TSG101 Antibody (4A10)

Western Blot: TSG101 Antibody (4A10) [NB200-112] - Whole cell lysates (WCL) or exosome sample from MDA-MB-231 cells was loaded with 10 ug/lane. 10% SDS-PAGE. TSG101 Antibody (NB200-112) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.

Western Blot: TSG101 Antibody (4A10) [NB200-112] - Whole cell lysates (WCL) or exosome sample from MDA-MB-231 cells was loaded with 10 ug/lane. 10% SDS-PAGE. TSG101 Antibody (NB200-112) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.

Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - Human ovarian cancer. TSG101 stained by TSG101 antibody [4A10] (NB200-112).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Flow Cytometry: TSG101 Antibody (4A10) [NB200-112] - TSG101 antibody [4A10] (NB200-112) detects TSG101 protein by flow cytometry analysis.Sample: THP-1 cell.Black: Unlabelled sample was used as a control.Red: TSG101 antibody [4A10] (NB200-112).Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Western Blot: TSG101 Antibody (4A10) [NB200-112] - A. 30 ug NIH-3T3 whole cell lysate/extract. B. 30 ug JC whole cell lysate/extract. C. 30 ug BCL-1 whole cell lysate/extract.

Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Western Blot: TSG101 Antibody (4A10) [NB200-112] - Lane 1: MWM, Lane 2: TSG101 in RAW 264.7 Whole Cell Lysate, Lane 3: Exosome Lysate. Used primary antibody at 1:500 and Donkey Anti-Mouse at 0.5 ug/mL (this could be titrated down to reduce background). Used picogram sensitivity ECL reagents. Image from verified customer review.

Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Western Blot: TSG101 Antibody (4A10) [NB200-112] - Characterization of exosomes. Western blot analysis of exosomes revealed differential expression of TSG101 between HCT116-R exosomes and HCT116-P exosomes. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-53063-y), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - Human breast carcinoma. TSG101 stained by TSG101 antibody [4A10] (NB200-112).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ELISA: TSG101 Antibody (4A10) [NB200-112] - ELISA detection of TSG101 for capture at a concentration of 5 ug/mL and TSG101 for detection at a concentration of 1.5 ug/mL.

Knockdown Validated: TSG101 Antibody (4A10) [NB200-112] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500.

Western Blot: TSG101 Antibody (4A10) [NB200-112] - Characterization of pECs-EVs from different women in secretory & proliferative phase of cycle. Representative TEM images of pECs-EVs from secretory (A,B) & proliferative phase (C,D). Scale bars: 50 nm (A,C); 100 nm (B,D). Representative nanoparticle tracking analysis plots of pECs-EVs from secretory (E) & proliferative phase (F). Western blot showing the presence of different canonical EV markers (CD63, CD9, Alix & TSG101) in secretory & proliferative phase pECs-EVs (n = 4) (G). Negative EV markers used were Calnexin (ER), beta -tubulin & the cytosolic form of DCXR (H). The same markers were evaluated in pECs protein extract as controls for EV enrichment of these markers. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483153), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TSG101 Antibody (4A10) [NB200-112] - Characterization of exosomes. (A) Representative TEM images of exosomes at 22000x magnification (Left) HCT116-R exosomes & (Right) HCT116-P exosomes. (B) Enlarged planar view of a cup-shaped structure for single exosome in our samples at 87000x magnification. (C) Western blot analysis of exosomes revealed differential expression of TSG101, CD9, & CD63 between HCT116-R exosomes & HCT116-P exosomes. (D) Average size distribution & zeta potential of exosomes measured in Zetasizer. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31712601), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TSG101 Antibody (4A10) [NB200-112] - EPS15 depletion affects late endosomal maturation.(A) Co-depletion of SPOPL an EPS15 has an additive effect on influenza A virus infection. (B) EPS15 depletion stabilizes ESCRT components HRS, STAM & TSG101. (C) EPS15 depletion affects LDL uptake in cells resulting in an accumulation of LDL in enlarged vacuoles (upper panel). Late endosomes, visualized by life-cell microscopy of GFP-RAB7, are enlarged in cells depleted of EPS15 (lower panel).DOI://dx.doi.org/10.7554/eLife.13841.016 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/13841), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.TSG101 stained by TSG101 antibody [4A10] (NB200-112) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (NB200-112) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (NB200-112) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.

