Annexin A2 Antibody - BSA Free

Western Blot: Annexin A2 Antibody [NBP1-31310] - Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody [N1], N-term. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Annexin A2 Antibody [NBP1-31310] - Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody [N1], N-term. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Annexin A2 Antibody [NBP1-31310] - Analysis. Non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody [NBP1-31310] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Annexin A2 Antibody antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled Red.

Immunohistochemistry-Paraffin: Annexin A2 Antibody [NBP1-31310] - Human pancreas tissue, using ANXA2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: Annexin A2 Antibody [NBP1-31310] - A. 30 ug A549 whole cell lysate/extract, B. 30 ug HCT116 whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.

Immunohistochemistry-Paraffin: Annexin A2 Antibody [NBP1-31310] - Human pancreas tissue, using ANXA2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu, Rt, Po, Bv, Ca, Ch, Sh
• Applications: WB, ICC/IF, IHC, IP, KD, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Annexin A2 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Annexin A2. The exact sequence is proprietary.
• Localization: Secreted, extracellular space, extracellular matrix, basement membrane, Melanosome
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%), Chicken (93%), Canine (100%), Sheep (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANXA2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Application Notes: Knockdown validation (PMID: 31585940).
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31585940).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Annexin A2 Antibody - BSA Free

• Annexin A2
• Annexin II
• annexin-2
• ANX2
• ANX2L4
• ANX2L4LPC2
• ANXA2
• CAL1H
• Calpactin I heavy chain
• calpactin I heavy polypeptide
• Calpactin-1 heavy chain
• chromobindin 8
• chromobindin-8
• LIP2
• LIP2PAP-IV
• Lipocortin II
• Lipocortin-2
• LPC2D
• LPC2DP36
• p36
• PAP-IV
• Placental anticoagulant protein IV
• Protein I

Background

The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin II is a calcium regulated, membrane binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Annexin A2 Antibody (NBP1-31310)(8)

Publications using NBP1-31310

• Herrera-López EE, Guerrero-Escalera D, Aguirre-Maldonado I et al. Annexins A2 and A5 are potential early biomarkers of hepatocarcinogenesis Scientific reports 2023-04-28 [PMID: 37117324] (Immunocytochemistry/ Immunofluorescence)
• Weiss R, Bushi D, Mindel E et al. Autoantibodies to Annexin A2 and cerebral thrombosis: Insights from a mouse model Lupus 2021-02-07 [PMID: 33554716] (WB, Mouse)
• Lu H, Xie Y, Tran L et al. Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness J Clin Invest. 2020-05-19 [PMID: 32427586] (WB, IP, Human)
• Xiao Y, Zhao H, Tian L et al. S100A10 Is a Critical Mediator of GAS6/AXL-Induced Angiogenesis in Renal Cell Carcinoma Cancer Res 2019-10-06 [PMID: 31585940] (WB, Human)
• Liao L, Zheng B, Yi B et al. Annexin A2-modulated proliferation of pulmonary arterial smooth muscle cells depends on caveolae and caveolin-1 in hepatopulmonary syndrome Exp. Cell Res. 2017-07-17 [PMID: 28729092] (ICC/IF)
• Weiss R, Bitton A, Nahary L et al. Cross-reactivity between annexin A2 and Beta-2-glycoprotein I in animal models of antiphospholipid syndrome. Immunol. Res. 2016-07-23 [PMID: 27449504]
• Weiss R, Bitton A, Ben Shimon M et al. Annexin A2, autoimmunity, anxiety and depression J. Autoimmun. 2016-06-29 [PMID: 27372915]
• Rogers SW, Gahring LC. Upregulation of Nicotinic Acetylcholine Receptor alph4+beta2 through a Ligand-Independent PI3Kbeta Mechanism That Is Enhanced by TNFalpha and the Jak2/p38Mapk Pathways. PLoS One 2015-01-01 [PMID: 26619345] (ICC/IF)

FAQs for Annexin A2 Antibody (NBP1-31310). (Showing 1 - 1 of 1 FAQs).

1. I would like to know how the immunogen was produced and purified.  ..show answer..
   • The immunogen is a synthetic peptide, so it was not raised in any expression system. The peptide is conjugated to KLH prior to immunization.

Bioinformatics

• Gene Symbol: ANXA2
• Entrez:
  • 302(Human)
• Uniprot:
  • P07355(Human)