Caspase-3 Antibody - (active/cleaved) - BSA Free

Immunohistochemistry: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Immunohistochemistry analysis of sectioned tumor tissues from the MDA-MB-231 study. Each section was subjected to the specified antibody followed by a biotinylated secondary antibody. Detection was done using a DAB Peroxidase HRP Substrate Kit (brown) followed by Hematoxylin counterstaining (purple). Images were obtained using inverted bright field microscopy. Sectioning results are representative of three individual tumors. Scale bar is 50 microns. Statistical analysis using One-Way ANOVA. *p<0.05 vs tumor volume of the control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-01230-4), licensed under a CC-BY license.

Immunohistochemistry: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Immunohistochemistry analysis of sectioned tumor tissues from the MDA-MB-231 study. Each section was subjected to the specified antibody followed by a biotinylated secondary antibody. Detection was done using a DAB Peroxidase HRP Substrate Kit (brown) followed by Hematoxylin counterstaining (purple). Images were obtained using inverted bright field microscopy. Sectioning results are representative of three individual tumors. Scale bar is 50 microns. Statistical analysis using One-Way ANOVA. *p<0.05 vs tumor volume of the control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-01230-4), licensed under a CC-BY license.

Western Blot: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - The protein expression levels of Bcl-2, Bax and cleaved caspase-3 were detected in the different groups using Western blotting and were normalized to the housekeeping gene GAPDH. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.14135), licensed under a CC-BY license.

Western Blot: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Cell apoptosis was detected by flow cytometric analysis, and the percentage of apoptotic cells transfected with miR-200c inhibitor or NC inhibitor and treated with AngII or PBS for 48 h was determined. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.14135), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Dog ischemic brain stained for Active/Cleaved Caspase-3 expression using Caspase-3 Antibody - (active/cleaved)( NB100-56113) at 1:2000. Staining is seen in the nuclei of dying neurons (black arrow) but not in the morphologically normal nuclei (open arrows). Caspase-3 expression in the nucleus is considered to be a marker of active/caspase-3 expression and apoptosis. Hematoxylin-eosin counterstain.

Immunohistochemistry-Paraffin: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Irradiated mouse spleen stained for Active/Cleaved Caspase-3 expression using Caspase-3 Antibody - (active/cleaved) (NB100-56113) at 1:2000. Staining is seen in the nuclei of a subset of the cell population. Caspase-3 expression in the nucleus is considered to be a marker of active/caspase-3 expression and apoptosis. Hematoxylin-eosin counterstain.

Antidepressants-mediated effect on cellular protein content. HT-22 cells were treated with antidepressants for 48 and 96 h and densitometry analysis of NF-kappa B (b), p16 (c), p21 (d), p27 (e), p53 (f), TRF1 (g), TRF2 (h), calnexin (i), NuMa (j), cleaved caspase 3 (k), Bcl-2 (l) was evaluated. Representative Western Blots are presented (a). Bars indicate SD, n = 3, ***/^^^p < 0.001, **/^^p < 0.01, */^p < 0.05, no indication—no statistical significance (one-way ANOVA and Dunnett’s a posteriori test)

