TOR/mTOR Antibody - BSA Free

Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: TOR/mTOR Antibody [NB100-240] - Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-mTOR antibody NB100-240 used for IP at 3 ug per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody NB100-241. For blotting immunoprecipitated mTOR, NB100-240 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Rt, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TOR/mTOR Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human Mammalian Target of Rapamycin using the numbering given in entry NP_004949.1 (GeneID 2475).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MTOR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:100-1:2000
• Application Notes: Use in Immunohistochemistry-paraffin reported in scientific literature (Suzuki H et al).
• Control:

  TOR/mTOR Overexpression Lysate

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Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29728793).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TOR/mTOR Antibody - BSA Free

• EC 2.7.11.1
• FK506 binding protein 12-rapamycin associated protein 1
• FK506 binding protein 12-rapamycin associated protein 2
• FK506-binding protein 12-rapamycin complex-associated protein 1
• FKBP12-rapamycin complex-associated protein
• FLJ44809
• FRAP
• FRAP1
• FRAP1FKBP12-rapamycin complex-associated protein 1
• FRAP2
• Mammalian target of rapamycin
• mechanistic target of rapamycin (serine/threonine kinase)
• Mechanistic target of rapamycin
• MTOR
• RAFT1
• rapamycin and FKBP12 target 1
• rapamycin associated protein FRAP2
• Rapamycin target protein 1
• RAPT1FKBP-rapamycin associated protein
• serine/threonine-protein kinase mTOR
• TOR

Background

FKBP 12 rapamycin associated protein (FRAP) is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 235 kDa protein (symbolized RAFT 1) with significant homology to the Saccharomyces cerevisiae protein TOR 1 and has been shown to associate with the immunophilin FKBP 12 in a rapamycin-dependent fashion. The FKBP 12 rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP 12 rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TOR/mTOR Antibody (NB100-240)(11)

Publications using NB100-240

• Sikora M, ?mieszek A, Pielok A, Marycz K MiR-21-5p regulates the dynamic of mitochondria network and rejuvenates the senile phenotype of bone marrow stromal cells (BMSCs) isolated from osteoporotic SAM/P6 mice Stem cell research & therapy 2023-03-29 [PMID: 36978118] (ICC/IF, Mouse)
  
  Details:
  Dilution used in ICC/IF 1:1000
• Foley K, Hewes A, Garceau D et al. The APOE epsilon 3/epsilon 4 Genotype Drives Distinct Gene Signatures in the Cortex of Young Mice Front Aging Neurosci 2022-04-04 [PMID: 35370604]
• A Kowalczuk, N Bourebaba, J Panchuk, K Marycz, L Bourebaba Calystegines Improve the Metabolic Activity of Human Adipose Derived Stromal Stem Cells (ASCs) under Hyperglycaemic Condition through the Reduction of Oxidative/ER Stress, Inflammation, and the Promotion of the AKT/PI3K/mTOR Pathway Biomolecules, 2022-03-16;12(3):. 2022-03-16 [PMID: 35327652]
• Xie B, Zhao M, Song D et Al. Induction of autophagy and suppression of type I IFN secretion by CSFV Autophagy 2020-03-21 [PMID: 32160078]
• Puigoriol-Illamola D, Martinez-Damas M, Grinan-Ferre C, Pallas M Chronic Mild Stress Modified Epigenetic Mechanisms Leading to Accelerated Senescence and Impaired Cognitive Performance in Mice Preprints 2020-01-29 [PMID: 32050516] (WB, Mouse)
• Lisi L, Ciotti GMP, Chiavari M et al. Phospho-mTOR expression in human glioblastoma microglia-macrophage cells Neurochem. Int. 2019-06-10 [PMID: 31195027] (WB, Human)
• Guo Y, Wang F, Li H et al. Metformin Protects Against Spinal Cord Injury by Regulating Autophagy via the mTOR Signaling Pathway. Neurochem Res 2018-05-04 [PMID: 29728793] (Rat)
• Suzuki H, Roa JC, Kawamoto T et al. Expression of insulin-like growth factor I receptor as a biomarker for predicting prognosis in biliary tract cancer patients. Mol Clin Oncol 2015-05-01 [PMID: 26137252] (IF/IHC)
• Liu DC, Yang ZL. MTDH and EphA7 are markers for metastasis and poor prognosis of gallbladder adenocarcinoma. Diagn Cytopathol. 2011-09-30 [PMID: 21964981] (IF/IHC, Human)
• Lionello M, Blandamura S, Loreggian L, Ottaviano G, Giacomelli L, Marchese-Ragona R, Velardita C, Staffieri A, Marioni G. High mTOR expression is associated with a worse oncological outcome in laryngeal carcinoma treated with postoperative radiotherapy: a pilot study. J Oral Pathol Med;41(2):136-40. doi: 10.1111/j.1600-0714.2011.01083.x. 2012-02-01 [PMID: 21943204]
• Bolster DR, Vary TC, Kimball SR et al. Leucine regulates translation initiation in rat skeletal muscle via enhanced eIF4G phosphorylation. J Nutr 2004-07-01 [PMID: 15226457]

Bioinformatics

• Gene Symbol: MTOR