beta Tubulin Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Simple Western: beta Tubulin Antibody [NB600-936] - Lane view shows a specific band for beta Tubulin in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: beta Tubulin Antibody [NB600-936] - Analysis of extracts from HeLa, A549 and Jurkat cells using NB600-936 Beta-tubulin antibody at 1:1000

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - beta Tubulin Antibody [NB600-936] - Reduced levels of NADPH oxidases 1 and 4 at 18% versus 5% O2. (a) Representative Western blots showing Nox1 and Beta-tubulin in PC-3 cells or Nox4 and Beta-tubulin in C2C12 cells, at 5% and 18% O2. (b, c) Average Nox1 signal How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30363917/) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human skin using 1:2000 dilution of beta Tubulin antibody. Intense cytoplasmic staining of beta Tubulin (TUBB) protein was observed in various cells of the epidermal as well as the dermal cells [10X Magnification].

Western Blot: beta Tubulin Antibody [NB600-936] - Analysis in HeLa whole cell lysate at a 1:1,000 dilution.

Western Blot: beta Tubulin Antibody [NB600-936] - Expression of pluripotency related markers and a sphere culture of BEAS-2B under normoxia. HIF-2alpha expression was detected in the nucleus of BEAS-2B under normoxia by western blotting. Beta tubulin and TATA binding protein (TBP) were used as protein markers for the cytosol and nucleus fraction, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep29311), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Confocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Analysis of beta Tubulin in mouse hippocampal primary culture. Image courtesy of product review by Lin Yi-Wen.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. Strong cytoplasmic immuno-positivity of beta Tubulin (TUBB) was observed in SCC cells as well as the associated tumor stromal cells [10X Magnification].

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. This representative image shows a cytoplasm specific staining of beta Tubulin (TUBB) in SCC cells [60X Magnification].

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human brain using 1:2000 dilution of beta Tubulin antibody. The various brain cells depicted strong cytoplasmic immunoreactivity of beta Tubulin (TUBB) protein [10X Magnification].