Immunohistochemistry: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Pathology in DOX treated acute & recovery phase mice. Regions of myofiber loss & frank replacement fibrosis were noted, most commonly in atria (A, acute phase), & rarely in ventricles (D, recovery phase). These areas were accompanied by macrophage infiltration (B, E) & myofibroblast proliferation (C) consistent with fibroplasia. Rare myofibers were matrix metalloproteinase 2 (F, recovery phase animal) or caspase-3 positive (G, acute phase animal). Reticulin staining (A, D); Immunohistochemistry: Iba 1(B, E; macrophages), alpha SMA (C), MMP-2 (F) & cleaved caspase -3 (G) Bar = 100µm (A-C); 50µm (D-F); 20µm (G). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31263061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Antidepressants-mediated effect on cellular protein content. HT-22 cells were treated with antidepressants for 48 & 96 h & densitometry analysis of NF-kappa B (b), p16 (c), p21 (d), p27 (e), p53 (f), TRF1 (g), TRF2 (h), calnexin (i), NuMa (j), cleaved caspase 3 (k), Bcl-2 (l) was evaluated. Representative Western Blots are presented (a). Bars indicate SD, n = 3, ***/^^^p < 0.001, **/^^p < 0.01, */^p < 0.05, no indication—no statistical significance (one-way ANOVA & Dunnett’s a posteriori test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31278507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Morphological changes & expression of key apoptosis-related molecules in 4T1 cells after DE-EDCP treatment(A) Morphological changes of 4T1 cells exposed to various concentrations of DE-EDCP for 24h. (B) Immunofluorescence staining for Bcl-2 (green), Bax (green) & cleaved caspase-3 (green) together with DNA staining with DAPI (blue) in 4T1 cells incubated with DE-EDCP or cisplatin (31.25 μM) for 24h, as well as in untreated cells (magnification at x200). (C) mRNA expression of Bcl-2, Bax & caspase-3 quantified by RT-PCR in 4T1 cells after DE-EDCP 24h treatment. DE-EDCP treatment markedly increased the expression of Bax & caspase-3 mRNA & decreased the expression of Bcl-2 mRNA in 4T1 cells. beta -actin mRNA was used as an internal control. Data points are represented by the expression ratio & mean±SD fold of control in 4T1 cells. (* Bcl-2-: DE-EDCP vs. untreated p=0.03; DE-EDCP vs. cisplatin p=0.006; cislatin vs. untreated p=0.001; Bax-: DE-EDCP vs. untreated p=0.011; cislatin vs. untreated p=0.009; caspase-3-: DE-EDCP vs. untreated p=0.015; DE-EDCP vs. cisplatin p=0.021) Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.25610), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Ma
• Applications: WB, Flow, ICC/IF, IHC, IP, WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Caspase-3 Antibody - (active/cleaved) - BSA Free Summary

• Immunogen: This Caspase-3 Antibody - (active/cleaved) was developed against catalytically active human caspase-3 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular) reported in scientific literature (PMID 24804954)
  • Flow Cytometry reported in scientific literature (PMID 28287161)
  • Immunoblotting reported in scientific literature (PMID 27912032)
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 29963272)
  • Immunohistochemistry
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 29864441)
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
• Application Notes: Preferentially detects active caspase-3 (large subunit: ~14-21 kDa, and small subunit: ~10 kDa). However, it may also detect pro-caspase-3 (~32 kDa) in some cell or tissue systems. Nuclear immunostaining of caspase-3 is considered be an indication of active/cleaved caspase-3.
• Theoretical MW: 31.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Jurkat Staurosporine Treated / Untreated Cell Lysate

  Loading...

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34597692). Rat reactivity reported in scientific literature (PMID:32818590).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Caspase-3 Antibody - (active/cleaved) - BSA Free

• Apopain
• apoptosis-related cysteine protease
• CASP3
• CASP-3
• caspase 3, apoptosis-related cysteine peptidase
• Caspase3
• Caspase-3
• CPP32
• CPP-32
• CPP32B
• CPP32SREBP cleavage activity 1
• Cysteine protease CPP32
• EC 3.4.22
• EC 3.4.22.56
• LICE-1
• PARP cleavage protease
• procaspase3
• Protein Yama
• SCA-1
• YAMA

Background

CPP32 encodes a member of the cysteine-aspartic acid protease (caspase) family that plays a key role in apoptosis. Caspase 3 (also known as CPP32, Apopain, Yama, and PARP cleavage protease) is the most extensively studied apoptotic protein among caspase family members (1-2). Caspase 3 is synthesized as an inactive proenzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). Alternative splicing of CPP32 results in two transcript variants which encode the same protein. The processed form of Caspase 3 consists of large (theoretical molecular weight 17kD) and small (theoretical molecular weight 12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28/Ser29 and Asp175/Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Variations in levels of Caspase 3 have been reported in cells of short-lived nature and those with a longer life cycle. Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease (3).

