TRIP1 Antibody - BSA Free

Western Blot: TRIP1 Antibody [NBP2-16300] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: TRIP1 Antibody [NBP2-16300] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Rb
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRIP1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human TRIP1. The exact sequence is proprietary.
• Predicted Species: Mouse (98%), Rat (98%), Porcine (98%), Bovine (98%), Rabbit (96%), Chicken (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF3I
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 40% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Protein A purified

Alternate Names for TRIP1 Antibody - BSA Free

• eIF-3-beta
• eIF3-beta
• eIF3i
• eIF3-p36
• EIF3S2TGF-beta receptor-interacting protein 1
• Eukaryotic translation initiation factor 3 subunit 2
• eukaryotic translation initiation factor 3 subunit I
• eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD)
• eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa
• eukaryotic translation initiation factor 3, subunit I
• predicted protein of HQ2242
• TGFbeta receptor-interacting protein 1
• TRIP-1
• TRIP-1eIF3 p36
• TRIP1PRO2242

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: EIF3I
• Uniprot:
  • Q13347(Human)