Beta Tubulin was detected in immersion fixed A431 human skin carcinoma cell line using Rabbit anti-beta Tubulin Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB600-936AF647) (light blue) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Beta Tubulin was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rabbit anti-beta Tubulin Affinity Purified Polyclonal Antibody conjugated to DyLight 488 (Catalog # NB600-936G) (green) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Dual pathway inhibition regulates TK1 protein levels & results in greater p27 protein levels than single agents alone.Single agent PLX4032 resulted in activation of p-AKT Ser473 following 24 hours of exposure at two concentrations (100 nM, 1 µM). The addition of the dual PI3K/mTOR inhibitor BEZ235 blocks p-AKT Ser473 activation & resulted in a greater increase in p27 protein levels & diminished TK1 protein levels. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Expression of pluripotency related markers & a sphere culture of BEAS-2B under normoxia.(a) HIF-2 alpha expression was detected in the nucleus of BEAS-2B under normoxia by western blotting. Beta tubulin & TATA binding protein (TBP) were used as protein markers for the cytosol & nucleus fraction, respectively. (b) Western blotting for Oct-4. (c) For knockdown of HIF-2 alpha , BEAS-2B cells were transfected with shRNA targeting HIF-2 alpha & sh-Luc was used as control. (d) Western blotting of Nanog & a culture of BEAS-2B containing floating spheres. Representative result from two or three experiments was presented. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27373565), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Expression of AAV transgene before & after experimental autoimmune uveoretinitis (EAU) induction. (A) B10.RIII mice were evaluated by funduscopy one month after an intravitreal injection of AAV delivering sGFP-TatM013v5 or secreted GFP (sGFP). As a control we evaluated mice that received an intravitreal injection of saline. Diffuse fluorescence indicates the secretion of sGFP & sGFP-TatM013v5 in the retina. (B) Western blot from retinas harvested 14 days after IRBP immunization. Membrane was probed with anti-GFP & anti-Tubulin antibodies. Image shows expression of sGFP on both sGFP & sGFP-TatM013v5 retina lysates. (n = 2–3 retina samples from different mice per group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31795515), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Combined V600EBRAF & mTOR inhibition results in transcriptional control of TK1 protein levels in COLO 205 cells.(A) Western blot of COLO 205 cells treated with PP242 (250 nM) & increasing PLX4032. Similar to single agent PLX4032, p-MEK, but not p-ERK, was inhibited in a PLX4032-dependent manner. Consistent with mTORC1/mTORC2 inhibition, p-AKT Ser473, but not p-AKT Thr308, was inhibited. Unlike single agent PLX4032, which resulted in concentration-dependent activation of p-rpS6, combined treatment maintained p-rpS6 levels at essentially baseline levels except at the highest PLX4032 concentration. Similarly, DUSP6 levels were inversely related to p-ERK protein levels. With combined mTOR & V600EBRAF blockade, p27 & TK1 protein levels were inversely correlated & dramatically affected by PLX4032 exposure. (B) Similarly, TK1 mRNA was significantly reduced at PLX4032 concentrations as low as 10 nM. (C) Despite elevated p27 protein levels, p27 mRNA was unaffected by combined mTOR-V600EBRAF inhibition. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - [18F]-FLT PET reflects BEZ235-dependent inhibition of PI3K/mTOR activity in PLX4720 treated COLO 205 xenografts. Xenograft-bearing mice were imaged with [18F]-FLT PET on treatment day 4. (A) [18F]-FLT uptake was diminished in the combination treatment cohort relative to vehicle (p = 0.0087), but not single agent PLX4720- or BEZ235-treated cohorts. (B) Western blot of xenograft tissue harvested immediately following imaging illustrated elevated p-ERK & p-rpS6 levels in PLX4720-treated mice. Combining PLX4032 with BEZ235 resulted in reduced p-ERK & p-rpS6 protein levels. (C) TK1 levels, as measured by IHC, were reduced only in the combination treatment group in agreement with [18F]-FLT PET. (D) Consistent with in vitro studies, diminished TK1 levels, & consequently [18F]-FLT PET, correlated with elevated p27 that was elevated only in the combination treated group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - PLX4720 exposure does not affect [18F]-FLT PET in COLO 205 xenografts, despite evidence of target inhibition & diminished [18F]-FDG uptake.(A) Representative transverse [18F]-FLT & [18F]-FDG PET images acquired after three daily treatments with vehicle or 60 mg/kg PLX4720 (tumor indicated by arrowhead). (B) Quantification of PET data illustrated similar [18F]-FLT uptake in vehicle-treated & PLX4720-treated tumors. Unlike [18F]-FLT PET, PLX4720 exposure elicited a significant reduction in [18F]-FDG uptake (p = 0.0006). (C) Western blot analysis of vehicle- & PLX4720-treated tumor tissue confirmed that PLX4720 had no effect on TK1 protein levels in agreement with [18F]-FLT PET. Target inhibition as measured by p-MEK levels was observed. However, similar to in vitro studies, PLX4720-treated COLO 205 xenografts exhibited elevated p-ERK & p-rpS6 protein levels relative to vehicle controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Deletion of Phb1 affects lipid metabolism.Western blot (a) & quantification (b) of Acetyl-CoA carboxylase (ACC) & phosphorylated ACC (p-ACC) expression at P20. N = 6–7 animals per genotype. Unpaired two-tailed t-test [p-ACC (t = 0.4627, df = 11, p = 0.021), ACC (t = 1.355, df = 11, p = 0.26)]. Western blot (c) & quantification (d) of ACC & p-ACC expression at P40. N = 6–8 animals per genotype [p-ACC (t = 0.5447, df = 12, p = 0.42), ACC (t = 1.153, df = 12, p = 0.17)]. Unpaired two-tailed t-test. By RT-qPCR, we identified a significant downregulation of many enzymes involved with lipid biosynthesis at both P20 (e) & P40 (f): sterol regulatory element-binding protein 1 (Srebp1), 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), ATP citrate lyase (Acly), fatty acid synthase (FASN), acetyl-CoA carboxylase 2 (ACC2), N = 5 animals per genotype. Unpaired two-tailed t-test P20 [Srebp1 (t = 3.26, df = 8, p = 0.012), Hmgcr (t = 7.63, df = 8, p = 0.000061), Acly (t = 4.418, df = 8, 0.0022), FASN (t = 4.109, df = 8, p = 0.0034), ACC2 (t = 3.408, df = 8, p = 0.0092)]; P40 [Srebp1 (t = 7.551, df = 8, p = 0.000066), Hmgcr (t = 5.091, df = 8, p = 0.00094), Acly (t = 4.934, df = 8, p = 0.0011), FASN (t = 7.186, df = 8, p = 0.000094), ACC2 (t = 2.697, df = 8, p = 0.027)]. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. n.s. non-significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34078899), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Deletion of Phb1 affects lipid metabolism.Western blot (a) & quantification (b) of Acetyl-CoA carboxylase (ACC) & phosphorylated ACC (p-ACC) expression at P20. N = 6–7 animals per genotype. Unpaired two-tailed t-test [p-ACC (t = 0.4627, df = 11, p = 0.021), ACC (t = 1.355, df = 11, p = 0.26)]. Western blot (c) & quantification (d) of ACC & p-ACC expression at P40. N = 6–8 animals per genotype [p-ACC (t = 0.5447, df = 12, p = 0.42), ACC (t = 1.153, df = 12, p = 0.17)]. Unpaired two-tailed t-test. By RT-qPCR, we identified a significant downregulation of many enzymes involved with lipid biosynthesis at both P20 (e) & P40 (f): sterol regulatory element-binding protein 1 (Srebp1), 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), ATP citrate lyase (Acly), fatty acid synthase (FASN), acetyl-CoA carboxylase 2 (ACC2), N = 5 animals per genotype. Unpaired two-tailed t-test P20 [Srebp1 (t = 3.26, df = 8, p = 0.012), Hmgcr (t = 7.63, df = 8, p = 0.000061), Acly (t = 4.418, df = 8, 0.0022), FASN (t = 4.109, df = 8, p = 0.0034), ACC2 (t = 3.408, df = 8, p = 0.0092)]; P40 [Srebp1 (t = 7.551, df = 8, p = 0.000066), Hmgcr (t = 5.091, df = 8, p = 0.00094), Acly (t = 4.934, df = 8, p = 0.0011), FASN (t = 7.186, df = 8, p = 0.000094), ACC2 (t = 2.697, df = 8, p = 0.027)]. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. n.s. non-significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34078899), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - TK1 protein levels do not reflect p-ERK attenuation following inhibition of V600EBRAF inhibition in COLO 205 cells.COLO 205 cells were collected 48 hours of PLX 4032 exposure at 10 nM, 100 nM, 500 nM, 1 µM, or 5 µM. (A) Western blot analysis demonstrated target inhibition of p-MEK despite increased p-ERK levels. PI3K-mTOR signaling was elevated in a PLX 4032-dependent manner as exhibited by a steady rise in p-rpS6 levels. The ERK-phosphatase DUSP6 decreased in conjunction with mTOR signaling & was inversely proportional to p-ERK levels. A slight increase in p27 levels were observed concomitantly with only modest changes in TK1 levels, except at the highest dose of PLX4032. (B) Decreased TK1 mRNA levels were observed at all drug concentrations above 10 nM (p<0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Beta Tubulin was detected in immersion fixed NIH-3T3 Mouse fibroblast cell line using Rabbit anti-beta Tubulin Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB600-936AF647) (light blue) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ce, Ch, ChHa, I, In, Pm, Xp, Ze
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

beta Tubulin Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to the N-terminal region of human beta Tubulin (within residues 1-100). [Swiss-Prot: P07437]
• Localization: Major constituent of microtubules
• Marker: Microtubule Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TUBB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500 - 1:1000
  • Immunohistochemistry 1:1000 - 1:2000
  • Immunohistochemistry-Paraffin 1:1000 - 1:2000
  • Simple Western 1:1000. Use reported in scientific literature (PMID 35008713)
  • Western Blot 1:1000
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: separated by Size, antibody dilution of 1:500. Separated by Size-Wes, Sally Sue/Peggy Sue. SCW validated using murine pancreatic cancer cells.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate
  beta Tubulin Overexpression Lysate

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34519641). Invertebrate reactivity reported in scientific literature (PMID: 28114363). C. elegans reactivity reported in scientific literature (PMID: 27690361). Insect reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for beta Tubulin Antibody - BSA Free

• beta polypeptide
• beta Tubulin
• MGC117247
• OK/SW-cl.56
• TUBB
• TUBB5
• tubulin beta chain
• tubulin beta polypeptide
• tubulin beta-1 chain
• Tubulin beta-5 chain
• tubulin, beta

Background

The cytoskeleton is consists of three major type of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments, and tubulins comprises the microtubule building block. Tubulin is a globular dimeric protein of alpha/beta chains and it has five distinct forms labeled as -a, -b,-g, -d and -e tubulin. a/b tubulins generates heterodimers which multimerize to form microtubule filaments. Several b tubulin isoforms such as b1, b2, b3, b4, b5, b6 , b8 etc have been identified among which b1/b4 are present in cytosol and b2 is present in nuclei/nucleoplasm. d/e tubulins are associated with centrosomes, whereas the g tubulin forms the gammasome, which is required for nucleating microtubule filaments at centrosomes. Several cellular movements are mediated by microtubule action which includes the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, nerve-cell axon migration etc and these movements result from competitive microtubule polymerization/depolymerization or through the actions of microtubule motor proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta Tubulin Antibody (NB600-936)(95)

Publications using NB600-936

• Li X, Su Q, Pan Y Overcharged lipid metabolism in mechanisms of antitumor by Tremella fuciformis‑derived polysaccharide International journal of oncology 2023-01-01 [PMID: 36416344] (WB, Mouse)
  