References

1.Mu, N., Lei, Y., Wang, Y., Wang, Y., Duan, Q., Ma, G., . . . Su, L. (2019). Inhibition of SIRT1/2 upregulates HSPA5 acetylation and induces pro-survival autophagy via ATF4-DDIT4-mTORC1 axis in human lung cancer cells. Apoptosis, 24(9-10), 798-811. doi:10.1007/s10495-019-01559-3

2.Sun, C. M., Enkhjargal, B., Reis, C., Zhou, K. R., Xie, Z. Y., Wu, L. Y., . . . Zhang, J. H. (2019). Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats. CNS Neurosci Ther, 25(10), 1162-1172. doi:10.1111/cns.13199

3.Louneva, N., Cohen, J. W., Han, L. Y., Talbot, K., Wilson, R. S., Bennett, D. A., . . . Arnold, S. E. (2008). Caspase-3 is enriched in postsynaptic densities and increased in Alzheimer's disease. Am J Pathol, 173(5), 1488-1495. doi:10.2353/ajpath.2008.080434

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-3 Antibody (NB100-56113)(80)

Publications using NB100-56113

• Dragojevi? T, Živkovi? E, Dikli? M et Al. Hydroxyurea inhibits proliferation and stimulates apoptosis through inducible nitric oxide synthase in erythroid cells Biomed Pharmacother 2024-11-29 [PMID: 39615166]
• EKEBAS G, ATASEVER A, ALPAY A, YAMAN GRAM D Effect of rosemary extract (Rosmarinus officinalis) on chronic liver injury caused by carbon tetrachloride in rats and determination of hepatic apoptosis Sağlık Bilimleri Dergisi 2019-12-31
• Kaur, B;Miglioranza Scavuzzi, B;Yang, M;Yao, J;Jia, L;Abcouwer, SF;Zacks, DN; ER Stress and Mitochondrial Perturbations Regulate Cell Death in Retinal Detachment: Exploring the Role of HIF1? Investigative ophthalmology & visual science 2024-09-03 [PMID: 39325470]
• Mustafa Ahmed Abdel-Reheim, Merhan E. Ali, Ahmed Gaafar A. Gaafar, Ahmed Amine Ashour Quillaja saponin mitigates methotrexate-provoked renal injury; insight into Nrf-2/Keap-1 pathway modulation with suppression of oxidative stress and inflammation Journal of Pharmaceutical Health Care and Sciences 2024-04-09 [PMID: 38594773]
• Wang S, Liu A, Xu C, Hou J et Al. GLP-1(7-36) protected against oxidative damage and neuronal apoptosis in the hippocampal CA region after traumatic brain injury by regulating ERK5/CREB Mol Biol Rep 2024-02-20 [PMID: 38374452]
• Elkattawy HA, Mahmoud SM, Hassan AE et Al. Vagal Stimulation Ameliorates Non-Alcoholic Fatty Liver Disease in Rats Biomedicines 2023-12-08 [PMID: 38137476]
• Chandrakumar S, Santiago Tierno I, Agarwal M et al. Mechanical regulation of retinal vascular inflammation and degeneration in diabetes Diabetes 2023-11-21 [PMID: 37986627] (WB, Mouse)
• Wang S, Chang CW, Huang J et al. Gasdermin C sensitizes tumor cells to PARP inhibitor therapy in cancer models The Journal of clinical investigation 2023-10-26 [PMID: 37883181] (WB, Human)
• Abdeljalil SM, Wahdan SA, Elghazaly H, Tolba MF Insights into the therapeutic outcomes of trimetazidine/doxorubicin combination in Ehrlich solid-phase carcinoma mouse tumor model Life sciences 2023-06-21 [PMID: 37352914]
• Ruiz TFR, Grigio V, Ferrato LJ et al. Impairment of steroidogenesis and follicle development after bisphenol A exposure during pregnancy and lactation in the ovaries of Mongolian gerbils aged females Molecular and cellular endocrinology 2023-02-20 [PMID: 36813021]
• Elkhoely A Liraglutide ameliorates gentamicin-induced acute kidney injury in rats via PGC-1 alpha - mediated mitochondrial biogenesis: Involvement of PKA/CREB and Notch/Hes-1 signaling pathways International immunopharmacology 2022-12-14 [PMID: 36525794] (IF/IHC, Rat)
• Albadawy R, Hasanin AH, Agwa SHA et al. Rosavin Ameliorates Hepatic Inflammation and Fibrosis in the NASH Rat Model via Targeting Hepatic Cell Death International Journal of Molecular Sciences 2022-09-05 [PMID: 36077546] (Immunohistochemistry-Paraffin)
