TORC1 Antibody - BSA Free

Western Blot: TORC1 Antibody [NBP1-77940] - Western blot using affinity purified TORC1 antibody shows detection of enCanineenous TORC1 (lower arrowhead) and TORC1-MAML2 fusion protein (top arrowhead) in cell lysates. Lane 1 contains lysate from cells expressing TORC1 only. Lane 2 contains lysate from cells transfected with fusion protein. Lane 3 contains lysate from cells strongly expressing TORC1 and weakly expressing the fusion protein. Lane 4 contains lysate from control cells. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000.

ELISA: TORC1 Antibody [NBP1-77940] - ELISA Results of TORC1 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The working dilution is 1: 19,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate stabilizer, Goat Anti-Rabbit HRP conjugated and TMB substrate.

Western blot using affinity purified anti-MECT1 antibody shows detection of endogenous MECT1 (lower arrowhead) and MECT1-MAML2 fusion protein (top arrowhead) in cell lysates. Lane 1 contains lysate from cells expressing MECT1 only. Lane 2 contains lysate from cells transfected with fusion protein. Lane 3 contains lysate from cells strongly expressing MECT1 and weakly expressing the fusion protein. Lane 4 contains lysate from control cells. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Frederick Kaye, CCR-NCI, Bethesda, MD.

• Reactivity: Hu, Mu, Rt, Bv, PF, Ze
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TORC1 Antibody - BSA Free Summary

• Description: This affinity-purified antibody is directed against human TORC1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified TORC1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 19-34 of human TORC1 protein. (Uniprot: Q6UUV9)
• Specificity: This affinity-purified antibody is directed against human TORC1 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRTC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:40000
  • Western Blot 1:500-1:2000
• Application Notes: This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80-95 kDa in size corresponding to MECT1 protein by western blotting in the appropriate cell lysate or extract. This antibody is will react with isoform 1 and 2 of MECT1.

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with TORC1 protein from human, mouse, rat, zebrafish, pufferfish and bovine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TORC1 Antibody - BSA Free

• CREB regulated transcription coactivator 1
• CRTC1
• FLJ14027WAMTP1
• KIAA0616Transducer of regulated cAMP response element-binding protein 1
• MECT1
• MECT1CREB-regulated transcription coactivator 1
• mucoepidermoid carcinoma translocated 1
• Mucoepidermoid carcinoma translocated protein 1
• TORC1
• TORC1TORC-1
• Transducer of CREB protein 1
• transducer of regulated cAMP response element-binding protein (CREB) 1
• WAMTP1

Background

MECT1 (also known as MucoEpidermoid Carcinoma Translocated 1, Transducer of regulated cAMP response element-binding protein 1, TORC1, and Transducer of CREB protein 1) is a nuclear protein that functions as a transcriptional coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites. MECT1does not appear to modulate CREB1 DNA-binding activity but

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRTC1
• Uniprot:
  • Q6UUV9(Human)
  • Q9H8V3(Human)