Zinc finger protein 639 Antibody

Western Blot: Zinc finger protein 639 Antibody [NBP2-15177] - Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Zinc finger protein 639 Antibody [NBP2-15177] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZNF639 protein stained by ZNF639 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: Zinc finger protein 639 Antibody [NBP2-15177] - Paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.

Product Discontinued

Zinc finger protein 639 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ZNF639. The exact sequence is proprietary.
• Predicted Species: Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF639
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (80%), Chicken (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Zinc finger protein 639 Antibody

• ANC_2H01
• ZASC1ANC-2H01
• zinc finger amplified in esophageal squamous cell carcinomas 1
• zinc finger protein 639
• Zinc finger protein ANC_2H01
• Zinc finger protein ZASC1,6230400O18Rik

Background

Zinc finger protein 639 binds DNA and may function as a transcriptional repressor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for Zinc finger protein 639 Antibody (NBP2-15177). (Showing 1 - 1 of 1 FAQ).

1. We would like to know about the staining pattern of NBP2-15177. I checked Uniprot and found ZNF639 shall be expressed at nucleus, but later I found it also presents at cytoplasm based on the info from http://www.proteinatlas.org/ENSG00000121864/tissue.
   • It is a relatively new and less published target, and it will be too early to make any generalization on the cellular localization of ZNF639 protein. Imoto et al. 2003 (Identification of ZASC1 encoding a Kruppel-like zinc finger protein as a novel target for 3q26 amplification in esophageal squamous cell carcinomas.) performed their studies on transfected cells and found the overexpressed protein to be nuclear, however, on page 5693, column 2, last sentence of paragraph 1 of their research paper, they wrote that : " although the subcellular localization should be confirmed by investigating the presence of endogenous ZASC1 using specific antibody, our result suggested that ZASC1 might function as a nuclear transcription factor". Bogaerts et al.2005 (Nuclear translocation of alphaN-catenin by the novel zinc finger transcriptional repressor ZASC1.) tested the subcellular localization of the endogenous ZASC1 protein in MCF7/AZ cells with monoclonal antibody 8D4/1 and reported it as a nuclear target. However, from the images shown in this article, it looks like besides strong nuclear expression, this target shows mild cytoplasmic expression also (endogenous as well as the overexpression situations)! Human Protein Atlas team tested ZASC1 in 81 normal tissue/cell types, and they found a positive signal in 45 of the tested sample types! They employed two different antibodies (specificity tested with WB and protein array), and found nuclear as well as cytoplasmic staining. In our testing with #NBP2-15177 in HBL435 xenograft, we observed strong nuclear with mild cytoplasmic staining.

Bioinformatics

• Gene Symbol: ZNF639
• Uniprot:
  • Q9UID6(Human)