  Details:
  Dilutions: 1:1000
• Kang S, Han Z, Daude N et al. Tau conformers in FTLD-MAPT undergo liquid-liquid phase separation and perturb the nuclear envelope bioRxiv 2020-07-06 (WB, Human)
• Hiral Shah, Marine Olivetta, Chandni Bhickta, Paolo Ronchi, Monika Trupinić, Eelco C Tromer, Iva M Tolić, Yannick Schwab, Omaya Dudin, Gautam Dey Life-cycle-coupled evolution of mitosis in close relatives of animals. Nature 2024-06-06 [PMID: 38778110]
• Kai Zhao, Madita Braun, Leonie Meyer, Katharina Otte, Hartmann Raifer, Frederik Helmprobst, Vincent Möschl, Axel Pagenstecher, Hans Urban, Michael W. Ronellenfitsch, Joachim P. Steinbach, Jelena Pesek, Bernhard Watzer, Wolfgang A. Nockher, R. Verena Taudte, Andreas Neubauer, Christopher Nimsky, Jörg W. Bartsch, Tillmann Rusch, Swapan K. Ray A Novel Approach for Glioblastoma Treatment by Combining Apoptosis Inducers (TMZ, MTX, and Cytarabine) with E.V.A. (Eltanexor, Venetoclax, and A1210477) Inhibiting XPO1, Bcl-2, and Mcl-1 Cells 2024-04-04 [PMID: 38607071]
• VerPlank JJ, Gawron JM, Silvestri NJ et Al. Knockout of PA200 improves proteasomal degradation and myelination in a proteotoxic neuropathy Life Sci Alliance 2024-02-06 [PMID: 38320810]
• Andrew R. Castle, Sang-Gyun Kang, Ghazaleh Eskandari-Sedighi, Serene Wohlgemuth, My-Anh Nguyen, Daniel J. Drucker, Erin E. Mulvihill, David Westaway Beta-endoproteolysis of the cellular prion protein by dipeptidyl peptidase-4 and fibroblast activation protein Proceedings of the National Academy of Sciences of the United States of America 2022-12-27 [PMID: 36574660]
• Otte K, Zhao K, Braun M et al. Eltanexor Effectively Reduces Viability of Glioblastoma and Glioblastoma Stem-Like Cells at Nano-Molar Concentrations and Sensitizes to Radiotherapy and Temozolomide Biomedicines 2022-08-31 [PMID: 36140245] (, )
• Kashio M, Masubuchi S, Tominaga M. Protein kinase C-mediated phosphorylation of transient receptor potential melastatin type 2 Thr738 counteracts the effect of cytosolic Ca(2+) and elevates the temperature threshold The Journal of Physiology 2022-10-01 [PMID: 36042566] (, )
• Del Rosario JS, Gabrielle M, Yudin Y, Rohacs T. TMEM120A/TACAN inhibits mechanically activated PIEZO2 channels Journal of General Physiology 2022-08-01 [PMID: 35819364] (, )
• D'Otolo V Functional complementarity of mammalian coronins determines T cell population homeostasis Thesis 2022-01-01 (WB, Human)
• BBLT L, Nabatanzi R, Mausser E et al. Diversity of Vaginal Microbiota Affects Epithelial Barrier Permeability Among African Pregnant Women Research Square Feb 22 2021 (WB, Human)
• Moore SM, Gawron J, Stevens M et al. Pharmacologically increasing cGMP improves proteostasis and reduces neuropathy in mouse models of CMT1 Cellular and Molecular Life Sciences: CMLS 2024-10-14 [PMID: 39400753]
• Wilson ER, Nunes GD, Shen S et Al. Loss of prohibitin 2 in Schwann cells dysregulates key transcription factors controlling developmental myelination Glia 2024-10-30 [PMID: 39215540]
• Affandi T, Haas A, Ohm A et al. PKC? regulates chromatin remodeling and DNA repair through SIRT6 bioRxiv 2023-05-25 [PMID: 37292592] (WB)
• Nunes GDF Elucidating the role of Schwann cell mitochondria in myelin maintenance Thesis 2021-01-01
• Robbins L, Balaram A, Dejneka S et al. Heterologous Production of the D-Cycloserine Intermediate O-acetyl-L-serine in Human Lung Cancer Cells Research Square 2022-10-24 [PMID: 37237156] (WB, Human)
• Li F, Liu Y, Li L et al. Brain-derived extracellular vesicles mediate traumatic brain injury associated multi-organ damage Biochemical and Biophysical Research Communications 2023-05-01 [PMID: 37163934] (ICC/IF, Mouse)
• Mouchbahani-Constance S, Lagard C, Schweizer J et al. Modulating the activity of human nociceptors with a SCN10A-promoter specific viral vector tool Neurobiology of Pain 2023-01-01 [PMID: 36816616]
• Ridley RB, Bowman BM, Lee J et al. Modulation of Retinal Inflammation Delays Degeneration in a Mouse Model of Geographic Atrophy bioRxiv : the preprint server for biology 2023-02-09 [PMID: 36798403] (WB, Mouse)
• Shackleford G, Marziali LN, Sasaki Y Et al. A new mouse model of Charcot-Marie-Tooth 2J neuropathy replicates human axonopathy and suggest alteration in axo-glia communication PLoS Genet 2022-11-09 [PMID: 36350884] (WB, Primate, Mouse)
  
  Details:
  Citation using the HRP version of this antibody.
• Bocek I, Hok L, Persoons L et al. Imidazo[4,5-b]pyridine derived tubulin polymerization inhibitors: Design, synthesis, biological activity in vitro and computational analysis Bioorganic chemistry 2022-07-16 [PMID: 35872398] (WB, Human)
• Castle AR, Wohlgemuth S, Arce L, Westaway D Investigating CRISPR/Cas9 gene drive for production of disease-preventing prion gene alleles PloS one 2022-06-07 [PMID: 35671288] (WB, Mouse)
• Moreau, M, Capallere, C, et al. Reconstruction of functional human epidermis equivalent containing 5%IPS-derived keratinocytes treated with mitochondrial stimulating plant extracts. Sci Rep [PMID: 35641783] (Simple Western, Human)
  
  Details:
  0.180555556
• Wang LH, Lin CY, Lin YM et al. Calpain-2 Mediates MBNL2 Degradation and a Developmental RNA Processing Program in Neurodegeneration The Journal of neuroscience : the official journal of the Society for Neuroscience 2022-06-22 [PMID: 35606145]
• Lu X, Ding F, Chen Y, Ke S et Al. Deficiency of C1QL1 Reduced Murine Ovarian Follicle Reserve Through Intraovarian and Endocrine Control Endocrinology 2022-05-13 [PMID: 35560215]
• SchAfer A, Evers L, Meier L et al. The Metalloprotease-Disintegrin ADAM8 Alters the Tumor Suppressor miR-181a-5p Expression Profile in Glioblastoma Thereby Contributing to Its Aggressiveness Frontiers in Oncology 2022-03-15 [PMID: 35371977] (WB)
• yang C, Dominique GM, Champion MM, Huber PW Remnants of the Balbiani body are required for formation of RNA transport granules in Xenopus oocytes iScience 2022-03-18 [PMID: 35243240] (Xenopus)
• Cook L, Sengelmann M, Winkler B et al. ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9 International journal of molecular sciences 2022-02-10 [PMID: 35216088] (WB, Human)
• Clabough E, Ingersoll J, Reekes T et al. Acute Ethanol Exposure during Synaptogenesis Rapidly Alters Medium Spiny Neuron Morphology and Synaptic Protein Expression in the Dorsal Striatum International journal of molecular sciences 2021-12-28 [PMID: 35008713] (Simple Western, Mouse)
• Zhao K, SchAfer A, Zhang Z et al. Inhibition of Carbonic Anhydrase 2 Overcomes Temozolomide Resistance in Glioblastoma Cells International Journal of Molecular Sciences 2021-12-23 [PMID: 35008590] (WB)
• Tristan C, Ormanoglu P, Slamecka J et al. Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells Stem Cell Reports 2021-12-03 [PMID: 34861164]
• Kang L, Li C, Yang Q et al. (64)Cu-labeled daratumumab F(ab')(2) fragment enables early visualization of CD38-positive lymphoma European Journal of Nuclear Medicine and Molecular Imaging 2022-04-01 [PMID: 34677626] (, )
• Della-Flora Nunes G, Wilson ER, Hurley E et al. Activation of mTORC1 and c-Jun by Prohibitin1 loss in Schwann cells may link mitochondrial dysfunction to demyelination eLife 2021-09-14 [PMID: 34519641] (WB, Rat)
• Kang SG, Han ZZ, Daude N et al. Pathologic tau conformer ensembles induce dynamic, liquid-liquid phase separation events at the nuclear envelope BMC Biology 2021-12-01 [PMID: 34503506] (WB)
• Izadifar A, Courchet J, Virga DM Et al. Axon morphogenesis and maintenance require an evolutionary conserved safeguard function of Wnk kinases antagonizing Sarm and Axed Neuron 2021-08-04 [PMID: 34384519] (WB)
• Hatzopoulos GN, KUkenshOner T, Banterle N et al. Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly Nature communications 2021-06-21 [PMID: 34155202] (WB, Human)
• Della-Flora Nunes G, Wilson ER, Marziali LN et al. Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells Nature communications 2021-06-02 [PMID: 34078899] (WB, Mouse)
• Chen Y, Tristan CA, Lu C et al. A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells Nat Methods 2021-05-04 [PMID: 33941937] (Simple Western)
• Wu YH, Yang Y, Chen CH et al. Aerobic glycolysis supports hepatitis B virus protein synthesis through interaction between viral surface antigen and pyruvate kinase isoform M2 PLoS pathogens 2021-03-01 [PMID: 33720996] (WB, Human)
• NI Weinstock, C Kreher, J Favret, D Nguyen, ER Bongarzone, L Wrabetz, M Laura Felt, D Shin Brainstem development requires galactosylceramidase and is critical for pathogenesis in a model of Krabbe disease Nat Commun, 2020-10-23;11(1):5356. 2020-10-23 [PMID: 33097716]
• Tristan CA, Ormanoglu P, Slamecka J et al. Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells bioRxiv 2020-08-03 [PMID: 32793899] (WB, Human)
• Wang H, Zhang Z, Xie X et al. Paracellular pathway-mediated Mycoplasma hyopneumoniae migration across porcine airway epithelial barrier under air-liquid interface conditions Infect. Immun. 2020-08-03 [PMID: 32747599]
• Reed CB, Frick LR, Weaver A et al. Deletion of Calcineurin in Schwann Cells Does Not Affect Developmental Myelination, But Reduces Autophagy and Delays Myelin Clearance after Peripheral Nerve Injury J. Neurosci. 2020-07-08 [PMID: 32641402]
• Weinstock NI, Shin D, Dhimal N et al. Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction Neuron 2020-04-28 [PMID: 32375064]
• Baxter, M, Chapagai, D Et al. Peptidomimetic Polo-Box-Targeted Inhibitors that Engage PLK1 in Tumor Cells and Are Selective against the PLK3 Tumor Suppressor. ChemMedChem 2020-06-17 [PMID: 32232973] (WB, Human)
• Contractor T, Harris C Loss of copy of MIR1-2 increases CDK4 expression in ileal neuroendocrine tumors. Oncogenesis 2020-03-20 [PMID: 32198354] (FLOW, Human)
• Sun R, Eriksson S, Wang L The expression and activity of thymidine kinase 1 and deoxycytidine kinase are modulated by hydrogen peroxide and nucleoside analogs Nucleosides Nucleotides Nucleic Acids 2020-03-19 [PMID: 32189555]
