Tyk2 Antibody

Western Blot: Tyk2 Antibody [NBP2-76968] - Western blot analysis of TYK2 on MCF-7 cell lysate using anti-TYK2 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Tyk2 Antibody [NBP2-76968] - ICC staining TYK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Tyk2 Antibody [NBP2-76968] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti- TYK2 antibody. Counter stained with hematoxylin.

Flow Cytometry: Tyk2 Antibody [NBP2-76968] - Flow cytometric analysis of MCF-7 cells with TYK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: Tyk2 Antibody [NBP2-76968] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TYK2 antibody. Counter stained with hematoxylin.

Immunohistochemistry: Tyk2 Antibody [NBP2-76968] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- TYK2 antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Tyk2 Antibody Summary

• Immunogen: Recombinant protein within human Tyk2 aa 390-620. (SwissProt: P29597 Human; SwissProt: Q9R117 Mouse)
• Localization: Cytoskeleton. Nucleus. Cytosol. Plasma membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TYK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 134 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Tyk2 Antibody

• EC 2.7.10
• EC 2.7.10.2
• JTK1
• non-receptor tyrosine-protein kinase TYK2
• Tyk2
• tyrosine kinase 2

Background

Novel non-receptor tyrosine-protein kinase, Tyk2, belongs to the tyrosine protein kinase family and plays a role in intracellular signal transduction by being involved in the initiation of type I IFN signaling. Tyk2 is believed to phosphorylate the interferon-alpha/beta receptor alpha chain (1). It has been shown that Tyk2 links the interferon alpha/beta receptor to the cytoplasmic transcription factor that mediates activation of interferon-responsive genes (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TYK2