Catalase Antibody (S05-5D9)

Western Blot: Catalase Antibody (S05-5D9) [NBP3-14915] - Western blot detection of Catalase in K562, Hela cell lysates using Catalase Rabbit mAb (1:1000 diluted). Predicted band size: 60kDa. Observed band size: 60kDa.

Immunocytochemistry/Immunofluorescence: Catalase Antibody (S05-5D9) [NBP3-14915] - Immunofluorescence of Catalase (green) in Hela cells using Catalase Rabbit mAb at dilution 1/200, and DAPI (blue)

Immunohistochemistry-Paraffin: Catalase Antibody (S05-5D9) [NBP3-14915] - Immunohistochemistry of Catalase in paraffin-embedded Human breast cancer tissue using Catalase Rabbit mAb at dilution 1/1

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: S05-5D9
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Catalase Antibody (S05-5D9) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Catalase (Uniprot#: P04040)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CAT
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/20-1/100
  • Immunohistochemistry 1/20-1/50
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000-1/10000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for Catalase Antibody (S05-5D9)

• Cas1
• CAT
• Catalase
• Cs-1
• EC 1.11.1.6
• MGC138422
• MGC138424

Background

Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1.5A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erthrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflamation) (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAT