Butyrylcholinesterase/BCHE Antibody

Western Blot: Butyrylcholinesterase/BCHE Antibody [NBP2-89134] - Analysis of BCHE on human blood plasma using anti-BCHE antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Butyrylcholinesterase/BCHE Antibody [NBP2-89134] - Staining BCHE in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Butyrylcholinesterase/BCHE Antibody [NBP2-89134] - Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Butyrylcholinesterase/BCHE antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: Butyrylcholinesterase/BCHE Antibody [NBP2-89134] - Staining BCHE in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Butyrylcholinesterase/BCHE Antibody [NBP2-89134] - Analysis of paraffin-embedded human colon cancer tissue using anti-BCHE antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Butyrylcholinesterase/BCHE Antibody [NBP2-89134] - Analysis of paraffin-embedded human skin tissue using anti-BCHE antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Butyrylcholinesterase/BCHE Antibody [NBP2-89134] - Analysis of paraffin-embedded human liver tissue using anti-BCHE antibody. Counter stained with hematoxylin.

Product Discontinued

Butyrylcholinesterase/BCHE Antibody Summary

• Immunogen: Recombinant protein within Human Butyrylcholinesterase/BCHE aa 309-520 / 602. (SwissProt: P06276 Human)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCHE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Butyrylcholinesterase/BCHE Antibody

• Acylcholine acylhydrolase
• BCHE
• Butyrylcholine esterase
• Butyrylcholinesterase
• CHE1
• CHE1cholinesterase
• Choline esterase II
• cholinesterase 1
• E1
• EC 3.1.1.8
• Pseudocholinesterase

Background

Butyrylcholinesterase (BtChE, EC 3.1.1.8.) is synthetizised in the liver, and is predominantly found in serum, liver and pancreas. Butyrylcholinesterase is a tetrameric glycoprotein (molecular mass of 350 kDa), and consists of four subunits, each with molecular mass of 90 kDa (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCHE