Coagulation Factor III/Tissue Factor Antibody (SN20-16)

Western Blot: Coagulation Factor III/Tissue Factor Antibody (SN20-16) [NBP2-67731] - Analysis of Tissue Factor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-95, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: mouse brain tissue lysateLane 2: U937 cell lysateLane 3: SH-SY5Y cell lysate

Immunocytochemistry/Immunofluorescence: Coagulation Factor III/Tissue Factor Antibody (SN20-16) [NBP2-67731] - Staining Tissue Factor in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody (SN20-16) [NBP2-67731] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Tissue Factor antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: Coagulation Factor III/Tissue Factor Antibody (SN20-16) [NBP2-67731] - Staining Tissue Factor in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Coagulation Factor III/Tissue Factor Antibody (SN20-16) [NBP2-67731] - Staining Tissue Factor in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody (SN20-16) [NBP2-67731] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Tissue Factor antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody (SN20-16) [NBP2-67731] - Analysis of paraffin-embedded human pancreas tissue using anti-Tissue Factor antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: SN20-16
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Coagulation Factor III/Tissue Factor Antibody (SN20-16) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human Coagulation Factor III/Tissue Factor aa 30-70. (SwissProt: P13726 Human; SwissProt: P20352 Mouse; SwissProt: P42533 Rat)
• Localization: Membrane,screted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: F3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Coagulation Factor III/Tissue Factor Antibody (SN20-16)

• CD142 antigen
• CD142
• coagulation factor III (thromboplastin, tissue factor)
• Coagulation Factor III
• F3
• FLJ17960
• TF
• TFA
• Thromboplastin
• Tissue Factor

Background

Tissue Factor encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Coagulation Factor III/Tissue Factor Antibody (NBP2-67731)(1)

Publication using NBP2-67731

• Cao W, Zhu MY, Lee SH Et al. Modulation of Cellular NAD+ Attenuates Cancer-Associated Hypercoagulability and Thrombosis via the Inhibition of Tissue Factor and Formation of Neutrophil Extracellular Traps International journal of molecular sciences 2021-11-08 [PMID: 34769515] (WB, Mouse)

FAQs for Coagulation Factor III/Tissue Factor Antibody (NBP2-67731). (Showing 1 - 1 of 1 FAQ).

1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
   • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Bioinformatics

• Gene Symbol: F3