• Silconi ZB, Rosic V, Benazic S et al. The Pt(S-pr-thiosal)2 and BCL1 Leukemia Lymphoma: Antitumor Activity In Vitro and In Vivo International Journal of Molecular Sciences 2022-07-24 [PMID: 35897737] (Immunohistochemistry-Paraffin)
• Xu H, Ye W, Shi B LncRNA MALAT1 Regulates USP22 Expression Through EZH2-Mediated H3K27me3 Modification to Accentuate Sepsis-Induced Myocardial Dysfunction Cardiovascular toxicology 2022-06-20 [PMID: 35726125]
• Kelly YM, Ward C, Zhang R et al. Effects of Multi-Stage Procurement on the Viability and Function of Human Donor Parathyroid Glands The Journal of surgical research 2022-04-22 [PMID: 35468367] (IHC-P, Human)
• Chen L, Xu Y, Ye X Low temperature mitigating the paclitaxel-induced damages in mouse cell and hair follicle model Biochemical and biophysical research communications 2022-05-07 [PMID: 35279463] (IF/IHC, Mouse)
• Ma, N;Li, G;Fu, X; Protective role of activating transcription factor 3 against neuronal damage in rats with cerebral ischemia Brain and behavior [PMID: 35263513] (WB, Rat)
• Chen Q, Li Q, Liang Y Et al. Natural exosome-like nanovesicles from edible tea flowers suppress metastatic breast cancer via ROS generation and microbiota modulation Acta Pharmaceutica Sinica B 2021-08-01 [PMID: 35256954] (WB, Mouse, Human)
• Liu D, Tang X, Huang Z et al. Histone deacetylase HDAC2 regulates microRNA-125a expression in neuroblastoma Brain and behavior 2022-01-21 [PMID: 35060363] (Immunohistochemistry-Paraffin)
• Ruiz TFR, Leonel ECR, Colleta SJ et al. Gestational and lactational xenoestrogen exposure disrupts morphology and inflammatory aspects in mammary gland of gerbil mothers during involution Environmental toxicology and pharmacology 2022-01-01 [PMID: 34896274] (IHC-P, Gerbil)
• Saha A, Saleem S, Kumar PR, Biswas SC BH3-only proteins Puma and Beclin1 regulate autophagic death in neurons in response to Amyloid-beta Cell death discovery 2021-11-15 [PMID: 34782612] (ICC/IF, Rat)
• Zhao J, Dong L, Huo T Et al. O-GlcNAc Transferase (OGT) Protects Cerebral Neurons from Death During Ischemia/Reperfusion (I/R) Injury by Modulating Drp1 in Mice Neuromolecular medicine 2021-10-27 [PMID: 34705256]
• Ahmed ZSO, Galal MK, Drweesh EA et al. Protective effect of starch-stabilized selenium nanoparticles against melamine-induced hepato-renal toxicity in male albino rats International journal of biological macromolecules 2021-09-28 [PMID: 34597692] (IHC-P, Rat)
• Liu Z, Liu Q, Zhang B et al. Blood-Brain Barrier Permeable and NO-Releasing Multifunctional Nanoparticles for Alzheimer's Disease Treatment: Targeting NO/cGMP/CREB Signaling Pathways Journal of medicinal chemistry 2021-09-23 [PMID: 34517696]
• Senol N, Oguzoglu As, Erzurumlu Y Et Al. Modulation of Salubrinal-Mediated Endoplasmic Reticulum Stress in an Experimental Subarachnoid Hemorrhage Model World neurosurgery 2021-07-07 [PMID: 34245883] (IHC-P)
• Bashir D, Rashad M, Ahmed Y et al. The ameliorative effect of nanoselenium on histopathological and biochemical alterations induced by melamine toxicity on the brain of adult male albino rats NeuroToxicology 2021-07-01 [PMID: 34216684] (Rat)
• Todorovic Z, Milovanovic J, Arsenijevic D et al. Shikonin Derivatives from Onsoma visianii Decrease Expression of Phosphorylated STAT3 in Leukemia Cells and Exert Antitumor Activity Nutrients 2021-03-31 [PMID: 33807148] (ICC/IF, Mouse)
• Hashim AR, Bashir DW, Yasin NAE et al. Ameliorative effect of N-acetylcysteine against glyphosate-induced hepatotoxicity in adult male albino rats: histopathological, biochemical, and molecular studies Environmental science and pollution research international 2021-04-02 [PMID: 33797725] (IF/IHC, Rat)
