Brachyury Antibody - BSA Free

Western Blot: Brachyury Antibody [NBP2-24676] - Analysis of Brachyury in U87 cell lysate. Lane 1 shows pre-immune sera, lane 2 shows this antibody tested at 2 ug/mL.

Western Blot: Brachyury Antibody [NBP2-24676] - Analysis of Brachyury in U87 cell lysate. Lane 1 shows pre-immune sera, lane 2 shows this antibody tested at 2 ug/mL.

Immunohistochemistry-Paraffin: Brachyury Antibody [NBP2-24676] - Analysis of Brachyury in FFPE human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/mL.

• Reactivity: Hu, Mu, Pm, Rt, Ca, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Brachyury Antibody - BSA Free Summary

• Immunogen: A portion of amino acids 350-400 of human Brachyury was used as the immunogen.
• Marker: Mesoderm Marker
• Predicted Species: Rat (93%), Canine (93%), Monkey (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TBXT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:20 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 1 - 3 ug/mL

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28132888).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Brachyury Antibody - BSA Free

• brachyury protein
• Brachyury
• MGC104817
• Protein T
• SAVA
• T brachyury (mouse) homolog
• T brachyury homolog
• T Brachyury Transcription Factor
• T, brachyury homolog (mouse)
• T-Box Transcription Factor T
• TBXT
• TF
• TFT

Background

Brachyury / Bry is encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Brachyury Antibody (NBP2-24676)(7)

Publications using NBP2-24676

• ChadlY DM, Oleksijew AM, Coots kS et al. Full Factorial Microfluidic Designs and Devices for Parallelizing Human Pluripotent Stem Cell Differentiation. SLAS Technol. 2018-07-01 [PMID: 29995450] (ICC/IF, Human)
• Qu Y, Zhou B, Yang W et al. Transcriptome and proteome characterization of surface ectoderm cells differentiated from human iPSCs. Sci Rep. 2016-08-23 [PMID: 27550649] (ICC/IF, WB, Human)
• Yukawa H, Noguchi H, Hayashi S. Embryonic body formation using the tapered soft stencil for cluster culture device. Biomaterials. 2011-05-01 [PMID: 21354615] (ICC/IF)
  
  Details:
  Product cited: Brachyury (IMG-6533A). IF/ICC: Induced pluripotent stem (iPS) cells, Fig 1 & 2.
• Qu Y, Cui X, Sareen D et al. In vitro induction of mammary-like differentiation from human pluripotent stem cells Patent 2018-08-02 (WB, Human)
• Song MH, Choi SC, Noh JM et al. LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells Biomaterials 2021-09-21 [PMID: 34571434] (ICC/IF, Human)
• Indana D, Agarwal P, Bhutani N, Chaudhuri O Viscoelasticity and Adhesion Signaling in Biomaterials Control Human Pluripotent Stem Cell Morphogenesis in 3D Culture Advanced materials (Deerfield Beach, Fla.) 2021-09-09 [PMID: 34499389] (ICC/IF)
• Qu Y, Han B, Gao B et al. Differentiation of Human Induced Pluripotent Stem Cells to Mammarylike Organoids Stem Cell Reports. 2017-02-14 [PMID: 28132888] (WB, Mouse, Human)

Bioinformatics

• Gene Symbol: TBXT
• Uniprot:
  • O15178()