TRPM7 Antibody

Western Blot: TRPM7 Antibody [NBP3-23362] - Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) NBP3-23362 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TRPM7 Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: TRPM7 synthetic peptide (coupled to carrier) corresponding to amino acids from the cytoplasmic region of human TRPM7. This site is not well conserved in rat and mouse TRPM7, and has no homology to other TRPM family members. [UniProt# Q96QT4]
• Specificity: This antibody detects a 220 kDa protein on SDS-PAGE immunoblots of human MDA-MB-231 cells and detects a 70 kDa recombinant C-terminal kinase domain of human TRPM7.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRPM7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for TRPM7 Antibody

• CHAK
• CHAK1FLJ25718
• Channel-kinase 1
• EC 2.7.11.1
• Long transient receptor potential channel 7
• LTRPC ion channel family member 7
• LTrpC7
• LTrpC-7
• LTRPC7FLJ20117
• transient receptor potential cation channel subfamily M member 7
• transient receptor potential cation channel, subfamily M, member 7
• transient receptor potential-phospholipase C-interacting kinase
• TRP-PLIK

Background

The TRPM7 (Transient Receptor Protein-PhosphoLipase C-Interacting Kinase) protein is a member of the ion channel proteins. It regulates the calcium concentration in cells, which is imperative for such functions as muscle contractions and neuronal firing. TRPM7, however, is unique among the TRP channel proteins because it is bi-functional. It not only controls ion channels but it also acts as a kinase by phosphorylating proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRPM7