Recombinant Human UBE2J2/UBC6 Protein

Product Discontinued

Recombinant Human UBE2J2/UBC6 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-226 of Human UBE2J2/UBC6
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSSTSS KRAPTTATQR LKQDYLRIKK DPVPYICAEP LPSNILEWHY VVRGPEMTPY EGGYYHGKLI FPREFPFKPP SIYMITPNGR FKCNTRLCLS ITDFHPDTWN PAWSVSTILT GLLSFMVEKG PTLGSIETSD FTKRQLAVQS LAFNLKDKVF CELFPEVVEE IKQKQKAQDE LSSRPQTLPL PDVVPDGETH LVQNGIQLLN GHAPGAVPNL AGLQQANRHH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2J2
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 30% glycerol, 1 mM DTT, 200 mM NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human UBE2J2/UBC6 Protein

• EC 6.3.2.19
• J2
• NCUBE2
• PRO2121
• Ubc6
• Ubc6p
• UBE2J2
• ubiquitin-conjugating enzyme E2 J2
• ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast)
• yeast UBC6 homolog

Background

UBE2J2, also known as ubiquitin-conjugating enzyme E2 J2, belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Recombinant human UBE2J2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2J2