• Markovics A, Angyal A, et al. GPR119 Is a Potent Regulator of Human Sebocyte Biology. J Invest Dermatol 2020-10-01 [PMID: 32142797] (WB, Human)
• Longaretti A, Forastieri C, Gabaglio M et Al. Termination of acute stress response by the endocannabinoid system is regulated through lysine-specific demethylase 1-mediated transcriptional repression of 2-AG hydrolases ABHD6 and MAGL J. Neurochem. 2020-03-05 [PMID: 32141088]
• Beaulieu-Laroche L, Christin M, Donoghue A et al. TACAN Is an Ion Channel Involved in Sensing Mechanical Pain Cell 2020-02-17 [PMID: 32084332] (WB, Human)
• Kallupi M, Carrette LLG, Kononoff J et al. Nociceptin attenuates the escalation of oxycodone self-administration by normalizing CeA-GABA transmission in highly addicted rats Proc. Natl. Acad. Sci. U.S.A. 2020-01-28 [PMID: 31932450] (WB, Rat)
• Ridley RB, Young BM, Lee J et al. AAV Mediated Delivery of Myxoma Virus M013 Gene Protects the Retina against Autoimmune Uveitis J Clin Med 2019-11-29 [PMID: 31795515] (WB, Mouse)
• Li, Y, Xing, Q Et al. Activation of RXR by bexarotene inhibits inflammatory conditions in human rheumatoid arthritis fibroblast like synoviocytes. Int J Mol Med 2019-11-01 [PMID: 31545398] (WB, Human)
• Gyongyosi M, Lukovic D, Zlabinger K et al. Liposomal doxorubicin attenuates cardiotoxicity via induction of interferon-related DNA damage resistance Cardiovasc. Res. 2019-07-25 [PMID: 31346605] (WB, Porcine)
• Hannen R, Selmansberger M, Hauswald M et al. Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor Cancers (Basel) 2019-06-30 [PMID: 31262047] (WB, Human)
• Scharfenberg F, Helbig A, Sammel M et al. Degradome of soluble ADAM10 and ADAM17 metalloproteases Cell. Mol. Life Sci. 2019-06-17 [PMID: 31209506]
• Baraliakos X, Boehm H, Bahrami R et al. Pseudoginsenoside-F11 Protects against Transient Cerebral Ischemia Injury in Rats Involving Repressing Calcium Overload Neuroscience 2019-05-23 [PMID: 31129202] (ICC/IF, Rat)
• Lee BJ, Jun HO, Kim JH, Kim JH. Astrocytic cystine/glutamate antiporter is a key regulator of erythropoietin expression in the ischemic retina FASEB J. 2019-02-11 [PMID: 30742774] (WB, Human)
• Castle AR, Daude N, Gilch S, Westaway D. Application of high-throughput, capillary-based Western analysis to modulated cleavage of the cellular prion protein J. Biol. Chem. 2018-12-21 [PMID: 30578300] (WB, Mouse)
• Hannen R, Steffani M, Voellger B et al. Effects of anti-estrogens on cell invasion and survival in pituitary adenoma cells: A systematic study. J. Steroid Biochem. Mol. Biol. 2018-11-13 [PMID: 30439415] (WB, Rat)
• Stuart JA, Fonseca J, Moradi F et al. How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Oxid Med Cell Longev. 2018-09-30 [PMID: 30363917] (WB, Mouse, Human)
• Yang L, Chen L, Lu X et al. Peri-ovarian adipose tissue contributes to intraovarian control during folliculogenesis in mice Reproduction 2018-05-29 [PMID: 29844226] (WB, Mouse)
• Hernandez M. R, Davis M. B, et al. Condensin I protects meiotic cohesin from WAPL-1 mediated removal. PLoS Genet 2018-05-01 [PMID: 29768402] (WB, C. elegans)
• Mao Z, Yang L, Lu X, Tan A. C1QTNF3 in the murine ovary and its function in folliculogenesis. Reproduction. 2018-02-01 [PMID: 29438034] (WB, Mouse)
• Neggers JE, Kwanten B, Dierckx T et al. Target identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes Nature Communications 2018-02-05 [PMID: 29402884] (, )
• Tessa Lord, Melissa J. Oatley, Jon M. Oatley Testicular Architecture Is Critical for Mediation of Retinoic Acid Responsiveness by Undifferentiated Spermatogonial Subtypes in the Mouse Stem Cell Reports 2018-02-01 [PMID: 29398482]
• Schulte ML, Fu A, Zhao P et al. Pharmacological blockade of ASCT2-dependent glutamine transport leads to antitumor efficacy in preclinical models. Nat. Med. 2018-02-01 [PMID: 29334372] (WB, Human)
• Bengoetxea H, Rico-Barrio I, Ortuzar N et al. Environmental Enrichment Reverses Tyrosine Kinase Inhibitor-Mediated Impairment Through BDNF-TrkB Pathway Mol. Neurobiol. 2017-08-25 [PMID: 28842826] (Rat)
• Requejo C, Ruiz-Ortega JA, Bengoetxea H et al. Deleterious Effects of VEGFR2 and RET Inhibition in a Preclinical Model of Parkinson's Disease Mol. Neurobiol. 2017-08-24 [PMID: 28840516] (Rat)
• Requejo C, Ruiz-Ortega JA, Cepeda H et al. Nanodelivery of Cerebrolysin and Rearing in Enriched Environment Induce Neuroprotective Effects in a Preclinical Rat Model of Parkinson's Disease Mol. Neurobiol. 2017-08-24 [PMID: 28840482] (Rat)
• Gelmedin V, Morel M, Hahnel S et al. Evidence for Integrin - Venus Kinase Receptor 1 Alliance in the Ovary of Schistosoma mansoni Females Controlling Cell Survival. PLoS Pathog [PMID: 28114363] (ICC/IF, Invertebrate)
• Vercruysse T, De Bie J, et al. The Second-Generation Exportin-1 Inhibitor KPT-8602 Demonstrates Potent Activity against Acute Lymphoblastic Leukemia. Clin Cancer Res 2017-05-15 [PMID: 27780859] (Simple Western, Human)
