TOP2A Antibody

Western Blot: TOP2A Antibody [NB110-5829] - Analysis of Native Human Raji Cells.

Western Blot: TOP2A Antibody [NB110-5829] - Analysis of Raji Cells.

Product Discontinued

TOP2A Antibody Summary

• Immunogen: A synthetic peptide, corresponding to aa 1513-1530 (RAKKPIKYLEESDEDDLF) from the C-terminus of human topoisomerase IIalpha
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TOP2A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 10 ug/ml
  • Western Blot 5 - 10 ug/mL
• Control:

  Raji Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TOP2A Antibody

• DNA topoisomerase (ATP-hydrolyzing)
• DNA topoisomerase 2-alpha
• DNA topoisomerase II, 170 kD
• DNA topoisomerase II, alpha isozyme
• EC 5.99.1.3
• TOP2
• TOP2A
• TOP2DNA gyrase
• topoisomerase (DNA) II alpha (170kD)
• topoisomerase (DNA) II alpha 170kDa
• TP2A

Background

Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Note that Ki67/M1B1 is also present in G1-phase. Topo IIa is also implicated in drug resistance of tumor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TOP2A
• Entrez:
  • 7153(Human)
• Uniprot:
  • P11388()