Aldolase B Antibody

Western Blot: Aldolase B Antibody [NBP2-15344] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Aldolase B Antibody [NBP2-15344] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry: Aldolase B Antibody [NBP2-15344] - Hepatoma tissue; antibody dilution 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min.

Western Blot: Aldolase B Antibody [NBP2-15344] - A. 30 ug Neuro2A whole cell lysate/extract, B. 30 ug GL261 whole cell lysate/extract, C. 30 ug C8D30 whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.

Western Blot: Aldolase B Antibody [NBP2-15344] - 30 ug PC-12 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.

Western Blot: Aldolase B Antibody [NBP2-15344] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Aldolase B Antibody [NBP2-15344] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Re, Bv, Rb, Sh
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Aldolase B Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Aldolase B. The exact sequence is proprietary.
• Predicted Species: Bovine (91%), Rabbit (98%), Sheep (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALDOB
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Aldolase B Antibody

• ALDO2
• aldolase B, fructose-bisphosphate
• EC 4.1.2.13
• fructose-bisphosphatase
• fructose-bisphosphate aldolase B

Background

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ALDOB
• Uniprot:
  • P05062(Human)