Product Discontinued

TSG101 Antibody (4A10) Summary

• Immunogen: Amino acids 167-374 of TSG101 protein expressed in E. coli.
• Marker: Exosome Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TSG101
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy 1:10
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:25-1:200
  • Immunocytochemistry/ Immunofluorescence 1:500-1:1000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34576126). Primate reactivity reported in scientific literature (PMID: 18267010). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for TSG101 Antibody (4A10)

• ESCRT-I complex subunit TSG101
• TSG10
• TSG101
• tumor susceptibility gene 10
• tumor susceptibility gene 101 protein
• tumor susceptibility gene 101
• tumor susceptibility protein
• VPS23

Background

Tumor susceptibility 101 protein (human TSG101 theoretical molecular weight 44kDa) is the mammalian homologue of the yeast protein Vps23 which plays a role in endosomal and multivesicular body trafficking. TSG101 forms part of the endosomal sorting complexes required for transport complex 1 (ESCRT-I), a cytosolic multiprotein complex consisting additionally of Vps28, Vps37 (A-D) and Mvb12 (A, B) or UBAP1 (1). TSG101 contains multiple domains including the amino terminal ubiquitin e2 variant (UEV) domain, a proline-rich (RRR) domain, a coiled coil (CC) domain, and a carboxy terminal alpha-helical/steadiness box (SB) domain (2). As part of the ESCRT-I complex, TSG101 interacts through its UEV domain with ubiquitinated membrane proteins and members of the ESCRT-0 complex. The UEV domain plays a critical role for various TSG101 functions such as protein sorting into multivesicular bodies and late endosomes, and in the process of viral budding. For example, TSG101 interacts with the intermediate-conductance, Ca2+ -activated K+ channel (KCa3.1), facilitating its targeting to the lysosome for degradation (3). Additionally, TSG101 has been implicated in the turnover of connexins such as connexins 43 and 45 (4). TSG101 plays a role in other cellular functions including transcriptional regulation, cytokinesis and cell growth (2).

Upon its initial discovery, TSG101 was recognized as a tumor suppressor protein due to the identification of deletions within the TSG101 gene in human breast carcinomas. However, re-examination of the initial findings argued against this function and supported that TSG101 promotes tumorigenesis (2). In agreement with this role, TSG101 expression is upregulated in several types of cancer including breast, ovarian, and colorectal carcinoma.

References

1. Schmidt, O., & Teis, D. (2012). The ESCRT machinery. Current Biology. https://doi.org/10.1016/j.cub.2012.01.028

2. Jiang, Y., Ou, Y., & Cheng, X. (2013). Role of TSG101 in cancer. Frontiers in Bioscience. https://doi.org/10.2741/4099

3. Balut, C. M., Gao, Y., Murray, S. A., Thibodeau, P. H., & Devor, D. C. (2010). ESCRT-dependent targeting of plasma membrane localized KCa3.1 to the lysosomes. American Journal of Physiology - Cell Physiology. https://doi.org/10.1152/ajpcell.00120.2010

4. Su, V., & Lau, A. F. (2014). Connexins: Mechanisms regulating protein levels and intercellular communication. FEBS Letters. https://doi.org/10.1016/j.febslet.2014.01.013

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSG101 Antibody (NB200-112)(100)

Publications using NB200-112

• Kim T, Choodinatha HK, Kim KS et al. Understanding the role of soluble proteins and exosomes in non-invasive urine-based diagnosis of preeclampsia Scientific Reports 2024-10-15 [PMID: 39406891]
• Mut M, Adiguzel Z, Cakir-Aktas C et al. Extracellular-Vesicle-Based Cancer Panels Diagnose Glioblastomas with High Sensitivity and Specificity Cancers 2023-07-26 [PMID: 37568598] (WB, Human)
• Zuppone S, Zarovni N, Vago R The cell type dependent sorting of CD9- and CD81 to extracellular vesicles can be exploited to convey tumor sensitive cargo to target cells Drug delivery 2023-12-01 [PMID: 36579638] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000
• Zuppone S, Zarovni N, Noguchi K et al. Novel loading protocol combines highly efficient encapsulation of exogenous therapeutic toxin with preservation of extracellular vesicles properties, uptake and cargo activity bioRxiv 2023-11-15 (Western Blot, Human)
• Shirley N Tang, Ana I Salazar-Puerta, Mary K Heimann, Kyle Kuchynsky, María A Rincon-Benavides, Mia Kordowski, Gilian Gunsch, Lucy Bodine, Khady Diop, Connor Gantt, Safdar Khan, Anna Bratasz, Olga Kokiko-Cochran, Julie Fitzgerald, Damien M Laudier, Judith A Hoyland, Benjamin A Walter, Natalia Higuita-Castro, Devina Purmessur Engineered extracellular vesicle-based gene therapy for the treatment of discogenic back pain. Biomaterials 2024-04-01 [PMID: 38583365]
• Sarah E. Cummings, Sean P. Delaney, Frederic St-Denis Bissonnette, Andrew Stalker, Gauri Muradia, Jelica Mehic, Tyson E. Graber, Tommy Alain, Jessie R. Lavoie SARS-CoV-2 antigen-carrying extracellular vesicles activate T cell responses in a human immunogenicity model iScience 2023-12-12 [PMID: 38226155]
• Pan Y, Chen T, Zhang Q et al. Highly Selective Purification of Plasma Extracellular Vesicles Using Titanium Dioxide Microparticles for Depicting the Metabolic Signatures of Diabetic Retinopathy Analytical chemistry 2022-10-18 [PMID: 36197877]
• de la Cruz-Ojeda P, Schmid T, Boix L et al. miR-200c-3p, miR-222-5p, and miR-512-3p Constitute a Biomarker Signature of Sorafenib Effectiveness in Advanced Hepatocellular Carcinoma Cells 2022-08-28 [PMID: 36078082] (WB, Human)
• Kiyga E, Adıguzel Z, Onay Ucar E Temozolomide increases heat shock proteins in extracellular vesicles released from glioblastoma cells Molecular biology reports 2022-06-25 [PMID: 35752701] (WB, Human)
• Sung M, Sung S, Kang K Et al. Serum-Derived Neuronal Exosomal microRNAs as Stress-Related Biomarkers in an Atopic Dermatitis Model Biomedicines 2021-11-25 [PMID: 34944580] (WB, Mouse)