• Lau AC, Zhu KP, Brouhard EA et al. An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in C. elegans males. Epigenetics Chromatin. 2016-10-25 [PMID: 27777629] (WB)
• Snyder MJ, Lau AC, Brouhard EA et al. Anchoring of Heterochromatin to the Nuclear Lamina Reinforces Dosage Compensation-Mediated Gene Repression. PLoS Genet. 2016-09-01 [PMID: 27690361]
• Neggers JE, Vanstreels E, Baloglu E et al. Heterozygous mutation of cysteine528 in XPO1 is sufficient for resistance to selective inhibitors of nuclear export Oncotarget 2016-10-18 [PMID: 27634897] (, )
• Tan LX, Toops KA, Lakkaraju A. Protective responses to sublytic complement in the retinal pigment epithelium Proc. Natl. Acad. Sci. U.S.A. 2016-08-02 [PMID: 27432952]
• Shah H, Olivetta M, Bhickta C et al. Life cycle-coupled evolution of mitosis in close relatives of animals bioRxiv 2023-05-10 (Immunocytochemistry/ Immunofluorescence)
• Lee MC, Huang HJ, Chang TH et al. Genome-wide analysis of HIF-2alpha chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions. Sci Rep 2016-07-04 [PMID: 27373565] (WB)
• Ali AA, Lee YR, Chen TC et al. Novel Anthra[1,2-c][1,2,5]Thiadiazole-6,11-Diones as Promising Anticancer Lead Compounds: Biological Evaluation, Characterization & Molecular Targets Determination. PLoS One 2016-04-21 [PMID: 27100886] (WB)
• Scott M R, Rubio M D et al. Protein Expression of Proteasome Subunits in Elderly Patients with Schizophrenia. Neuropsychopharmacology 2016-01-02 [PMID: 26202105] (WB, Rat)
• McKinley ET, Zhao P, Coffey RJ et al. 3'-Deoxy-3'-[18F]-Fluorothymidine PET Imaging Reflects PI3K-mTOR-Mediated Pro-Survival Response to Targeted Therapy in Colorectal Cancer. PLoS ONE. 2014-09-24 [PMID: 25247710] (WB, Mouse)
• Chen WH, Cheng SJ, Tzen JT et al. Probing Relevant Molecules in Modulating the Neurite Outgrowth of Hippocampal Neurons on Substrates of Different Stiffness. PLoS One 2013-12-30 [PMID: 24386192] (ICC/IF, Mouse)
• Miklosz A, Lukaszuk B, Baranowski M et al. Effects of Inhibition of Serine Palmitoyltransferase (SPT) and Sphingosine Kinase 1 (SphK1) on Palmitate Induced Insulin Resistance in L6 Myotubes. PLoS One 2013-12-23 [PMID: 24376889] (WB, Rat)
• Lu ZH, Kaliberov S, Sohn R et al. Transcriptional Targeting of Primary and Metastatic Tumor Neovasculature by an Adenoviral Type 5 Roundabout4 Vector in Mice. PLoS One 2013-12-23 [PMID: 24376772] (WB, Mouse)
• Orlicky DJ, Monks J, Stefanski AL, McManaman JL. Dynamics and molecular determinants of cytoplasmic lipid droplet clustering and dispersion. PLoS One 2013-06-25 [PMID: 23825572]
• Riva M, He Z, Kallberg E et al. Human S100A9 Protein Is Stabilized by Inflammatory Stimuli via the Formation of Proteolytically-Resistant Homodimers. PLoS One 2013-04-23 [PMID: 23626736] (WB, Human, Primate)
• McKinley ET, Smith RA, Zhao P et al. 3'-Deoxy-3'-18F-fluorothymidine PET predicts response to (V600E)BRAF-targeted therapy in preclinical models of colorectal cancer. Chem Biol Interact. 2013-03-25 [PMID: 23341544] (WB, Human)
• Jacek E, Fallon BA, Chandra A et al. Increased IFNalpha activity and differential antibody response in patients with a history of Lyme disease and persistent cognitive deficits J Neuroimmunol 2012-11-07 [PMID: 23141748] (WB, Mouse)
• Li Z, Zhao ZJ, Zhu XQ, Ren QS, Nie FF, Gao JM, Gao XJ, Yang TB, Zhou WL, Shen JL, Wang Y, Lu FL, Chen XG, Hide G, Ayala FJ, Lun ZR. Differences in iNOS and Arginase Expression and Activity in the Macrophages of Rats Are Responsible for the Resistance against T gondii Infection. PLoS One;7(4). 2012-01-01 [PMID: 22558235] (WB, Rat, Mouse)
• Kaucher AV, Oatley MJ, Oatley JM. NEUROG3 is a critical downstream effector for STAT3-regulated differentiation of mammalian stem and progenitor spermatogonia Biol Reprod 2012-05-31 [PMID: 22378757] (WB, Mouse)
• Kadiu I, Gendelman HE. Human immunodeficiency virus type 1 endocytic trafficking through macrophage bridging conduits facilitates spread of infection J Neuroimmune Pharmacol 2011-12-01 [PMID: 21789505] (ICC/IF, Human)
• Lin SY, Chen NT, Sun SP et al. The protease-mediated nucleus shuttles of subnanometer gold quantum dots for real-time monitoring of apoptotic cell death. J Am Chem Soc. 2010-06-23 [PMID: 20499915] (WB, Human)
• Reding SC, Stepnoski AL, Cloninger EW, Oatley JM. THY1 is a conserved marker of undifferentiated spermatogonia in the pre-pubertal bull testis. Reproduction;139(5):893-903. 2010-05-01 [PMID: 20154176] (WB, Bovine)
• Catalucci D, Zhang D-H, DeSantiago J et al. Akt regulates L-type Ca2+ channel activity by modulating Cav{alpha}1 protein stability. J Cell Biol;184(6):923-933. 2009-01-01 [PMID: 19307602]
• Rattner A, Toulabi L, Williams J et al. The genomic response of the retinal pigment epithelium to light damage and retinal detachment. J Neurosci. 2008-01-01 [PMID: 18815272]

