Casein Kinase 2 alpha Antibody (2B6) - BSA Free

Western Blot: Casein Kinase 2 alpha Antibody (2B6) [NBP3-15160] - Positive WB detected in: NIH/3T3 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, U-87 whole cell lysate. All lanes: Casein Kinase 2 alpha antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 30 KDa. Observed band size: 45 KDa.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 2B6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Casein Kinase 2 alpha Antibody (2B6) - BSA Free Summary

• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from human Casein Kinase 2 alpha. UniProt ID (P68400).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CSNK2A1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Casein Kinase 2 alpha Antibody (2B6) - BSA Free

• Casein Kinase 2 alpha
• casein kinase 2, alpha 1 polypeptide
• casein kinase II alpha 1 subunit
• CK II alpha
• CK2 catalytic subunit alpha
• CK2A1
• CK2A1casein kinase II subunit alpha
• CKII
• CSNK2A1
• EC 2.7.11
• EC 2.7.11.1
• protein kinase CK2

Background

Casein kinase I (also designated CKI) and casein kinase II (also designated CKII) compose a family of serine/ threonine protein kinases which are present in all eukaryotes examined to date. CKI family members, which include CKIalpha, gamma, e and delta, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an alpha2beta2 or an alphaalpha'beta2 structure. The a catalytic subunit is stimulated by the beta regulatory subunit, which undergoes autophosphorylation. CKII activity is high in the cytosol and nucleus of proliferating and differentiating cells. CKII is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSNK2A1