• Yin GN, Shin TY, Ock J et al. Pericyte derived extracellular vesicles mimetic nanovesicles improves peripheral nerve regeneration in mouse models of sciatic nerve transection International journal of molecular medicine 2022-02-01 [PMID: 34935051] (WB, Mouse)
• Sung M, Sung SE, Kang KK et al. Serum-Derived Neuronal Exosomal miRNAs as Biomarkers of Acute Severe Stress International journal of molecular sciences 2021-09-15 [PMID: 34576126] (WB, Rat)
• Sung SE, Seo MS, Kang KK et al. Isolation and Characterization of Extracellular Vesicle from Mesenchymal Stem Cells of the Epidural Fat of the Spine Asian Spine Journal 2022-04-30 [PMID: 34461688] (Western Blot, Human)
• Giacomini E, Scotti Gm, Vanni Vs Et Al. Global transcriptomic changes occur in uterine fluid-derived extracellular vesicles during the endometrial window for embryo implantation Human reproduction (Oxford, England) 2021-07-19 [PMID: 34190319] (WB)
• Hu S, Li Z, Shen D et al. Exosome-eluting stents for vascular healing after ischaemic injury Nature biomedical engineering 2021-04-05 [PMID: 33820981]
• Abu N, Othman N, Ab Razak NS et al. Extracellular Vesicles Derived From Colorectal Cancer Affects CD8 T Cells: An Analysis Based on Body Mass Index Frontiers in Cell and Developmental Biology 2020-11-26 [PMID: 33324632] (Western Blot, Human)
• Deng Z, Wang Y, Hu L et al. A facile, rapid, high-throughput extracellular vesicles analytical platform for cancer detection Analytica Chimica Acta 2020-11-01 [PMID: 33161974] (WB, Human)
• Zhou X, Brown BA, Siegel AP et al. Exosome-Mediated Crosstalk between Keratinocytes and Macrophages in Cutaneous Wound Healing ACS Nano 2020-09-15 [PMID: 32931251] (FLOW)
• Gori A, Romanato A et al. Membrane-binding peptides for extracellular vesicles on-chip analysis. J Extracell Vesicles 2020-05-05 [PMID: 32363015] (WB, Human)
• Ye W, Pan R, Shi K et al. Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS Biosensors and Bioelectronics: X 2022-05-01 (WB, Human)
• Zhang L, Li R, Geng R et al. Tumor Susceptibility Gene 101 (TSG101) Contributes to Virion Formation of Porcine Reproductive and Respiratory Syndrome Virus via Interaction with the Nucleocapsid (N) Protein along with the Early Secretory Pathway Journal of virology 2022-01-26 [PMID: 35080428] (ICC/IF, Monkey)
• Murdica V, Giacomini E et al. In vitro cultured human endometrial cells release extracellular vesicles that can be uptaken by spermatozoa. Sci Rep 2020-01-06 [PMID: 32483153] (WB, Human)
• Hough KP, Deshane JS. Cutting edge approaches for rapid characterization of airway exosomes Methods 2020-01-19 [PMID: 31953152] (Func)
  
