HA Tag Antibodies
HA Tag is used extensively as a general epitope tag in expression vectors. The HA tag is frequently added to the N- or C- terminus of a protein of interest to facilitate protein purification, detection, and labeling using anti-HA antibodies. The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa. We offer antibodies to detect HA Tag that are highly reviewed and have been cited in 110+ publications. Browse our extensive catalog of recombinant monoclonal antibodies, monoclonal antibodies, polyclonal antibodies, and HA Tag-conjugated antibodies. Our HA-Tag antibodies are suitable for multiple validated research applications including Chromatin Immunoprecipitation, CyTOF-ready, ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC-Paraffin, IP, Simple Western, and Western Blot. Our HA Tag antibodies can be used in a variety of model species: Human, All Species, Multi-species, Non-species specific, and Virus. Each His Tag antibody is fully covered by Novus’ Guarantee+ to give you peace of mind and support when you need it. Choose the anti-HA Tag antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products.
Alternate Names for HA Tag Antibodies
anti-HA Tag antibody, anti-HA antibody, anti-HA epitope tag antibody, anti-Hemagglutinin antibody
18 Results for "ha-tag" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-363] - Detection of HA-tagged Tubulin in CHO cells transfected with mutant beta-tubulin cDNA encoding an HA epitope tag. Photo contributed by R. Bhattacharya and F. Cabral, University of Texas Medical School at Houston.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-363-img0005.jpg "Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-363] - Detection of HA-tagged Tubulin in CHO cells transfected with mutant beta-tubulin cDNA encoding an HA epitope tag. Photo contributed by R. Bhattacharya and F. Cabral, University of Texas Medical School at Houston.")
![Western Blot: HA Tag Antibody [NB600-363] - 200, 100, or 50 ng of E. coli whole cell lysate expressing amulti-tag fusion protein.Antibody: Affinity purified rabbitanti-HA antibody used for WB at 0.04 ug/ml(1:25,000). Detection: Chemiluminescence with anexposure time of 3 seconds.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NB600-363-img0019.jpg)
![Western Blot: HA Tag Antibody [NB600-363] - Wnt/beta-catenin signaling regulates HySp5 expression. Immunoprecipitation (IP) of HA-tagged HySp5-420 expressed in HEK293T cells together or not with hudeltabeta-Catenin (upper) or huTCF1 (lower). IP was performed with an anti-HA antibody and Co-IP products were detected with the anti-beta-catenin or anti-TCF1 antibodies. Same results were obtained in two independent experiments. Each data point represents one biological independent experiment. Statistical p values: *p <= 0.05, **p <= 0.01, ***p <= 0.001, ****p <= 0.0001 (unpaired t test). Error bars indicate SD. PP primer pair, RLA relative luciferase activity Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-08242-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NB600-363-img0018.jpg)
![Western Blot: HA Tag Antibody [NB600-363] - ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. Selection-independent generation of gene knockout mouse embryonic stem cells using zinc-finger nucleases. PLoS One (2011)](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NB600-363-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-363] - HA-tagged proteins were detected in immersion fixed HEK293 human embryonic kidney cell line transfected with HA-tagged MGAT2 using 1 ug/mL goat anti-HA Tag polyclonal (NB600-362, Novus Biologicals), 1 ug/mL rabbit anti-HA Tag polyclonal (NB600-363, Novus Biologicals). Cells were stained using the appropriate secondary antibody, donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-363-img0013.jpg)
