Wnt-4 Antibody - BSA Free

Western Blot: Wnt-4 Antibody [NBP2-20909] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT4 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Wnt-4 Antibody [NBP2-20909] - Mouse kidney. WNT4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Wnt-4 Antibody [NBP2-20909] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: WNT4 transfected 293T lysates 10% SDS PAGE diluted at 1:5000

Western Blot: Wnt-4 Antibody [NBP2-20909] - Non-transfected (-) and Wnt4-transfected (+, including V5-tag) 293T whole cell extracts (100 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT4 antibody at a dilution of 1:2500. The V5 was used as internal control, (1:2500) shown at the bottom panel.

Immunohistochemistry-Paraffin: Wnt-4 Antibody [NBP2-20909] - U87 xenograft, using WNT4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Wnt-4 Antibody [NBP2-20909] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Wnt-4 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Wnt-4. The exact sequence is proprietary.
• Localization: Secreted; extracellular space; extracellular matrix
• Predicted Species: Xenopus (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WNT4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Japanese medaka (85%), Chicken (89%), Xenopus laevis (90%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Wnt-4 Antibody - BSA Free

• protein Wnt-4
• SERKAL
• wingless-type MMTV integration site family, member 4
• Wnt4
• Wnt-4

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Wnt-4 Antibody (NBP2-20909)(1)

Publication using NBP2-20909

• Lin CW Jan MS Kuo JH. Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine. Mol Pharm. [PMID: 26158199]

Bioinformatics

• Gene Symbol: WNT4
• Uniprot:
  • P56705(Human)