TRIM32 Antibody

Western Blot: TRIM32 Antibody [NBP1-33737] - A. 30 ug 293T whole cell lysate/extract 7.5 % SDS-PAGETRIM32 antibody dilution: 1:1000

Immunocytochemistry/Immunofluorescence: TRIM32 Antibody [NBP1-33737] - A375 cells were fixed in ice-cold MeOH for 5 min. Green: TRIM32 protein stained by TRIM32 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: TRIM32 Antibody [NBP1-33737] - Mouse brain diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: TRIM32 Antibody [NBP1-33737] - A. 5 ug 293T whole cell lysate/extract B. 5 ug whole cell lysate/extract of DDDDK-human TRIM32-transfected 293T cells 7.5 % SDS-PAGE TRIM32 antibody dilution: 1:20000

• Reactivity: Hu, Mu, Rt, Fe, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TRIM32 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human TRIM32. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (98%), Rhesus Macaque (99%), Feline (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM32
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:20000
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Cat (98%), Bovine (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TRIM32 Antibody

• 72 kDa Tat-interacting protein
• BBS11
• BBS1172-KD
• EC 6.3.2.-
• HT2A
• HT2Alimb girdle muscular dystrophy 2H (autosomal recessive)
• LGMD2H
• TATIP
• TRIM32
• tripartite motif containing 32
• Zinc finger protein HT2A
• zinc-finger protein HT2A

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TRIM32 Antibody (NBP1-33737)(3)

Publications using NBP1-33737

• Servian-Morilla E, Cabrera-Serrano M, Rivas-Infante E et al. Altered myogenesis and premature senescence underlie human TRIM32-related myopathy Acta Neuropathol Commun 2019-03-01 [PMID: 30823891] (WB, IF/IHC, Human)
• Assereto S, Piccirillo R, Baratto S et al. The ubiquitin ligase tripartite-motif-protein 32 is induced in Duchenne muscular dystrophy. Lab Invest 2016-06-13 [PMID: 27295345] (WB, Mouse)
• Fatima M, Kumari R, Schwamborn JC et al. Tripartite containing motif 32 modulates proliferation of human neural precursor cells in HIV-1 neurodegeneration. Cell Death Differ. 2015-11-20 [PMID: 26586575] (IHC-P, Human)

Bioinformatics

• Gene Symbol: TRIM32