  Details:
  Citation using the FITC version of this antibody.
• Kwon MH, Song KM, Limanjaya A, et al. Embryonic stem cell-derived extracellular vesicle-mimetic nanovesicles rescue erectile function by enhancing penile neurovascular regeneration in the streptozotocin-induced diabetic mouse Sci Rep 2019-12-27 [PMID: 31882614] (WB, Mouse)
• Uygur B, Melikov K, Arakelyan A et al. Syncytin 1 dependent horizontal transfer of marker genes from retrovirally transduced cells Sci Rep 2019-11-27 [PMID: 31776415] (WB, Mouse, Human)
• Hon KW, Ab-Mutalib NS, Abdullah NMA et al. Extracellular Vesicle-derived circular RNAs confers chemoresistance in Colorectal cancer Sci Rep 2019-11-11 [PMID: 31712601] (WB, Human)
• Murdica, V, Cermisoni, G C Et al. Proteomic analysis reveals the negative modulator of sperm function glycodelin as over-represented in semen exosomes isolated from asthenozoospermic patients. Hum Reprod 2019-08-01 [PMID: 31355853] (WB, Mouse)
• Murdica, V;Giacomini, E;Alteri, A;Bartolacci, A;Cermisoni, GC;Zarovni, N;Papaleo, E;Montorsi, F;Salonia, A;Vigano, P;Vago, R; Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation Fertil. Steril. 2019-05-01 [PMID: 31029245] (WB, Human)
• Christodoulou K, Minaidou A, Nicolaou P. Deregulation of LRSAM1 expression impairs the levels of TSG101, UBE2N, VPS28, MDM2 and EGFR PLoS One. [PMID: 30726272] (WB, Human)
• Hough kP, Trevor JL, Strenkowski JG et al. Exosomal transfer of mitochondria from airway myeloid-derived regulatory cells to T cells. Redox Biol. 2018-06-25 [PMID: 29986209] (FLOW, Human)
• Hough kP, Wilson LS, Trevor JL et al. Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics. Sci Rep. 2018-07-09 [PMID: 29985427] (FLOW, Human)
• Guix FX, Corbett GT, Cha DJ et al. Detection of Aggregation-Competent Tau in Neuron-Derived Extracellular Vesicles. Int J Mol Sci. 2018-02-27 [PMID: 29495441] (ELISA, Human)
• Boopathy GTK, Kulkarni M, Ho SY et al. Cavin-2 regulates the activity and stability of endothelial nitric oxide synthase (eNOS) in angiogenesis J. Biol. Chem. 2017-09-14 [PMID: 28912276] (Mouse)
• Gschweitl M, Ulbricht A, Barnes CA et al. A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes. Elife. 2016-04-22 [PMID: 27008177] (WB, Human)
• Sagar G, Sah R P et al. Pathogenesis of pancreatic cancer exosome-induced lipolysis in adipose tissue. Gut 2016-01-07 [PMID: 26061593] (WB, Mouse)
• Shabbir A, Coz A, Rodriguez L et al. Mesenchymal stem cell exosomes induce the proliferation and migration of normal and chronic wound fibroblasts, and enhance angiogenesis in vitro Stem Cells Dev. 2015-04-13 [PMID: 25867197]
• Choy RW, Cheng Z, Schekman R et al. Amyloid precursor protein (APP) traffics from the cell surface via endosomes for amyloid B (AB) production in the trans-Golgi network. Proc Natl Acad Sci USA 2012-07-01 [PMID: 22711829]
• Vanni VS UTERINE FLUID EXTRACELLULAR VESICLES AS A LIQUID BIOPSY FOR THE DIAGNOSIS OF ENDOMETRIAL RECEPTIVITY Thesis 2022-01-01 (WB, Human)
• Toyofuku T, Nojima S, Ishikawa T et al. Endosomal sorting by Semaphorin 4A in retinal pigment epithelium supports photoreceptor survival. Genes Dev 2012-03-30 [PMID: 22465952] (WB, Human)
• Nabhan JF, Hu R, Oh RS et al. Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein. Proc Natl Acad Sci USA 2012-03-01 [PMID: 22315426] (WB, Human)
• Rush JS, Quinalty LM, Engelman L et al. Endosomal accumulation of the activated epidermal growth factor receptor (EGFR) induces apoptosis. J Biol Chem 2012-01-01 [PMID: 22102283]
• Tampellini D, Rahman N, Lin MT et al. Impaired B-amyloid secretion in Alzheimer's disease pathogenesis. J Neurosci 2011-10-01 [PMID: 22031884] (ICC/IF, Mouse)
• Falguieres T, Castle D, Gruenberg J. Regulation of the MVB pathway by SCAMP3. Traffic 2012-01-01 [PMID: 21951651]
