injury via kappa Receptors Frontiers in immunology 2021-07-07 [PMID: 34305926] (ELISA)
• Wang J, Zhao L, Li Y et al. HuR induces inflammatory responses in HUVECs and murine sepsis via binding to HMGB1. Mol Med Rep 2018-01-01 [PMID: 29115544] (ELISA, Mouse)

FAQs for TNF-alpha ELISA Kit (NBP2-31085). (Showing 1 - 5 of 5 FAQs).

1. I need to know about TNF-alpha antibody kits for mice.
   • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
   • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
   • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
   • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.
5. How much blood do we need for Mouse TNF-alpha ELISA Kit (Colorimetric) assay?because we do not want to kill our mice.
   • You will need ~50ul. Please see the manual for more details

Bioinformatics

• Gene Symbol: TNF