Alas1 Antibody (SA70-04)

Western Blot: Alas1 Antibody (SA70-04) [NBP2-67300] - Analysis of Alas1 on different cell lysates using anti-Alas1 antibody at 1/1,000 dilution. Positive control: Lane 1: JAR Lane 2: HUVEC Lane 3: Hela

Immunocytochemistry/Immunofluorescence: Alas1 Antibody (SA70-04) [NBP2-67300] - Staining Alas1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Alas1 Antibody (SA70-04) [NBP2-67300] - Staining Alas1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Alas1 Antibody (SA70-04) [NBP2-67300] - Staining Alas1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Alas1 Antibody (SA70-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Alas1 aa 510-640 / 640. (SwissProt: P13196 Human)
• Localization: Mitochondrion matrix
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ALAS1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Alas1 Antibody (SA70-04)

• 5-aminolevulinic acid synthase 1
• ALAS
• ALAS3
• ALASH
• ALAS-H
• aminolevulinate, delta-, synthase 1
• Delta-ALA synthase 1,5-aminolevulinate synthase, nonspecific, mitochondrial
• Delta-aminolevulinate synthase 1
• EC 2.3.1
• EC 2.3.1.37
• MIG4
• migration-inducing protein 4

Background

Delta-aminolevulinate synthase (ALAS; EC 2.3.1.37) catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2 (MIM 301300).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALAS1