Vinculin Antibody (VIN-11-5)

Western Blot: Vinculin Antibody (VIN-11-5) [NB120-11193] - Chicken gizzard cell extract was separated on SDS-PAGE and probed with NB120-11193. The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBT/BCIP substrate. Antibody dilution 1/200.

Immunocytochemistry/Immunofluorescence: Vinculin Antibody (VIN-11-5) [NB120-11193] - Double labeling of an isolated smooth muscle cell (chicken gizzard) with Monoclonal Anti-Vinculin, clone no. VIN-11-5 (green) and Anti-alpha Actinin (red).

• Reactivity: Hu, Mu, Bv, Ch
• Applications: WB, ICC/IF, MA
• Clone: VIN-11-5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Vinculin Antibody (VIN-11-5) Summary

• Immunogen: Smooth muscle vinculin from chicken gizzard.
• Localization: Cytoplasmic
• Marker: Focal Adhesion Marker
• Specificity: The reacts best with cultured chicken fibroblasts. Labeling also may be obtained with bovine, human, or mouse cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCL
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Microarray
  • Western Blot 1:100-1:2000
• Theoretical MW: 116 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Vinculin Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Vinculin Antibody (VIN-11-5)

• CMD1W
• CMH15
• metavinculin
• MVCL
• VCL
• Vinculin

Background

Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell extracellular matrix adherens type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. The sequences of chicken, nematode and human vinculin have been determined. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha actinin and possibly with itself. Since alpha actinin binds to and cross links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vinculin Antibody (NB120-11193)(1)

Publication using NB120-11193

• Oshima, K, Zhao, J Et al. Mutational and functional genetics mapping of chemotherapy resistance mechanisms in relapsed acute lymphoblastic leukemia. Nat Cancer 2020-11-01 [PMID: 33796864]

FAQs for Vinculin Antibody (NB120-11193). (Showing 1 - 1 of 1 FAQ).

1. Do you have any 100 coda or bigger controls?
   • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Bioinformatics

• Gene Symbol: VCL
• Entrez:
  • 396422(Chicken)
  • 7414(Human)
  • 22330(Mouse)
• Uniprot:
  • P12003(Chicken)