• Pasqual G, Rojek JM, Masin M et al. Old world arenaviruses enter the host cell via the multivesicular body and depend on the endosomal sorting complex required for transport. PLoS Pathog 2011-09-01 [PMID: 21931550] (WB, Human)
• Eyster CA, Cole NB, Petersen S et al. MARCH ubiquitin ligases alter the itinerary of clathrin-independent cargo from recycling to degradation. Mol Biol Cell 2011-09-01 [PMID: 21757542] (WB, Human)
• Hahn S, Setz C, Wild J et al. The PTAP sequence within the p6 domain of human immunodeficiency virus type 1 Gag regulates its ubiquitination and MHC class I antigen presentation. J Immunol 2011-05-01 [PMID: 21482733] (WB, Human)
• Battke C, Ruiss R, Welsch U et al. Tumour exosomes inhibit binding of tumour-reactive antibodies to tumour cells and reduce ADCC. Cancer Immunol Immunother 2011-05-01 [PMID: 21293856] (Human)
• Dynek JN, Goncharov T, Dueber EC et al. c-IAP1 and UbcH5 promote k11-linked polyubiquitination of RIP1 in TNF signalling. EMBO J 2010-12-01 [PMID: 21113135] (WB, Human)
• Hayer A, Stoeber M, Ritz D et al. Caveolin-1 is ubiquitinated and targeted to intralumenal vesicles in endolysosomes for degradation. J Cell Biol 2010-11-01 [PMID: 21041450] (Human)
• Apaja PM, Xu H, Lukacs GL et al. Quality control for unfolded proteins at the plasma membrane. J Cell Biol 2010-11-01 [PMID: 20974815] (WB, Human)
• Balut CM, Gao Y, Murray SA et al. ESCRT-dependent targeting of plasma membrane localized kCa3.1 to the lysosomes. Am J Physiol Cell Physiol 2010-11-01 [PMID: 20720181] (ICC/IF, Human)
• Inuzuka T, Suzuki H, Kawasaki M et al. Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2DeltaGF122) and structural roles of F122 in target recognition. BMC Struct Biol 2010-08-01 [PMID: 20691033] (WB, Human)
• Moorman NJ, Sharon-Friling R, Shenk T et al. A targeted spatial-temporal proteomics approach implicates multiple cellular trafficking pathways in human cytomegalovirus virion maturation. Mol Cell Proteomics 2010-05-01 [PMID: 20023299] (ICC/IF, Human)
• Leithe E, Kjenseth A, Sirnes S et al. Ubiquitylation of the gap junction protein connexin-43 signals its trafficking from early endosomes to lysosomes in a process mediated by Hrs and Tsg101. J Cell Sci 2009-11-01 [PMID: 19808888] (ICC/IF, Rat)
• Chen J, Wang J, Meyers KR et al. Transferrin-directed internalization and cycling of transferrin receptor 2. Traffic 2009-10-01 [PMID: 19682329] (WB, Human)
• Truschel ST, Simoes S, Setty SR et al. ESCRT-I function is required for Tyrp1 transport from early endosomes to the melanosome limiting membrane. Traffic 2009-09-01 [PMID: 19624486] (WB, Human)
• Okumura M, Ichioka F, Kobayashi R et al. Penta-EF-hand protein ALG-2 functions as a Ca2+-dependent adaptor that bridges Alix and TSG101. Biochem Biophys Res Commun 2009-08-01 [PMID: 19520058] (WB)
• Glozman R, Okiyoneda T, Mulvihill CM et al. N-glycans are direct determinants of CFTR folding and stability in secretory and endocytic membrane traffic. J Cell Biol 2009-03-01 [PMID: 19307599] (WB, Human)
• Aoh QL, Castle AM, Hubbard CH et al. SCAMP3 negatively regulates epidermal growth factor receptor degradation and promotes receptor recycling. Mol Biol Cell 2009-03-01 [PMID: 19158374] (WB, Human)
• Luyet PP, Falguieres T, Pons V et al. The ESCRT-I subunit TSG101 controls endosome-to-cytosol release of viral RNA. Traffic 2008-12-01 [PMID: 18817529] (WB, Human)
• Falguieres T, Luyet PP, Bissig C et al. In vitro budding of intralumenal vesicles into late endosomes is regulated by Alix and Tsg101. Mol Biol Cell 2008-11-01 [PMID: 18768755]
• Valiathan RR, Resh MD. Differential control of CXCR4 and CD4 downregulation by HIV-1 Gag. Virol J 2008-02-01 [PMID: 18267010] (WB, Primate)
