Ulex Europaeus Lectin 1 Antibody

Immunohistochemistry: Ulex Europaeus Lectin 1 Antibody [NB110-13922] - Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:1000 Anti-Lectin, Ulex europaeus-I antibody produced in Rabbit followed by 1:30 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat.

• Reactivity: Pl
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Ulex Europaeus Lectin 1 Antibody Summary

• Immunogen: Anti-Ulex Europaeus-I (UEA-I) Lectin is developed in rabbit using purified Ulex Europaeus-I lectin from gorse seeds as the immunogen.
• Marker: Endothelial Cells
• Specificity: The antibody may be used for studies of the vascular structures. It reacts specifically with UEA-I bound to human endothelial cells of normal and neoplastic blood and lymphatic vessels. Its uniform reaction with vessels enables differentation between vessels and artificial slits in the tissue.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:500
• Application Notes: Lectin isolated from Ulex europaeus I (NBP2-42700) is specific for alpha-L-fucose-containing glycocompounds which (in combination with NB110-13922) makes it a specific marker for vascular endothelial cells and tumors derived from them. For immunoassays on animal tissues, Ulex Europaeus Lectin 1 Protein (NBP2-42700) would be a required product alongwith NB110-13922. Technically, NBP2-42700 will bind the carbohydrate moiety on vascular endothelial cells as first primary reagent and then NB110-13922 will bind to Ulex Europaeus-1 lectin (NBP2-42700) in the primary antibody incubation step (See product specific IHC-P Protocol).
• Control:

  Ulex Europaeus Lectin 1 Native Protein

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera, delipidized
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Ulex Europaeus Lectin 1 Antibody

• Gorse or Furze Lectin
• UEA-1; H(O) lectin 1; Anti H(O) lectin I

Background

Lectins are highly specific carbohydrate binding proteins useful in carbohydrate and cell membrane studies, as well as cell agglutination and typing techniques. Anti-Ulex Europaeus-I (UEA-I) Lectin is developed in rabbit using purified Ulex Europaeus-I lectin from gorse seeds as the immunogen. Ulex Europaeus Lectin I is a 46 kDa glycoprotein known to interact with alpha-I fucosyl residues in oligosaccharides present on the membranes of human blood group O erythrocytes, human endothelial cells and a variety of human and animal epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ulex Europaeus Lectin 1 Antibody (NB110-13922)(2)

Publications using NB110-13922

• GHAZALI M, AB-RAHIM S, MUHAMAD M EXPRESSION OF THE MICROFOLD CELLS IN THREE-DIMENSIONAL COCULTURE SYSTEM FOR IN VITRO CULTIVATION OF HUMAN NOROVIRUS Int J App Pharm 2019-09-21
• Ding L, Chakrabarti J, Sheriff S et al. Toll Like Receptor 9 Pathway Mediates Schlafen+-MDSC Polarization During Helicobacter-Induced Gastric Metaplasias Gastroenterology 2022-04-26 [PMID: 35487288] (IF/IHC, Mouse)