Asporin Antibody - BSA Free

Western Blot: Asporin Antibody [NBP2-15492] - Comparative immunoblot analysis of normal wild type versus mdx-4cv liver extracts. Immunoblots with expanded views of labelling with antibodies to the mitochondrial outer membrane protein translocation pore subunit TOM22 (NBP1-80671) and asporin (NBP2-15492). Image collected and cropped by CiteAb from the following publication (//clinicalproteomicsjournal.biomedcentral.com/articles/10.1186/s12014-018-9212-2) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Asporin Antibody [NBP2-15492] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Western Blot: Asporin Antibody [NBP2-15492] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal diluted at 1:500.

Western Blot: Asporin Antibody [NBP2-15492] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal diluted at 1:500.

Western Blot: Asporin Antibody [NBP2-15492] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Comparative immunoblot analysis of normal wild type versus mdx-4cv liver extracts. Shown is a representative silver-stained SDS-PAGE gel & corresponding immunoblots with expanded views of labelling with antibodies to the fatty acid binding protein isoforms FABP1 & FABP5, as well as ferritin light chain, carbonic anhydrase isoform CA3, the voltage-dependent anion channel VDAC-1, the mitochondrial outer membrane protein translocation pore subunit TOM22, fibronectin & asporin. Lanes 1 & 2 represent total extracts from control wild type (wt) liver & mdx-4cv liver, respectively. Molecular mass standards (in kDa) are indicated at the left of the gel image. Graphical representations of the immuno-decoration levels of FABP1 & FABP5 are shown (Y-axis: % control): Student’s t test, unpaired; n = 4; *p < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30386187), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Asporin Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Asporin. The exact sequence is proprietary.
• Predicted Species: Porcine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ASPN
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Asporin Antibody - BSA Free

• asporin (LRR class 1)
• asporin
• FLJ20129
• OS3
• periodontal ligament associated protein 1
• Periodontal ligament-associated protein 1
• PLAP-1
• PLAP1SLRR1Casporin proteoglycan
• small leucine-rich protein 1C

Background

ASPN belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin (MIM 125255) (Lorenzo et al., 2001 [PubMed 11152692]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

&#9888; WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Asporin Antibody (NBP2-15492)(2)

Publications using NBP2-15492

• Murphy S, Zweyer M, Henry M et al. Proteomic profiling of liver tissue from the mdx-4cv mouse model of Duchenne muscular dystrophy Clin Proteom 2018-12-01 [PMID: 30386187] (WB, Mouse)
• Murphy S, Dowling P, Zweyer M et al. Proteomic analysis of dystrophin deficiency and associated changes in the aged mdx-4cv heart model of dystrophinopathy-related cardiomyopathy J Proteomics 2016-03-04 [PMID: 26961938] (WB, Mouse)

Bioinformatics

• Gene Symbol: ASPN
• Uniprot:
  • Q9BXN1(Human)