• Shibata H, Suzuki H, Kakiuchi T et al. Identification of Alix-type and Non-Alix-type ALG-2-binding sites in human phospholipid scramblase 3: differential binding to an alternatively spliced isoform and amino acid-substituted mutants. J Biol Chem 2008-04-01 [PMID: 18256029] (WB, Human)
• Ichioka F, Kobayashi R, Katoh K et al. Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4). FEBS J 2008-02-01 [PMID: 18190528] (WB, Human)
• Filimonenko M, Stuffers S, Raiborg C et al. Functional multivesicular bodies are required for autophagic clearance of protein aggregates associated with neurodegenerative disease. J Cell Biol 2007-11-01 [PMID: 17984323] (WB, Human)
• Fraile-Ramos A, Pelchen-Matthews A, Risco C et al. The ESCRT machinery is not required for human cytomegalovirus envelopment. Cell Microbiol 2007-12-01 [PMID: 17760879] (WB)
• Young, TW et al. Up-regulation of Tumor Susceptibility Gene 101 Conveys Poor Prognosis through Suppression of p21 Expression in Ovarian Cancer. Clin Cancer Res 13: 3848-3854. 2007-01-01 [PMID: 17606716]
• De Domenico I, Ward DM, Langelier C et al. The molecular mechanism of hepcidin-mediated ferroportin down-regulation. Mol Biol Cell 2007-07-01 [PMID: 17475779] (WB, Human)
• Tampellini D, Magrane J, Takahashi RH et al. Internalized antibodies to the Abeta domain of APP reduce neuronal Abeta and protect against synaptic alterations. J Biol Chem 2007-06-01 [PMID: 17468102] (ICC/IF, Mouse)
• Young, T W et al. Up-regulation of Tumor Susceptibility Gene 101 Protein in Ovarian Carcinomas Revealed by Proteomics Analyses. Mol Cell Proteomics 6: 294-304. 2007-01-01 [PMID: 17110434]
• Langelier C, von Schwedler UK, Fisher RD et al. Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release. J Virol 2006-10-01 [PMID: 16973552] (WB, ICC/IF, Human)
• Chertova E, Chertov O, Coren LV et al. Proteomic and biochemical analysis of purified human immunodeficiency virus type 1 produced from infected monocyte-derived macrophages. J Virol 2006-09-01 [PMID: 16940516] (WB, Human)
• Raiborg C, Wesche J, Malerod L et al. Flat clathrin coats on endosomes mediate degradative protein sorting by scaffolding Hrs in dynamic microdomains. J Cell Sci 2006-06-01 [PMID: 16720641] (ICC/IF, Human)
• Razi M, Futter CE. Distinct roles for Tsg101 and Hrs in multivesicular body formation and inward vesiculation. Mol Biol Cell 2006-08-01 [PMID: 16707569] (WB, Human)
• Almeida CG, Takahashi RH, Gouras GK. Beta-amyloid accumulation impairs multivesicular body sorting by inhibiting the ubiquitin-proteasome system. J Neurosci 2006-04-01 [PMID: 16624948] (ICC/IF, Mouse)
• Duncan LM, Piper S, Dodd RB et al. Lysine-63-linked ubiquitination is required for endolysosomal degradation of class I molecules. EMBO J 2006-04-01 [PMID: 16601694]
• Bache KG, Stuffers S, Malerod L et al. The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor. Mol Biol Cell 2006-06-01 [PMID: 16554368]
• Hui EK, Barman S, Tang DH et al. YRkL sequence of influenza virus M1 functions as the L domain motif and interacts with VPS28 and Cdc42. J Virol 2006-03-01 [PMID: 16474136] (WB, Human)
• Gullapalli A, Wolfe BL, Griffin CT et al. An essential role for SNX1 in lysosomal sorting of protease-activated receptor-1: evidence for retromer-, Hrs-, and Tsg101-independent functions of sorting nexins. Mol Biol Cell 2006-03-01 [PMID: 16407403] (WB, Human)
• Irie T, Harty RN. L-domain flanking sequences are important for host interactions and efficient budding of vesicular stomatitis virus recombinants. J Virol 2005-10-01 [PMID: 16188963] (IP, Human)
• Cabezas A, Bache KG, Brech A et al. Alix regulates cortical actin and the spatial distribution of endosomes. J Cell Sci 2005-06-01 [PMID: 15914539] (WB, Human)
• Irie T, Licata JM, Harty RN et al. Functional characterization of Ebola virus L-domains using VSV recombinants. Virology 2005-06-01 [PMID: 15892969] (IP, Human)