Reviews for beta Tubulin Antibody (NB600-936) (7)

Average Rating: 4.4

(Based on 7 reviews)

We have 7 reviews tested in 4 species: Human, Mouse, Ae. aegypti, Other.

reviewed by: Adeline Williams WB Ae. aegypti 05/22/2021

Summary

• Application: Western Blot
• Sample Tested: A549 Nuclear and cytoplasmic, Sample Amount: 30ug,Midgut tissue,Ovary tissue
• Species: Ae. aegypti
• Lot: K

Comments

• Comments: To test both anti-histone H3 and anti-beta tubulin as markers for nuclear or cytoplasmic fractions, respectively, in Ae. aegypti mosquitoes, a western blot was performed using midguts or ovaries dissected from mosquitoes 48h post-bloodmeal. The cytoplasmic or nuclear fractions from each tissue type were obtained and protein concentration was quantified by A260/280. Roughly 30ug of tissue was loaded onto SDS/PAGE gels that were either transferred to PVDF membranes or stained by Coomassie. Membranes were blocked in TBST 5% milk for 2h. Both anti-histone H3 and anti-beta tubulin were added 1:1000 in blocking buffer and incubated O/N shaking at 4C. Proteins were detected by Alkaline Phosphatase. In Ae. aegypti ovaries, anti-histone H3 bound to protein roughly 15kDa in the nuclear fraction of ovaries but not the cytoplasmic fraction, while anti-beta-tubulin bound to protein roughly 55kDa in both cytoplasmic and nuclear fractions. In Ae. aegypti midguts, anti-histone H3 bound to 3 proteins between 10-15kDa strongly in the nuclear fraction and faintly in the cytoplasmic fraction-perhaps due to nuclear contamination in the cytoplasmic fraction. anti-beta-tubulin did not react well in midgut fractions. The beta-tubulin antibody was not successful for differentiating cytoplasmic from nuclear fractions in Ae. aegypti tissues. For your reference, protein ladder = pageruler plus prestained ThermoFisher

reviewed by: Verified Customer Simple Western Mouse 01/21/2016

Summary

• Application: Simple Western
• Sample Tested: whole brain homogenates from mice
• Species: Mouse

reviewed by: Liyong Zhang WB Mouse 07/01/2014

Summary

• Application: Western Blot
• Species: Mouse

reviewed by: Bryan Tinsley ICC Mouse 06/12/2014

Summary

• Application: Immunocytochemistry
• Species: Mouse

reviewed by: Bryan Tinsley WB Human 05/27/2014

Summary

• Application: Western Blot
• Species: Human

reviewed by: Verified Customer IHC Mouse 01/13/2012

Summary

• Application: Immunohistochemistry
• Sample Tested: Hippocampal Primary Culture
• Species: Mouse

reviewed by: Verified Customer WB Other 11/09/2011

Summary

• Application: Western Blot
• Sample Tested: Chicken cell line
• Species: Other

FAQs for beta Tubulin Antibody (NB600-936). (Showing 1 - 1 of 1 FAQs).

1. We are looking for an antibody which is a specific marker for Mouse Bronchial cilia in IHC(p) technique. Preferably not from a mouse host. Can you help?
   • According to the literature I have reviewed on bronchial cilia markers, the most commonly used marker is acetylated alpha tubulin (AcTub). Please see the relevant publications with images attached (Song et al. 2014, Schamberger et al. 2014). We have one antibody to AcTub that is validated for IHC-P and mouse (it is however also raised in mouse, hence mouse on mouse blocking step would be necessary): NB600-567. There are also some reports of using beta tubulin as cilial marker (Davis et al. 2015). For rabbit host antibodies, you may look at our best seller products like NB600-936 and NB100-56459

Bioinformatics

• Gene Symbol: TUBB
• Entrez:
  • 203068(Human)
  • 545486(Mouse)
  • 498736(Rat)
• Uniprot:
  • P07437(Human)