TRPV4 Antibody

Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960] - Rat kidney using Rabbit antibody to TRPV4, dilution 1: 1000, incubated 30 min at RT (using Autostainer).

Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960] - Rat kidney using Rabbit antibody to TRPV4, dilution 1: 1000, incubated 30 min at RT (using Autostainer)

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

TRPV4 Antibody Summary

• Immunogen: A synthetic peptide from amino acid region 300-400 of mouse TRPV4 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRPV4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:1000-1:2000
  • Immunohistochemistry-Paraffin 1: 1000 - 1: 2000
  • Western Blot 1:1000-1:2000
• Application Notes: ICC/IF, IHC reactivity reported in (PMID: 22761937).
• Theoretical MW: 98 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Marmoset (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for TRPV4 Antibody

• CMT2Cosmosensitive transient receptor potential channel 4
• HMSN2C
• Osm-9-like TRP channel 4
• OTRPC4
• OTRPC4SSQTL1
• transient receptor potential cation channel subfamily V member 4
• transient receptor potential cation channel, subfamily V, member 4
• Transient receptor potential protein 12
• TRP12
• TRP12OSM9-like transient receptor potential channel 4
• TRPV4
• Vanilloid receptor-like channel 2
• Vanilloid receptor-like protein 2
• vanilloid receptor-related osmotically activated channel
• Vanilloid receptor-related osmotically-activated channel
• VRL2
• VRL-2
• VROAC
• VR-OAC
• VROACSPSMA

Background

TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure. FUNCTION: Non-selective calcium permanent cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia. SIMILARITY: Belongs to the transient receptor family, TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRPV4 Antibody (NB110-74960)(6)

Publications using NB110-74960

• Yahya F, Mohd Bakri M, Hossain MZ et al. Combination Treatment of TRPV4 Agonist with Cisplatin Promotes Vessel Normalization in an Animal Model of Oral Squamous Cell Carcinoma Medicina (Kaunas) 2022-09-06 [PMID: 36143906] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Rat)
• Alencar GF, Owsiany KM, Karnewar S et Al. Stem Cell Pluripotency Genes Klf4 and Oct4 Regulate Complex SMC Phenotypic Changes Critical in Late-Stage Atherosclerotic Lesion Pathogenesis Circulation 2020-07-17 [PMID: 32674599]
• Wheeler MA, Smith CJ, Ottolini M et al. Genetically targeted magnetic control of the nervous system. Nat Neurosci 2016-05-01 [PMID: 26950006]
• Singh V, Ram M, Kandasamy K et al. Molecular and functional characterization of TRPV4 channels in pregnant and nonpregnant mouse uteri. Life Sci. 2014-12-19 [PMID: 25529150] (WB, Mouse)
  
  Details:
  TRPV4 antibody used for WB to demonstrate the presence of TRPV4 protein in late pregnant and nonpregnant mouse uterus.
• Lunov O, Zablotskii V, Syrovets T et al. WITHDRAWN: Static High-Gradient Magnetic Fields Activate Transient Receptor Potential Vanilloid 4 (TRPV4) Ion Channels Enabling Remote Control of Cell Function. J Biol Chem 2013-06-24 [PMID: 23760272]
• Butenko O, Dzamba D, Benesova J et al. The Increased Activity of TRPV4 Channel in the Astrocytes of the Adult Rat Hippocampus after Cerebral Hypoxia/Ischemia PLoS One 2012-01-01 [PMID: 22761937] (ICC/IF, IF/IHC, Rat)

FAQs for TRPV4 Antibody (NB110-74960). (Showing 1 - 1 of 1 FAQs).

1. We have a customer who is looking for an antibody that is suitable to detect TRPV4/Vanilloid receptor-like protein 2 in sheep FFPE tissues. Could you allign the immunizing peptid of NB110-74960 and NB100-98910 against the sheep sequence (if you have it - I could not find the sheep TRPV4 sequence in UniProt and also, the customer did not supply the sequence) and inform us on the homology between the peptids used to generate NB110-74960/NB100-98910 and the sheep sequence?
   • Sheep and Human TRPV4 are over 90% similar, so most antibodies to the human sequence will be predicted to cross-react. NB110-74960 is probably the best choice for this.

Bioinformatics

• Gene Symbol: TRPV4
• Entrez:
  • 59341(Human)
  • 63873(Mouse)
  • 66026(Rat)
• Uniprot:
  • Q9EPK8(Mouse)