• Ward DM, Vaughn MB, Shiflett SL et al. The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells. J Biol Chem 2005-03-01 [PMID: 15644320] (WB, Human)
• Lin Y, Kimpler LA, Naismith TV et al. Interaction of the mammalian endosomal sorting complex required for transport (ESCRT) III protein hSnf7-1 with itself, membranes, and the AAA+ ATPase SkD1. J Biol Chem 2005-04-01 [PMID: 15632132] (WB, Human)
• Bache KG, Slagsvold T, Cabezas A et al. The growth-regulatory protein HCRP1/hVps37A is a subunit of mammalian ESCRT-I and mediates receptor down-regulation. Mol Biol Cell 2004-09-01 [PMID: 15240819] (WB, Human)
• Stuchell MD, Garrus JE, Muller B et al. The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding. J Biol Chem 2004-08-01 [PMID: 15218037] (WB, Human)
• Sharma M, Pampinella F, Nemes C et al. Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes. J Cell Biol 2004-03-01 [PMID: 15007060] (WB)
• Zhu G, Gilchrist R, Borley N et al. Reduction of TSG101 protein has a negative impact on tumor cell growth. Int J Cancer 2004-04-01 [PMID: 14991575] (WB, Human)
• Irie T, Licata JM, McGettigan JP et al. Budding of PPxY-containing rhabdoviruses is not dependent on host proteins TGS101 and VPS4A. J Virol 2004-03-01 [PMID: 14990685] (IP, Human)
• Heissmeyer V, Macian F, Im SH et al. Calcineurin imposes T cell unresponsiveness through targeted proteolysis of signaling proteins. Nat Immunol 2004-03-01 [PMID: 14973438] (WB, Mouse)
• von Schwedler UK, Stuchell M, Muller B et al. The protein network of HIV budding. Cell 2003-09-01 [PMID: 14505570] (WB)
• Strack B, Calistri A, Craig S et al. AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding. Cell 2003-09-01 [PMID: 14505569] (WB, Human)
• Chiou CT, Hu CC, Chen PH et al. Association of Japanese encephalitis virus NS3 protein with microtubules and tumour susceptibility gene 101 (TSG101) protein. J Gen Virol 2003-10-01 [PMID: 13679614] (ICC/IF, Hamster)
• Gottwein E, Bodem J, Muller B et al. The Mason-Pfizer monkey virus PPPY and PSAP motifs both contribute to virus release. J Virol 2003-09-01 [PMID: 12915562] (WB, Human)
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• Pornillos O, Higginson DS, Stray KM et al. HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein. J Cell Biol 2003-08-01 [PMID: 12900394] (WB, Human)
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• Myers EL, Allen JF. Tsg101, an inactive homologue of ubiquitin ligase e2, interacts specifically with human immunodeficiency virus type 2 gag polyprotein and results in increased levels of ubiquitinated gag. J Virol 2002-11-01 [PMID: 12388682] (WB, Human)
• Bishop N, Horman A, Woodman P et al. Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates. J Cell Biol 2002-04-01 [PMID: 11916981] (WB, Human, Mouse)
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Reviews for TSG101 Antibody (NB200-112) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer WB Human 10/20/2022

Summary

• Application: Western Blot
• Sample Tested: MDA-MB-231
• Species: Human
• Lot: 44501

reviewed by: Elizabeth Soberg WB Mouse 06/05/2018

Summary

• Application: Western Blot
• Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line
• Species: Mouse
• Lot: 41449

Comments

• Comments: Used primary @ 1:500 and Donkey Anti-Mouse @ 0.5 ug/mL (this could be titrated down to reduce background). Used picogram sensitivity ECL reagents.

reviewed by: Verified Customer WB Mouse 12/08/2017

Summary

• Application: Western Blot
• Sample Tested: Primary mouse hepatocytes
• Species: Mouse
• Lot: 41449

Comments

• Comments: mouse hepatocytes were treated with 100 ng/ml LPS for 4 hours

Bioinformatics

• Gene Symbol: TSG101
• Entrez:
  • 7251(Human)
  • 22088(Mouse)
• Uniprot:
  • Q99816(Human)