• Mounier NM, Wahdan SA, Gad AM, Azab SS Role of inflammatory, oxidative, and ER stress signaling in the neuroprotective effect of atorvastatin against doxorubicin-induced cognitive impairment in rats Naunyn-Schmiedeberg's archives of pharmacology 2021-03-23 [PMID: 33755739]
• Codenotti S, Marampon F, Triggiani L et al. Caveolin-1 promotes radioresistance in rhabdomyosarcoma through increased oxidative stress protection and DNA repair Cancer letters 2021-02-18 [PMID: 33610729] (WB, Human)
• Tanita K, Sakura F, Nambu R, et al. Clinical and Immunological Heterogeneity in Japanese Patients with Gain-of-Function Variants in STAT3 Journal of clinical immunology 2021-01-26 [PMID: 33501615]
• Shaalan AK, Teshima THN, Tucker AS, Proctor GB. Inhibition of Aurora Kinase B activity disrupts development and differentiation of salivary glands Cell Death Discovery 2021-01-18 [PMID: 33462217] (Immunohistochemistry-Paraffin)
• Solek P, Shemedyuk N, Shemedyuk A et al. Risk of wild fungi treatment failure: Phallus impudicus-induced telomere damage triggers p21/p53 and p16-dependent cell cycle arrest and may contribute to male fertility reduction in vitro Ecotoxicology and environmental safety 2020-12-13 [PMID: 33321417] (WB)
• Enes Akkaya, Şevket Evran, Fatih Çalış, Serdar Çevik, Salim Katar, Ersin Karataş, Abdurrahim Koçyiğit, Mustafa Yasin Sağlam, Mustafa Aziz Hatiboğlu, Hakan Hanımoğlu, Mehmet Yaşar Kaynar Thymoquinone ameliorates delayed cerebral injury and cerebral vasospasm secondary to experimental subarachnoid haemorrhage. Neurologia i neurochirurgia polska 2021-01-07 [PMID: 33252137]
• Dhingani N, Guo C, Pan J et al. The E3 ubiquitin ligase UBR5 interacts with TTC7A and may be associated with very early onset inflammatory bowel disease Sci Rep 2020-10-29 [PMID: 33122718] (IF/IHC, Human)
• Tsaroucha AK, Korovesis GN, Valsami G et al. Silibinin-hydroxypropyl-beta-cyclodextrin (SLB-HP-beta-CD) complex prevents apoptosis in liver and kidney after hepatic ischemia-reperfusion injury Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2020-09-04 [PMID: 32891719] (IHC-P, Rat)
• Gao K, Niu J, Dang X Wnt-3a improves functional recovery through autophagy activation via inhibiting the mTOR signaling pathway after spinal cord injury Neurosci. Lett. 2020-08-17 [PMID: 32818590] (WB, Rat)
• Rahaman MS, Yamasaki S, Binte Hossain KF et al. Effects of curcumin, D-pinitol alone or in combination in cytotoxicity induced by arsenic in PC12 cells Food Chem. Toxicol. 2020-07-15 [PMID: 32679288] (WB, Rat)
• Rahaman MS, Banik S, Akter M et al. Curcumin alleviates arsenic-induced toxicity in PC12 cells via modulating autophagy/apoptosis Ecotoxicol. Environ. Saf. 2020-05-25 [PMID: 32464442] (WB, Rat)
• Davis JA, Bopp AC, Henwood MK et al. Pharmacological transection of brain-spinal cord communication blocks pain-induced hemorrhage and locomotor deficits after spinal cord injury in rats J. Neurotrauma 2020-05-05 [PMID: 32368946]
• Peng W, de Bruijn HS, Ten Hagen TLM et al. Targeted Photodynamic Therapy of Human Head and Neck Squamous Cell Carcinoma with Anti-epidermal Growth Factor Receptor Antibody Cetuximab and Photosensitizer IR700DX in the Mouse Skin-fold Window Chamber Model Photochem. Photobiol. 2020-03-29 [PMID: 32222965] (IF/IHC, Mouse)
• Wen S, Li S, Li L, Fan Q circACTR2: A Novel Mechanism Regulating High Glucose-Induced Fibrosis in Renal Tubular Cells via Pyroptosis Biol. Pharm. Bull. 2020-03-03 [PMID: 32115515] (WB, Human)