![Western Blot: HA Tag Antibody [NB600-363] - Gene knockout in U2OS.693 cells.(A) Schematic of ZFN-mediated knockout. A ZFN pair (ZFNR & ZFNL) designed to target position 502 in the EGFP gene (E502) creates a DNA double-strand break that is sealed by the error-prone non-homologous end-joining (NHEJ) pathway & hence leads to disruption of the coding sequence. (B) Dose-dependent gene disruption in U2OS.693 cells. U2OS.693 cells that stably express a destabilized EGFP were transfected with increasing amounts of E502-specific ZFN expression vectors (75–1200 ng). The percentage of EGFP-negative cells was determined 6 days post-transfection by flow cytometry (n = 3; indicated is average & standard deviation). E502-WT, ZFN with wild-type FokI domain; E502-OH, ZFN with obligate heterodimeric FokI domain. (C) ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors & pEGFP, cell lysates were probed with antibodies against the HA tag & EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, & 1200 ng. NT, non-transfected cells. (D) Kinetics of EGFP knockout. The graph displays the percentage of EGFP-negative cells (see B) from day 1 to day 6 post-transfection for two vector amounts (n = 3; indicated is average & standard deviation). WT, E502-WT; OH, E502-OH. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22194948), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-363_rabbit-polyclonal-ha-tag-antibody-310202416171456.jpg)
![Immunohistochemistry: HA Tag Antibody [NB600-362] - SUMO1 conjugates are not localized to amyloid plaques. (a) Sagittal brain sections of 24-week-old KI/AD and WT/AD animals were stained using anti-HA antibodies (red), 6E10 antibodies (green) that label amyloid beta 1-42 among other amyloid beta variants (epitope lies within amino acids 3-8 of amyloid beta) and anti-MAP2 antibodies (blue). Sections of the hippocampal subiculum are shown. Images are representatives of three independent experiments. Scale bar, 25 um. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12760) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunohistochemistry-NB600-362-img0011.jpg "Immunohistochemistry: HA Tag Antibody [NB600-362] - SUMO1 conjugates are not localized to amyloid plaques. (a) Sagittal brain sections of 24-week-old KI/AD and WT/AD animals were stained using anti-HA antibodies (red), 6E10 antibodies (green) that label amyloid beta 1-42 among other amyloid beta variants (epitope lies within amino acids 3-8 of amyloid beta) and anti-MAP2 antibodies (blue). Sections of the hippocampal subiculum are shown. Images are representatives of three independent experiments. Scale bar, 25 um. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12760) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: HA Tag Antibody [NB600-362] - Lysates of HEK293T human embryonic kidney cell line transfected with N-terminal HA-tagged MICA, N-terminal HA-tagged LGI-2, mock transfected, C-terminal HA-tagged LRRN5, and C-terminal HA-tagged Trance. PVDF membrane was probed with 1:500 dilution of 1:2500 dilution of goat anti-HA Tag polyclonal (NB600-362, Novus Biologicals), 1:5000 dilution of rabbit anti-HA Tag polyclonal (NB600-363, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG (HAF018).](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NB600-362-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-362] - HA-tagged proteins were detected in immersion fixed HEK293 human embryonic kidney cell line transfected with HA-tagged MGAT2 using 1 ug/mL goat anti-HA Tag polyclonal (NB600-362, Novus Biologicals), 1 ug/mL rabbit anti-HA Tag polyclonal (NB600-363, Novus Biologicals). Cells were stained using the appropriate secondary antibody, donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-362-img0005.jpg)
![Western Blot: HA Tag Antibody - BSA Free [NB600-362] - 200, 67, 22, 7, or 2 ng of E. coli whole cell lysateexpressing a multi-tag fusion protein. Antibodies: Affinitypurified, goat anti-HA antibody used for WBat 0.04 ug/ml (1:25,000). Detection: Chemiluminescencewith an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/HA-Tag-Antibody---BSA-Free-Western-Blot-NB600-362-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-362] - HA tagged RFX6 overexpression in HEK293 stained with HA Tag antibody at a dilution of 1:250. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunocytochemistry-NB600-362-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody (4810) [NBP2-43714] - HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HA stained by HA tag antibody [4810] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-4810-Immunocytochemistry-Immunofluorescence-NBP2-43714-img0006.jpg "Immunocytochemistry/Immunofluorescence: HA Tag Antibody (4810) [NBP2-43714] - HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HA stained by HA tag antibody [4810] diluted at 1:1000. Blue: Hoechst 33342 staining.")
![Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Human HEK293T cells overexpressed with Ly6K-HA. Dution 1:1000. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-4810-Western-Blot-NBP2-43714-img0005.jpg)
![Flow Cytometry: HA Tag Antibody (4810) [NBP2-43714] - HEK293T cells overexpressed with Ly6K-HA stained with HA Epitope Tag Antibody (red) or secondary only (black). Flow cytometry image submitted from a verified customer review.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-4810-Flow-Cytometry-NBP2-43714-img0004.jpg)
![Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Analysis of 293T whole cell lysate/extract A: 20 ug whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 ug of pre-immune mouse IgG C: Immunoprecipitation of HA tagged protein by 2.5 ug of HA tagg antibody 10% SDS-PAGE. The immunoprecipitated HA tagged protein was detected by HA tag antibody [4810] diluted at 1:3000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-4810-Western-Blot-NBP2-43714-img0002.jpg)
![Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Analysis of A: 30 ug HeLa whole cell lysate/extract B: 30 ug whole cell lysate/extract of HA tagged protein expressing HeLa cells 5% SDS-PAGE HA tag antibody [4810] dilution: 1:5000.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-4810-Western-Blot-NBP2-43714-img0003.jpg)
![Western Blot: HA Tag Antibody [NBP2-21581] - Analysis in human HEK293T cells overexpressed with Ly6K-HA. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NBP2-21581-img0017.jpg "Western Blot: HA Tag Antibody [NBP2-21581] - Analysis in human HEK293T cells overexpressed with Ly6K-HA. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NBP2-21581] - Analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-21581-img0003.jpg)
![Flow Cytometry: HA Tag Antibody [NBP2-21581] - HEK293T cells overexpressed with Ly6K-HA stained with HA Epitope Tag Antibody (red) or secondary only (black). Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Flow-Cytometry-NBP2-21581-img0013.jpg)
![ELISA: HA Tag Antibody [NBP2-21581] - An ELISA plate is coated with 50 uL of a DDDDK Tag conjugated BSA at concentration of 5 ug/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK Tag antibody at concentration ranged from 3.1 to 200 ng/mL.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-ELISA-NBP2-21581-img0008.jpg)
![Western Blot: HA Tag Antibody [NBP2-21581] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HA Tag antibody (NBP2-21581) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-21581_rabbit-polyclonal-ha-tag-antibody-910202420134084.jpg)
![Western Blot: HA Tag Antibody [NBP1-70006] - Detection of 1.0 ug of 12-Epitope Tag Protein Marker Lysate containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 30 min at room temperature.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-Western-Blot-NBP1-70006-img0004.jpg "Western Blot: HA Tag Antibody [NBP1-70006] - Detection of 1.0 ug of 12-Epitope Tag Protein Marker Lysate containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 30 min at room temperature.")
![Immunohistochemistry-Paraffin: HA Tag Antibody [NBP1-70006] - Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 um were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol. Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2,000 and reacted at 4C overnight followed by signal detection using horseradish peroxidase with DAB as the chromogenic substrate. Tissue was counterstained with Mayer's hematoxylin. Personal Communication, Behzad Yeganeh,U. Manitoba, Winnipeg, Canada.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-Immunohistochemistry-Paraffin-NBP1-70006-img0001.jpg)
![Western Blot: HA Tag Antibody (RM305) [NBP2-61477] - 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using NBP2-61477 at 0.1 ug/ml](http://images.novusbio.com/fullsize/HA-Tag-Antibody-RM305-Western-Blot-NBP2-61477-img0003.jpg "Western Blot: HA Tag Antibody (RM305) [NBP2-61477] - 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using NBP2-61477 at 0.1 ug/ml")
![Immunohistochemistry-Paraffin: HA Tag Antibody (RM305) [NBP2-61477] - Staining of LNCaP cells overexpressing HA-tag Bag1 protein, using NBP2-61477 at 0.01 ug/ml](http://images.novusbio.com/fullsize/HA-Tag-Antibody-RM305-Immunohistochemistry-Paraffin-NBP2-61477-img0001.jpg)
![Western Blot: HA Tag Antibody (RMH02) [NBP2-61476] - Western blot of 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using NBP2-61476, followed by a HRP conjugated anti-Human IgG secondary antibody.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-RMH02-Western-Blot-NBP2-61476-img0003.jpg "Western Blot: HA Tag Antibody (RMH02) [NBP2-61476] - Western blot of 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using NBP2-61476, followed by a HRP conjugated anti-Human IgG secondary antibody.")
![ELISA: HA Tag Antibody (RMH02) [NBP2-61476] - Sandwich ELISA performed on whole lysate of LNCaP cells overexpressing HA-tag Bag1 protein. ELISA plate was coated with chimeric human anti-HA-Tag antibody Clone NBP2-61476 as the capture antibody. Anti-Bag1 rabbit serum was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the Secondary antibody.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-RMH02-ELISA-NBP2-61476-img0002.jpg)
![Western Blot: HA Tag Antibody (C.15200190) [NBP2-59172] - Whole cell extracts from HEK293 cells transfected with an HA-tagged expression vector using the antibody directed against HA at 1:1,000. Figure shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a HA-tagged protein (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-C-15200190-Western-Blot-NBP2-59172-img0001.jpg "Western Blot: HA Tag Antibody (C.15200190) [NBP2-59172] - Whole cell extracts from HEK293 cells transfected with an HA-tagged expression vector using the antibody directed against HA at 1:1,000. Figure shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a HA-tagged protein (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody.")