Recombinant Human TWEAK R/TNFRSF12 Protein

SDS-Page: Recombinant Human TWEAK R/TNFRSF12 Protein [NBP2-35085]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human TWEAK R/TNFRSF12 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 53 amino acids corresponding to TWEAK R/TNFRSF12 Source: E. coli

  Uniprot ID: Q9NP84

  Amino Acid Sequence: EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP

• Details of Functionality: TWEAK R/TNFRSF12 protein is fully biologically active when compared to standard. The ED50 as determined by inhibiting TWEAK-dependent proliferation of human umbilical vein endothelial cells (HUVEC) is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x104 IU/mg, in the presence of 15 ng/ml of rHuTWEAK
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TNFRSF12A
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of TWEAK R/TNFRSF12 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 5.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human TWEAK R/TNFRSF12 Protein

• CD266 antigen
• CD266
• FGF-inducible 14
• Fibroblast growth factor-inducible immediate-early response protein 14
• Fn14
• FN14tumor necrosis factor receptor superfamily member 12A
• TNFRSF12
• TNFRSF12A
• tumor necrosis factor receptor superfamily, member 12A
• TWEAK R
• TWEAK Receptor/TNFRSF12A
• TWEAKR
• tweak-receptor
• type I transmembrane protein Fn14

Background

Human TNF-related weak inducer of apoptosis receptor (TWEAKR) also known as Tumor necrosis factor receptor superfamily member 12A precursor (gene name TNFRSF12A) or fibroblast growth factor-inducable 14 kDa protein, is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. It is expressed in the spleen, thymus, peripheral blood lymphocytes, colon, and small intestine. Signal transduction by TWEAK receptor can be activated by either the membrane anchored or the soluble TWEAK. In addition, It plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. Human and mouse TWEAK R share 82 % a.a. sequence identity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF12A
• Uniprot:
  • Q9NP84()
  • Q9NP84()
  • Q9NP84()