• Martins FF, Aguila MB, Mandarim-de-Lacerda CA Eicosapentaenoic and docosapentaenoic acids lessen the expression of PPAR gamma/Cidec affecting adipogenesis in cultured 3T3-L1 adipocytes Acta Histochem. 2020-01-16 [PMID: 31955908] (ICC/IF, Mouse)
• Shaalan A, Proctor G Salivary glands require Aurora Kinase B for regeneration after transient innate immune-mediated injury Sci Rep 2019-08-05 [PMID: 31383943] (WB, IHC-P, Mouse)
• Solek P, Koszla O, Mytych J et al. Neuronal life or death linked to depression treatment: the interplay between drugs and their stress-related outcomes relate to single or combined drug therapies Apoptosis 2019-07-05 [PMID: 31278507] (WB, Mouse)
• Zeiss C J, Gatti D M et al. Doxorubicin-Induced Cardiotoxicity in Collaborative Cross (CC) Mice Recapitulates Individual Cardiotoxicity in Humans. G3 (Bethesda) 2019-01-07 [PMID: 31263061] (IF/IHC, Mouse)
• Stojanovic B, Milovanovic J, Arsenijevic A et al. Galectin-3 Deficiency Facilitates TNF-a-Dependent Hepatocyte Death and Liver Inflammation in MCMV Infection Front Microbiol 2019-02-08 [PMID: 30800112] (IHC-P, Mouse)
• Codenotti S, Faggi F, Ronca R et al. Caveolin-1 enhances metastasis formation in a human model of embryonal rhabdomyosarcoma through Erk signaling cooperation Cancer Lett. 2019-02-13 [PMID: 30771426] (WB, Human)
• Corsetti G, Chen-Scarabelli C, Romano C et al. Autophagy and Oncosis/Necroptosis Are Enhanced in Cardiomyocytes from Heart Failure Patients Med Sci Monit Basic Res 2019-02-04 [PMID: 30713336] (IHC-P, Human)
• Hu S, Cheng M, Guo X et al. Down-regulation of miR-200c attenuates AngII-induced cardiac hypertrophy via targeting the MLCK-mediated pathway. J. Cell. Mol. Med. 2019-01-25 [PMID: 30680929] (WB, Rat)
• Martin KK, Parvin S, Garraway SM. Peripheral inflammation accelerates the onset of mechanical hypersensitivity after spinal cord injury and engages TNFa signaling mechanisms J. Neurotrauma 2018-12-06 [PMID: 30520675] (Immunohistochemistry-Paraffin)
• Dahiya NR, Chandrasekaran B, Kolluru V et al. A natural molecule, urolithin A, downregulates androgen receptor activation and suppresses growth of prostate cancer Mol. Carcinog. 2018-08-01 [PMID: 30069922] (WB, Human)
• Babic ZM, Zunic FZ, Pantic JM et al. IL-33 receptor (ST2) deficiency downregulates myeloid precursors, inflammatory NK and dendritic cells in early phase of sepsis J Biomed Sci. 2018-07-11 [PMID: 30001716] (IHC-P, Mouse)
• Jurisevic M, Arsenijevic A, Pantic J et al. The organic ester O,O’-diethyl-(S,S)-ethylenediamine-N,N’-di-2-(3-cyclohexyl)propanoate dihydrochloride attenuates murine breast cancer growth and metastasis Oncotarget. 2018-06-14 [PMID: 29963272] (ICC/IF, Mouse)
• Solek P, Majchrowicz L, Koziorowski M. Aloe arborescens juice prevents EMF-induced oxidative stress and thus protects from pathophysiology in the male reproductive system in vitro Environ. Res. 2018-06-07 [PMID: 29886390] (Mouse)
• Talreja D, Cashman SM, Dasari B et al. G-quartet oligonucleotide mediated delivery of functional X-linked inhibitor of apoptosis protein into retinal cells following intravitreal injection Exp. Eye Res. 2018-06-01 [PMID: 29864441] (IHC-Fr, Mouse)
• Guo Y, Wang F, Li H et al. Metformin Protects Against Spinal Cord Injury by Regulating Autophagy via the mTOR Signaling Pathway. Neurochem Res 2018-05-04 [PMID: 29728793] (Rat)
• Adamson Samantha E, Polanowska-Grabowska Renata, Marqueen Kathryn et al. Deficiency of Dab2 (Disabled Homolog 2) in Myeloid Cells Exacerbates Inflammation in Liver and Atherosclerotic Plaques in LDLR (Low-Density Lipoprotein Receptor)-Null Mice-Brief Report. Arteriosclerosis, Thrombosis, and Vascular Biology 2018-03-29 [PMID: 29599136] (IF/IHC, Mouse)
