APH1A Antibody

Western Blot: APH1a Antibody [NBP2-27176] - analysis of APH1A in 1) human lung lysate using prebleed at 1:5000, 2) human lung lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu, Bv, Ca, Eq, Ma-Op, Pm, Mu, Rt, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

APH1A Antibody Summary

• Immunogen: A portion of amino acids 80-145 of human APH1A was used as the immunogen.
• Predicted Species: Mouse (100%), Rat (94%), Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APH1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 4-6ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 88% homologous in Chicken.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for APH1A Antibody

• 6530402N02Rik
• anterior pharynx defective 1 homolog A (C. elegans)
• APH-1
• APH1A
• APH-1A
• aph-1alpha
• CGI-78
• gamma-secretase subunit APH-1A
• Presenilin-stabilization factor
• PSF

Background

Gamma-secretase subunit APH1A (APH-1A) is a Notch signaling pathway protein and belongs to the APH-1 family. It is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It is likely a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. It is widely expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APH1A