CD39L4/ENTPD5 Antibody - BSA Free

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Analysis in human liver and skeletal muscle tissues using NBP1-85751 antibody. Corresponding ENTPD5 RNA-seq data are ...read more
Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.
Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Simple Western: CD39L4/ENTPD5 Antibody [NBP1-85751] - Simple Western lane view shows a specific band for CD39L4/ENTPD5 in 0.2 mg/ml of h. Liver lysate(s). This experiment was performed under reducing conditions using ...read more
Simple Western: CD39L4/ENTPD5 Antibody [NBP1-85751] - Electropherogram image of the corresponding Simple Western lane view. CD39L4/ENTPD5 antibody was used at 1:25 dilution on h. Liver lysate(s) respectively.
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Liver tissue

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Validated by:
       

Orthogonal Strategies

 

Order Details

CD39L4/ENTPD5 Antibody - BSA Free Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AGSTGTRIHVYTFVQKMPGQLPILEGEVFDSVKPGLSAFVDQPKQGAETVQGLLEVAKDSIPRSHWKKTPVVLKATAGLRLLPEHKAKALLFEVKEIFRKSPFLVPKGSVSIMDGSDEGILAWVTVNFLTGQLHGHRQETVGTL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ENTPD5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Simple Western 1:25
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in h. Liver lysate(s), separated by Size, antibody dilution of 1:25, apparent MW was 57 kDa.
Control Peptide
CD39L4/ENTPD5 Protein (NBP1-85751PEP)
Publications
Read Publication using NBP1-85751.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (87%). Reactivity reported in scientific literature (PMID: 24435307)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CD39L4/ENTPD5 Antibody - BSA Free

  • CD39 antigen-like 4
  • CD39L4
  • CD39L4NTPDase 5
  • CD39-like 4
  • EC 3.6.1
  • EC 3.6.1.42
  • EC 3.6.1.6
  • ectonucleoside triphosphate diphosphohydrolase 5
  • ENTPD5
  • ER-UDPase
  • GDPase ENTPD5
  • Guanosine-diphosphatase ENTPD5
  • MGC163357
  • MGC163359
  • NTPDase 5
  • NTPDase-5
  • Nucleoside diphosphatase
  • Pcph proto-oncogene protein
  • PCPH
  • proto-oncogene CPH
  • UDPase ENTPD5
  • Uridine-diphosphatase ENTPD5

Background

ENTPD5 is similar to E-type nucleotidases (NTPases)/ecto-ATPase/apyrases. NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD5 contains 4 apyrase-conserved regions which is characteristic of NTPases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-85751
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Publications for CD39L4/ENTPD5 Antibody (NBP1-85751)(1)

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ENTPD5