• Shaalan A, Carpenter G, Proctor G. Caspases are key regulators of inflammatory and innate immune responses mediated by TLR3 in vivo. Mol Immunol 2018-02-01 [PMID: 29331803] (Mouse)
• Bozec A, Zangari J, Butori-Pepino M, Ilie M et al. MiR-223-3p inhibits angiogenesis and promotes resistance to cetuximab in head and neck squamous cell carcinoma Oncotarget 2017-08-22 [PMID: 28915663] (IF/IHC, Human)
• Mytych J, Romerowicz-Misielak M, Koziorowski M. Long-term culture with lipopolysaccharide induces dose-dependent cytostatic and cytotoxic effects in THP-1 monocytes. Toxicol In Vitro. 2017-03-21 [PMID: 28341289]
• Solek P, Majchrowicz L, Bloniarz D et al. Pulsed or continuous electromagnetic field induce p53/p21-mediated apoptotic signaling pathway in mouse spermatogenic cells in vitro and thus may affect male fertility. Toxicology. 2017-03-16 [PMID: 28323003] (WB, Mouse)
• Phua T, Sng MK, Tan EH et al. Angiopoietin-like 4 Mediates Colonic Inflammation by Regulating Chemokine Transcript Stability via Tristetraprolin. Sci Rep. 2017-03-13 [PMID: 28287161] (FLOW, Mouse)
• Corsetti G, Romano C, Stacchiotti A et al. Endoplasmic Reticulum Stress and Apoptosis Triggered by Sub-Chronic Lead Exposure in Mice Spleen: a Histopathological Study. Biol Trace Elem Res. 2016-12-24 [PMID: 28012149] (Mouse)
• Salim E, Mahfouz M, Eltonouby E et al. Based polymer nanoparticles from bee pollen attenuate non-small lung cancer through enhancement of apoptosis and cell cycle arrest in vivo Research Square 2023-05-04 (IHC-P, Mouse)
• Chen L, Cheng H, Liao C et al. Luteolin improves nephropathy in hyperglycemic rats through anti-oxidant, anti-inflammatory, and anti-apoptotic mechanisms Journal of Functional Foods 2023-03-01 (WB, Rat)
• H Xiong, Z Ni, J He, S Jiang, X Li, J He, W Gong, L Zheng, S Chen, B Li, N Zhang, X Lyu, G Huang, B Chen, Y Zhang, F He LncRNA HULC triggers autophagy via stabilizing Sirt1 and attenuates the chemosensitivity of HCC cells. Oncogene 2017-09-15 [PMID: 28166203]
• Sidhar H, Giri RK. Induction of Bex genes by curcumin is associated with apoptosis and activation of p53 in N2a neuroblastoma cells. Sci Rep. 2017-02-01 [PMID: 28145533] (WB, Mouse)
• Rasheed Nora O Abdel, Ahmed Lamiaa A, Abdallah Dalaal M, El-Sayeh Bahia M Nephro-toxic effects of intraperitoneally injected EGCG in diabetic mice: involvement of oxidative stress, inflammation and apoptosis. Scientific Reports 2017-01-18 [PMID: 28098182] (IHC-P, Mouse)
• Haushalter C, Schuhbaur B, Doll� P, Rhinn M. Meningeal retinoic acid contributes to neocortical lamination and radial migration during mouse brain development Biology Open 2017-02-15 [PMID: 28011626] (Immunohistochemistry-Paraffin)
• Mytych J, Wos I, Solek P, Koziorowski M. Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes. Exp Cell Res 2017-01-15 [PMID: 28011195] (Human)
• Turtle JD, Strain MM, Aceves M et al. Pain Input Impairs Recovery after Spinal Cord Injury: Treatment with Lidocaine. J Neurotrauma 2017-03-15 [PMID: 27912032]
• Li T, Sun KJ, Wang HD et al. Tert-butylhydroquinone Ameliorates Early Brain Injury After Experimental Subarachnoid Hemorrhage in Mice by Enhancing Nrf2-Independent Autophagy. Neurochem. Res. 2015-09-01 [PMID: 26260377] (WB, Mouse)
• Garraway SM, Woller SA, Huie JR et al. Peripheral noxious stimulation reduces withdrawal threshold to mechanical stimuli after spinal cord injury: role of tumor necrosis factor alpha and apoptosis. Pain 2014-11-01 [PMID: 25180012]
• Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol. Int. 2014-05-07 [PMID: 24804954] (Flow Cytometry Control, Human)
  
  Details:
  Mouse splenocytes, human PBMC, Jurkat, EL4: Fig 5. GADPH was detected at ~36 kDa.
• Aulakh Gurpreet K, Balachandran Yadu, Liu Lixin, Singh Baljit. Angiostatin inhibits activation and migration of neutrophils. Cell Tissue Res. 2014-02-01 [PMID: 24297047]
• Tsai AC, Pai HC, Wang CY et al. In vitro and in vivo anti-tumour effects of MPT0B014, a novel derivative aroylquinoline, and in combination with erlotinib in human non-small-cell lung cancer cells. Br. J. Pharmacol. 2014-01-01 [PMID: 24116948] (WB, Human)
  
  Details:
  A549, Fig 4B-C. Both pro and cleaved caspase-3 was detected. The results showed B014 treatment triggered apoptosis and induced caspase-3 cleavage.
• Liu G, Keeler BE, Zhukareva V, Houle JD. Cycling exercise affects the expression of apoptosis-associated microRNAs after spinal cord injury in rats. Exp Neurol. 2010-11-01 [PMID: 20816819] (WB, Rat)
• Matsuda N, Teramae H, Futatsugi M et al. Up-regulation of histamine H4 receptors contributes to splenic apoptosis in septic mice: counteraction of the antiapoptotic action of nuclear factor-kappaB J Pharmacol Exp Ther 2010-03-01 [PMID: 20008488] (WB, Mouse)
• Sato K, Arai H, Miyazawa Y et al. Palatinose and oleic acid act together to prevent pancreatic islet disruption in nondiabetic obese Zucker rats. J Med Invest. 2008-08-01 [PMID: 18797130] (IHC-P)
  
  Details:
  Caspase-3 Active/Cleaved (IMG-5700). IF/ICC: Pancreatic islet cells from Zucker rats, the animals were fed a diet of carbohydrates and fats, Fig 5.

FAQs for Caspase-3 Antibody (NB100-56113). (Showing 1 - 1 of 1 FAQs).

1. I used your NB100-56113 and I was wondering if the positive signal could show up in the cytoplasm  ..show answer..
   • The caspase 3 protein is a cytoplasmic protein that can be transported from the cytoplasm to the nucleus during apoptosis, so it is possible that the antibody can be found in both locations.

Bioinformatics

• Gene Symbol: CASP3