Twist-2 Antibody

Western Blot: Twist-2 Antibody [H00117581-D01P] - Analysis of TWIST2 expression in transfected 293T cell line by TWIST2 polyclonal antibody.Lane 1: TWIST2 transfected lysate(18.10 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Twist-2 Antibody [H00117581-D01P] - Analysis of antibody to TWIST2 on HeLa cell. Antibody concentration 10 ug/ml.

Product Discontinued

Twist-2 Antibody Summary

• Immunogen: TWIST2 (NP_476527.1, 1 a.a. - 160 a.a.) full-length human protein. MEEGSSSPVSPVDSLGTSEEELERQPKRFGRKRRYSKKSSEDGSPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTSCSYVAHERLSYAFSVWRMEGAWSMSASH
• Specificity: Reacts with twist homolog 2 (Drosophila).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TWIST2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This antibody is reactive against transfected lysate in western blot.

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 22884497)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Twist-2 Antibody

• bHLHa39
• bHLHa39twist-related bHLH protein Dermo1
• Class A basic helix-loop-helix protein 39
• Dermis-expressed protein 1
• DERMO1
• Dermo-1
• DERMO1MGC117334
• twist homolog 2 (Drosophila)
• Twist2
• Twist-2
• twist-related protein 2

Background

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Twist. It is thought that during osteoblast development this protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Twist-2 Antibody (H00117581-D01P)(1)

Publication using H00117581-D01P

• Wade C, Brinas I, Welfare M et al. Twist2 contributes to termination of limb bud outgrowth and patterning through direct regulation of Grem1. Dev Biol. 2012-10-01 [PMID: 22884497] (Chicken)

Bioinformatics

• Gene Symbol: TWIST2
• Entrez:
  • 117581(Human)
• Uniprot:
  • NP_476527.1(Human)